Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RCG00_RS09020 Genome accession   NZ_CP133217
Coordinates   1598321..1600042 (-) Length   573 a.a.
NCBI ID   WP_308135540.1    Uniprot ID   A0AA51MU47
Organism   Thiothrix subterranea strain DNT52     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1593321..1605042
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCG00_RS08995 (RCG00_08995) opgC 1593723..1594904 (+) 1182 WP_308135536.1 OpgC domain-containing protein -
  RCG00_RS09000 (RCG00_09000) ubiE 1594850..1595602 (-) 753 WP_308135537.1 bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase UbiE -
  RCG00_RS09005 (RCG00_09005) - 1595687..1596082 (+) 396 WP_308135538.1 hypothetical protein -
  RCG00_RS09010 (RCG00_09010) pilD 1596087..1596962 (-) 876 WP_308135539.1 A24 family peptidase Machinery gene
  RCG00_RS09015 (RCG00_09015) pilC 1596977..1598227 (-) 1251 WP_202716153.1 type II secretion system F family protein Machinery gene
  RCG00_RS09020 (RCG00_09020) pilB 1598321..1600042 (-) 1722 WP_308135540.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RCG00_RS09025 (RCG00_09025) - 1600123..1601679 (-) 1557 WP_308135541.1 AAA family ATPase -
  RCG00_RS09030 (RCG00_09030) - 1601789..1602691 (+) 903 WP_202716156.1 Rpn family recombination-promoting nuclease/putative transposase -
  RCG00_RS09035 (RCG00_09035) tfpZ 1602837..1603604 (-) 768 WP_308135542.1 TfpX/TfpZ family type IV pilin accessory protein -
  RCG00_RS09040 (RCG00_09040) - 1603640..1604182 (-) 543 WP_308135543.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  RCG00_RS09045 (RCG00_09045) - 1604261..1604815 (-) 555 WP_374048253.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 63034.91 Da        Isoelectric Point: 6.9643

>NTDB_id=872529 RCG00_RS09020 WP_308135540.1 1598321..1600042(-) (pilB) [Thiothrix subterranea strain DNT52]
MIPLTSSIPLPGLARQLVVQDLLDKESATQAVQKARAEKVSLVEWMIRSRIVSTKDVAYAASLEYGLSMVDLDQISIPPP
IAALLSPEQMRKLNLIPLYQRGKNLMVGLADPTYLTNLDDVKFATGLIPEPVFVEFDKLQRYISSDDGMSGHAAQPHSGK
ASPTAAPVAAAKEISLDVRDLKTKDDEDPAVIEFVDKLLAHGIKSKASDIHIEIFEKNMRIRYRIDGILQVVATPPNDSA
QQLISRIKVLARMNISERRVPQDGRIRFTVSDKQKIDFRVNTLPTLFGEKVVMRILDSSNATLGVEKLGFEPKQQHDFMK
ALEKPDGMILVTGPTGSGKTVTLYTGLGILNTPEKNISTAEDPAEINMPGVNQVNVNAKVGLTFAEALRSFLRQDPDIIM
VGEIRDLETAEIAIKAAQTGHLVLSTLHTNSAPETLTRLMNMGVPTFNIASSIHLIIAQRLARRLCENCKKPSEVPRDVL
LQMGFHQEEIPALKLYEPCGCSECSRGYKGRVGIYQVMPISAEMGRMIMNGSNSLEIAEQSLKEGIADLRRSALNKVRQG
VTSLAELERVTSD

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=872529 RCG00_RS09020 WP_308135540.1 1598321..1600042(-) (pilB) [Thiothrix subterranea strain DNT52]
ATGATTCCGCTGACTTCTTCCATCCCCTTACCGGGGTTAGCCAGACAATTAGTTGTGCAAGACTTGCTCGACAAAGAGTC
GGCGACGCAAGCCGTGCAAAAAGCGCGTGCGGAAAAAGTCTCGCTGGTGGAATGGATGATCCGCTCACGCATCGTATCCA
CCAAAGACGTCGCCTACGCCGCCAGCCTCGAATACGGCCTCAGTATGGTCGACCTCGACCAAATCAGCATTCCGCCGCCG
ATTGCAGCCTTGCTCAGCCCAGAGCAAATGCGCAAACTCAACCTGATTCCCCTCTATCAGCGCGGCAAAAATCTCATGGT
GGGCTTGGCTGACCCCACGTATTTGACCAATCTGGATGATGTCAAATTTGCCACCGGCTTAATCCCCGAACCCGTTTTTG
TCGAATTCGACAAGCTGCAACGCTACATCAGCAGCGACGACGGCATGAGCGGGCACGCCGCCCAACCTCACAGCGGCAAA
GCCTCTCCTACTGCCGCGCCGGTAGCCGCTGCCAAAGAAATATCCCTAGACGTCCGCGATCTCAAAACCAAAGATGACGA
AGATCCCGCCGTCATTGAATTCGTCGACAAGCTGTTAGCTCATGGCATTAAATCCAAAGCGTCGGATATTCACATTGAAA
TCTTTGAAAAAAACATGCGCATCCGCTACCGGATCGACGGCATTTTGCAAGTCGTTGCCACCCCACCCAACGATTCCGCC
CAGCAATTAATTTCGCGCATCAAAGTACTGGCGCGGATGAACATTTCCGAACGCCGCGTCCCTCAAGACGGGCGTATTCG
CTTTACGGTCAGCGACAAGCAAAAAATCGACTTTCGTGTCAACACCCTCCCCACCCTGTTCGGTGAAAAAGTGGTTATGC
GGATACTTGACTCATCCAACGCCACGCTAGGGGTAGAAAAGCTCGGTTTTGAACCCAAGCAACAACACGATTTCATGAAA
GCGCTGGAAAAACCCGATGGCATGATTCTGGTAACAGGCCCCACCGGTAGCGGTAAAACCGTCACGCTATACACCGGTTT
AGGCATCCTCAACACCCCGGAAAAAAACATTTCCACTGCCGAAGACCCCGCCGAAATCAATATGCCCGGCGTGAACCAAG
TCAACGTCAACGCCAAAGTCGGGCTGACCTTTGCCGAAGCTCTGCGCTCTTTTTTGCGTCAAGACCCGGACATCATCATG
GTGGGGGAAATCCGCGACCTTGAAACCGCTGAAATTGCCATCAAAGCGGCACAAACCGGACACTTGGTGTTATCCACGTT
GCACACCAATAGCGCCCCGGAAACCTTGACCCGTTTGATGAACATGGGCGTGCCGACGTTCAATATCGCCTCCTCCATTC
ACTTAATTATTGCCCAACGCCTCGCCCGGCGCTTGTGTGAAAATTGCAAAAAACCCTCGGAGGTACCCCGCGACGTTTTG
CTGCAAATGGGCTTTCACCAAGAGGAAATTCCAGCCCTCAAGCTGTATGAGCCTTGCGGCTGTTCAGAATGTTCGCGCGG
CTACAAAGGGCGGGTAGGTATTTACCAAGTCATGCCGATTTCCGCCGAAATGGGGCGCATGATTATGAATGGCAGCAACT
CGCTGGAAATCGCTGAACAATCCCTCAAAGAAGGCATTGCGGATTTACGCCGTTCCGCGCTCAACAAAGTGCGCCAAGGC
GTCACCTCATTGGCTGAATTGGAGCGCGTCACTTCAGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(198-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

50.856

100

0.518
  pilB Acinetobacter baylyi ADP1

49.915

100

0.515
  pilB Legionella pneumophila strain ERS1305867

49.826

100

0.499
  pilF Neisseria gonorrhoeae MS11

48.057

98.778

0.475
  pilB Vibrio cholerae strain A1552

46.931

96.684

0.454
  pilB Vibrio parahaemolyticus RIMD 2210633

46.691

94.939

0.443
  pilB Vibrio campbellii strain DS40M4

46.239

95.113

0.44
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.56

95.288

0.377
  pilB/pilB1 Synechocystis sp. PCC 6803

34.903

100

0.375
  pilF Thermus thermophilus HB27

38.121

98.429

0.375