Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   RAM80_RS19355 Genome accession   NZ_CP133165
Coordinates   4133321..4135414 (+) Length   697 a.a.
NCBI ID   WP_416422059.1    Uniprot ID   -
Organism   Pseudomonas sp. App30     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4128321..4140414
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAM80_RS19335 (RAM80_19300) pilM 4130472..4131536 (+) 1065 WP_416422055.1 pilus assembly protein PilM Machinery gene
  RAM80_RS19340 (RAM80_19305) - 4131536..4132108 (+) 573 WP_416422056.1 PilN domain-containing protein -
  RAM80_RS19345 (RAM80_19310) pilO 4132105..4132764 (+) 660 WP_416422057.1 type 4a pilus biogenesis protein PilO -
  RAM80_RS19350 (RAM80_19315) pilP 4132761..4133285 (+) 525 WP_416422058.1 type 4a pilus biogenesis lipoprotein PilP -
  RAM80_RS19355 (RAM80_19320) pilQ 4133321..4135414 (+) 2094 WP_416422059.1 type IV pilus secretin PilQ Machinery gene
  RAM80_RS19360 (RAM80_19325) aroK 4135419..4135937 (+) 519 WP_416422060.1 shikimate kinase AroK -
  RAM80_RS19365 (RAM80_19330) aroB 4136069..4137166 (+) 1098 WP_416422061.1 3-dehydroquinate synthase -
  RAM80_RS19370 (RAM80_19335) - 4137181..4138800 (+) 1620 WP_416422062.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 697 a.a.        Molecular weight: 75046.24 Da        Isoelectric Point: 5.5728

>NTDB_id=872263 RAM80_RS19355 WP_416422059.1 4133321..4135414(+) (pilQ) [Pseudomonas sp. App30]
MNRMLSALGLSLWMALLSPALMAATLKTLDVAALPGDRVELKLTFDAPVPAPRGYTTDQPARIALDLPGVTNGLATKTRD
LGVGNARSVTVVEAKDRTRLIVNLTNLAPYGTRVEGNNLFVMIGQGASNLVGGSVARAPAPAAAAPVGALPRTAVARAAP
SRPVYGQPAGRAIRNVDFQRGEQGEGNVVLDLSDPSISPDIQEQGNKIVLTFPKTQLPEPLRVRLDVKDFATPVQFVNST
ASADTATVTIEPGGTFDYSVFQTDNKLTVSVRQVTAEDIARKNADRFVYTGQKLSLNFQDIDVRSVLQLIADFTNLNLVA
SDTVQGGITLRLQNVPWDQALDLVLKTKNLDKRKVGNVLLVAPAAEIAARERQELESQKQIEELAPLRRELLQVNYAKAA
DIAKLFQSVTNSKTKVDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVNYDKSLGVSWSG
ATNGSGSSKWTVAGTQTTSTNTFVDLGATSATSGIGLGYVSNNLTLDLQLSAMEATGNGEVVSQPKVVTSDKETARIIKG
TEVPYQESSSSGATSVAFKEASLSLEVTPQITPDNRIIMDVRVNKDEPDYANELNGVPPIKKNEVKAKVLVNDGETVVIG
GVFSNTQTKTTDKVPFLGDVPYLGRLFRSDTVSEAKTELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2094 bp        

>NTDB_id=872263 RAM80_RS19355 WP_416422059.1 4133321..4135414(+) (pilQ) [Pseudomonas sp. App30]
ATGAATAGGATGCTTTCAGCCCTTGGTCTCTCGCTATGGATGGCGCTGCTTTCGCCTGCCTTGATGGCGGCCACCCTGAA
AACACTGGATGTGGCGGCGTTGCCAGGGGACCGGGTTGAGCTCAAGCTGACCTTCGACGCCCCCGTGCCGGCGCCACGCG
GCTACACCACCGACCAGCCGGCGCGCATTGCCCTGGACCTGCCCGGGGTGACCAACGGCCTGGCTACCAAGACCCGTGAC
CTGGGCGTGGGCAACGCCCGCAGCGTGACCGTGGTGGAAGCCAAGGACCGCACCCGGCTGATCGTCAACCTCACCAACCT
GGCGCCCTACGGCACCCGCGTGGAAGGCAACAACCTGTTCGTGATGATTGGCCAGGGCGCCAGCAACCTGGTGGGTGGCT
CGGTGGCCCGTGCCCCGGCGCCTGCCGCCGCTGCCCCGGTTGGCGCGCTGCCACGCACTGCGGTGGCCCGTGCGGCGCCG
TCGCGCCCGGTGTACGGCCAGCCAGCCGGCCGCGCCATTCGCAACGTCGACTTCCAGCGCGGCGAGCAGGGCGAAGGCAA
CGTGGTGCTCGACCTCAGCGACCCAAGCATCAGCCCCGATATCCAGGAGCAGGGCAACAAGATCGTCCTGACCTTCCCCA
AGACCCAGCTGCCTGAGCCGCTGCGCGTGCGCCTGGACGTCAAGGACTTCGCCACCCCCGTGCAGTTCGTCAACTCCACG
GCCTCGGCGGACACCGCCACCGTGACCATCGAGCCGGGCGGCACCTTCGACTACTCGGTGTTCCAGACCGACAACAAGCT
GACCGTCAGCGTGCGCCAGGTCACCGCCGAGGACATCGCCCGCAAGAACGCCGATCGCTTCGTGTACACCGGCCAGAAGC
TGTCGCTGAACTTCCAGGACATCGACGTGCGTTCGGTGCTGCAACTGATCGCCGACTTCACCAACCTCAACCTGGTGGCC
AGTGACACCGTGCAGGGCGGCATCACCCTGCGCCTGCAGAACGTGCCGTGGGACCAGGCCCTGGACCTGGTGCTCAAGAC
CAAGAATCTCGACAAGCGCAAGGTCGGCAACGTGCTGCTGGTGGCGCCGGCGGCGGAAATCGCCGCCCGCGAGCGCCAGG
AGCTGGAGTCGCAGAAGCAGATCGAGGAACTGGCGCCGCTGCGTCGCGAGCTGCTGCAGGTGAACTACGCCAAGGCCGCC
GACATTGCCAAGCTGTTCCAGTCGGTGACCAACTCCAAGACCAAGGTGGACGAGCGCGGCTCGATCACCGTGGACGAGCG
CACCAACAACATCATCGCCTACCAGACCCAGGACCGCCTGGACGAACTACGCCGTATCGTCGCCCAGCTGGACATTCCGG
TGCGCCAGGTGATGATCGAGGCGCGCATCGTCGAGGCCAACGTCAACTACGACAAGAGCCTGGGCGTGAGCTGGAGCGGT
GCGACCAACGGCTCGGGCAGCAGCAAGTGGACCGTGGCCGGCACCCAGACCACCAGCACCAACACCTTCGTCGACCTGGG
CGCCACCAGCGCCACCTCGGGCATCGGCCTGGGCTATGTGTCCAACAACCTGACCCTGGACCTGCAGCTCAGTGCCATGG
AAGCCACCGGCAACGGCGAAGTGGTGTCGCAGCCCAAGGTGGTGACCTCGGACAAGGAAACCGCGCGCATCATCAAGGGC
ACCGAAGTGCCGTACCAGGAGTCCTCGTCCAGCGGTGCCACCTCGGTGGCGTTCAAGGAGGCCTCGCTGTCGCTGGAGGT
GACCCCGCAGATCACCCCGGACAACCGCATCATCATGGACGTGAGGGTCAACAAGGATGAGCCCGATTACGCCAACGAGC
TCAACGGCGTGCCGCCGATCAAGAAGAACGAAGTGAAGGCCAAGGTCCTCGTCAATGATGGCGAAACAGTGGTAATTGGC
GGTGTTTTCTCCAATACCCAAACTAAAACGACAGACAAGGTGCCATTTCTCGGTGATGTGCCGTATCTTGGCCGCCTGTT
TCGATCGGATACCGTGTCTGAGGCAAAAACCGAGCTGTTGGTCTTCCTCACTCCGCGTATCATGAATAACCAGGCGATTG
CTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(317-363)

AMIN domain (AMIN)

(39-121)

Bacterial type II/III secretion system short domain (Secretin_N)

(391-456)

Bacterial type II and III secretion system protein (Secretin)

(532-688)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.897

100

0.737
  comQ Acinetobacter baylyi ADP1

41.926

100

0.425
  pilQ Acinetobacter baumannii strain A118

38.718

100

0.416
  pilQ Acinetobacter baumannii D1279779

38.718

100

0.416
  pilQ Legionella pneumophila strain Lp02

38.171

98.852

0.377
  pilQ Legionella pneumophila strain ERS1305867

38.171

98.852

0.377