Detailed information NTG0872262

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: RAM80_RS19335
• Genome accession: NZ_CP133165
• Coordinates: 4130472..4131536 (+)
• Length: 354 a.a.
• NCBI ID: WP_416422055.1
• Uniprot ID: -
• Organism: Pseudomonas sp. App30
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4125472..4136536

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RAM80_RS19320 (RAM80_19285)	rpmE	4125938..4126156 (+)	219	WP_158154476.1	50S ribosomal protein L31	-
RAM80_RS19325 (RAM80_19290)	-	4126414..4127682 (+)	1269	WP_416422053.1	malic enzyme-like NAD(P)-binding protein	-
RAM80_RS19330 (RAM80_19295)	-	4127857..4130289 (-)	2433	WP_416422054.1	penicillin-binding protein 1A	-
RAM80_RS19335 (RAM80_19300)	pilM	4130472..4131536 (+)	1065	WP_416422055.1	pilus assembly protein PilM	Machinery gene
RAM80_RS19340 (RAM80_19305)	-	4131536..4132108 (+)	573	WP_416422056.1	PilN domain-containing protein	-
RAM80_RS19345 (RAM80_19310)	pilO	4132105..4132764 (+)	660	WP_416422057.1	type 4a pilus biogenesis protein PilO	-
RAM80_RS19350 (RAM80_19315)	pilP	4132761..4133285 (+)	525	WP_416422058.1	type 4a pilus biogenesis lipoprotein PilP	-
RAM80_RS19355 (RAM80_19320)	pilQ	4133321..4135414 (+)	2094	WP_416422059.1	type IV pilus secretin PilQ	Machinery gene
RAM80_RS19360 (RAM80_19325)	aroK	4135419..4135937 (+)	519	WP_416422060.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37874.38 Da
• Isoelectric Point: 4.4882

>NTDB_id=872262 RAM80_RS19335 WP_416422055.1 4130472..4131536(+) (pilM) [Pseudomonas sp. App30]
MLGLFGKKANSLLGIDISSTSVKLLELSRSGNRFRVEAYAVEPLPANAVVEKNIAELEGVGQALSRVLVKARTSAKTVAV
AVAGSAVITKTIEMDAALSEDEMENQLKVEADQYIPYPLEEVAIDFEVQGYSARNPERVDVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERAFGLLEPTLMEGEGPLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLSM
EEAGLAKKQGGLPDDYVAEVLQPFKDAVVQQVSRSLQFFFAAGQHNDVDYILLAGGTASISGLDQLIQQRLGTPAMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=872262 RAM80_RS19335 WP_416422055.1 4130472..4131536(+) (pilM) [Pseudomonas sp. App30]
GTGCTTGGACTCTTCGGCAAGAAAGCAAATTCGCTTTTGGGGATCGACATCAGCTCGACATCGGTGAAGCTTCTGGAGCT
CAGTCGCTCCGGCAACCGGTTCAGAGTCGAAGCCTACGCGGTTGAGCCCCTCCCGGCCAACGCGGTGGTGGAAAAGAACA
TCGCGGAGCTCGAGGGCGTGGGCCAGGCCTTGTCACGCGTGCTGGTCAAGGCCCGCACCAGCGCCAAGACCGTGGCCGTG
GCGGTCGCAGGTTCCGCTGTCATTACCAAGACCATCGAGATGGACGCTGCGCTTTCCGAAGACGAAATGGAAAACCAGCT
CAAGGTCGAGGCCGATCAATACATTCCCTACCCGCTTGAAGAGGTCGCCATCGACTTCGAGGTGCAGGGCTATTCGGCGC
GCAACCCCGAGCGGGTGGACGTGCTGCTGGCGGCCTGCCGCAAGGAAAACGTCGAGGTGCGCGAGGCGGCCCTGGCCCTG
GCTGGCCTGACCGCGCGCGTGGTCGACGTCGAGGCCTACGCCCTGGAGCGCGCTTTCGGCCTGCTGGAACCGACGCTGAT
GGAAGGCGAGGGCCCGCTGACCGTGGCCGTGGTCGACATCGGCGCCACCATGACCACCCTCAGCGTGCTGCACAACGGCC
GCATCATCTACACCCGCGAACAGCTGTTCGGCGGCCGCCAGCTCACCGAGGAGATCCAGCGCCGCTACGGGCTGTCCATG
GAAGAGGCGGGCCTGGCCAAGAAGCAGGGCGGCCTGCCTGACGACTACGTGGCCGAGGTGCTGCAGCCGTTCAAGGACGC
CGTGGTGCAGCAGGTCTCGCGCTCGCTGCAGTTCTTCTTCGCTGCCGGCCAGCACAACGACGTCGACTACATCCTGCTGG
CCGGTGGCACCGCCTCCATTTCCGGCCTCGACCAGCTGATCCAGCAGCGCCTGGGCACCCCGGCCATGGTCGCCAACCCC
TTTGCCGACATGGCCCTGAGCAGCAAGGTCAACGCCGGCGCCCTGGCCAGTGACGCGCCAGCGCTGATGATTGCCTGCGG
CCTGGCGTTGAGGAGTTTTGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	57.062	100	0.571
comM	Acinetobacter nosocomialis M2	57.062	100	0.571
comM	Acinetobacter baylyi ADP1	54.52	100	0.545
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466