Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RA164_RS02930 Genome accession   NZ_CP132911
Coordinates   710423..712144 (-) Length   573 a.a.
NCBI ID   WP_329742483.1    Uniprot ID   -
Organism   Dyella aluminiiresistens strain A6     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 705423..717144
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RA164_RS02910 - 705471..707228 (-) 1758 WP_329742479.1 glutamine--tRNA ligase/YqeY domain fusion protein -
  RA164_RS02915 coaE 707370..707990 (-) 621 WP_329742480.1 dephospho-CoA kinase -
  RA164_RS02920 pilD 707987..708853 (-) 867 WP_329742481.1 A24 family peptidase Machinery gene
  RA164_RS02925 pilC 708975..710243 (-) 1269 WP_329742482.1 type II secretion system F family protein Machinery gene
  RA164_RS02930 pilB 710423..712144 (-) 1722 WP_329742483.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RA164_RS02935 - 712417..713271 (+) 855 WP_329742484.1 glycosyltransferase family 2 protein -
  RA164_RS02940 - 713264..714457 (+) 1194 WP_329742485.1 glycosyltransferase family 4 protein -
  RA164_RS02945 - 714617..715639 (+) 1023 WP_329742486.1 glycosyltransferase family 4 protein -
  RA164_RS02950 - 715800..716897 (+) 1098 WP_329742487.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 62649.17 Da        Isoelectric Point: 5.4355

>NTDB_id=870586 RA164_RS02930 WP_329742483.1 710423..712144(-) (pilB) [Dyella aluminiiresistens strain A6]
MSSQVQPTLLGLSSIARRLVTEGLMPEDDVRQAMADASQRKVTLTTWLLDHNLVDGSRLTQLASVEFGMPMMDVSSIQTA
GMPINLISEALLSKHRVLPLLKRGKRLFVGIADPMQLHALDEIKFHSNCMVEPILVERSQLNRAIDSAISALSNTMPDLG
GSELDELALESGDDEVESTGVDANAGDDAPVVKFVNKILVDAIRRGASDIHFEPFETQYRVRLRMDGMLRAVASPPMKLA
NRISSRLKVMAGLDIAERRVPQDGRIKLNLGKNRSMDFRVSTLPTLFGEKIVLRILDSASAKLGIDKLGYEEDQKQLYLD
AIAKPYGMVLVTGPTGSGKTVSLYTALNILNTDERNISTVEDPVEIRVEGINQVQQNTKRGMTFAAALKSFLRQDPDVIM
VGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLIIAQRLARRLHSCKRPIDLPPQALL
DAGFTQEEIDAGLTVYEAVGCDECNEGYKGRVGIYQVMPMAEEIQKIVLQGGNALQIAEVASQAGINDLRASALKKVKDG
VTSLTEIDRVTKD

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=870586 RA164_RS02930 WP_329742483.1 710423..712144(-) (pilB) [Dyella aluminiiresistens strain A6]
ATGTCGTCGCAAGTACAACCCACGCTGTTAGGCCTGTCCAGCATCGCTCGGCGCCTGGTCACCGAGGGACTGATGCCCGA
GGACGACGTGCGCCAGGCGATGGCGGATGCGTCCCAGCGCAAAGTCACCCTGACCACCTGGCTGCTCGATCACAACCTGG
TCGACGGTTCGCGTCTGACTCAGCTGGCTTCGGTCGAATTCGGCATGCCGATGATGGATGTCTCGTCCATCCAGACTGCC
GGCATGCCGATCAACCTGATCAGCGAAGCGCTGCTGAGCAAGCACCGTGTGCTGCCTTTGCTCAAGCGCGGCAAGCGACT
GTTCGTAGGCATCGCCGACCCGATGCAGCTGCATGCGCTGGACGAGATCAAGTTCCACTCCAACTGCATGGTGGAACCCA
TCCTGGTCGAGCGCAGCCAGCTCAATCGTGCGATCGACAGTGCCATCAGCGCGCTCAGCAACACAATGCCCGACCTGGGC
GGCAGCGAACTGGACGAGCTGGCGCTCGAATCGGGCGACGACGAGGTCGAATCCACCGGTGTCGACGCCAACGCGGGCGA
TGACGCACCGGTCGTCAAATTCGTCAACAAGATCCTGGTGGACGCCATCCGGCGCGGCGCCTCCGACATCCACTTCGAAC
CGTTCGAGACACAGTACCGGGTGCGCCTGCGCATGGACGGCATGCTGCGCGCGGTCGCCAGTCCGCCGATGAAGCTGGCC
AACCGCATTTCGTCGCGCCTGAAGGTGATGGCCGGCCTGGATATCGCCGAACGCCGCGTTCCGCAAGACGGCCGCATCAA
GCTGAACCTGGGCAAGAACCGCTCCATGGACTTCCGCGTCAGCACGCTGCCGACCCTGTTCGGCGAGAAGATCGTGCTGC
GTATTCTGGACAGCGCCTCGGCCAAACTCGGCATCGACAAGCTGGGTTACGAGGAAGACCAGAAGCAGCTTTACCTGGAT
GCCATTGCCAAGCCATACGGCATGGTGCTGGTCACCGGCCCGACCGGTTCCGGCAAGACCGTGTCGCTGTACACGGCGCT
GAACATCCTCAACACGGACGAACGCAACATCTCCACCGTCGAAGACCCGGTCGAAATCCGTGTCGAAGGCATCAACCAGG
TTCAGCAGAACACCAAGCGCGGCATGACCTTCGCCGCCGCGCTGAAATCTTTCCTGCGCCAGGATCCGGACGTGATCATG
GTCGGCGAAATCCGTGACCTGGAAACCGCCGAAATCGCGATCAAGGCCGCCCAGACCGGCCACATGGTGCTGTCCACACT
GCACACCAACGATGCGCCGCAGACCATTGCCCGCCTGATGAACATGGGCATCGCGCCCTACAACATCACGTCCTCGGTCA
CCCTGATCATCGCCCAGCGACTGGCGCGGCGCCTGCACAGCTGCAAGCGCCCGATCGACCTGCCGCCACAGGCGCTGCTC
GACGCCGGCTTCACCCAGGAAGAGATCGATGCCGGCCTGACCGTCTACGAGGCCGTCGGCTGCGATGAGTGCAACGAGGG
CTACAAGGGCCGCGTCGGTATTTACCAGGTGATGCCGATGGCGGAAGAGATCCAGAAGATCGTGCTACAGGGCGGCAACG
CATTGCAGATCGCCGAAGTGGCCAGCCAGGCCGGCATCAACGATCTGCGCGCCTCGGCGCTGAAAAAGGTGAAGGACGGC
GTCACCAGCCTTACCGAGATCGACCGGGTAACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-147)

Type II/IV secretion system protein (T2SSE)

(197-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.282

99.127

0.548
  pilB Acinetobacter baylyi ADP1

54.965

98.429

0.541
  pilB Legionella pneumophila strain ERS1305867

50.791

99.302

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

47.788

98.604

0.471
  pilB Vibrio cholerae strain A1552

50.755

92.496

0.469
  pilB Vibrio campbellii strain DS40M4

48.785

93.368

0.455
  pilF Neisseria gonorrhoeae MS11

49.245

92.496

0.455
  pilF Thermus thermophilus HB27

37.871

100

0.379