Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RAH45_RS00385 Genome accession   NZ_CP132595
Coordinates   89363..90997 (-) Length   544 a.a.
NCBI ID   WP_068407905.1    Uniprot ID   -
Organism   Psychrobacter sp. van23A     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 84363..95997
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAH45_RS00360 (RAH45_00360) - 84753..85088 (+) 336 WP_068407914.1 pyrimidine/purine nucleoside phosphorylase -
  RAH45_RS00365 (RAH45_00365) rlmB 85453..86223 (+) 771 WP_068407911.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  RAH45_RS00370 (RAH45_00370) coaE 86186..86875 (-) 690 WP_068407908.1 dephospho-CoA kinase -
  RAH45_RS00375 (RAH45_00375) pilD 86992..87891 (-) 900 WP_068407907.1 A24 family peptidase Machinery gene
  RAH45_RS00380 (RAH45_00380) - 87917..89140 (-) 1224 Protein_75 type II secretion system F family protein -
  RAH45_RS00385 (RAH45_00385) pilB 89363..90997 (-) 1635 WP_068407905.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RAH45_RS00390 (RAH45_00390) tpiA 91462..92262 (+) 801 WP_082894377.1 triose-phosphate isomerase -
  RAH45_RS00395 (RAH45_00395) secG 92529..92837 (+) 309 WP_025652589.1 preprotein translocase subunit SecG -
  RAH45_RS00410 (RAH45_00410) rimP 93914..94414 (+) 501 WP_068407904.1 ribosome maturation factor RimP -

Sequence

Protein


Download         Length: 544 a.a.        Molecular weight: 60375.49 Da        Isoelectric Point: 5.2340

>NTDB_id=869531 RAH45_RS00385 WP_068407905.1 89363..90997(-) (pilB) [Psychrobacter sp. van23A]
MKTAQTESQQQKMGLVPYLVDNKLANAYQVAQMLSQAFGDPLFDLDALSSDVIPKNLVDEKIVRKFNALPLFKRGQRLFV
ALSDPTRIDAIDAIAFNSRLSIETVVVEEDKLKKRIENLYADTMQSFDSFSDSDLNIGFNESEEDEGETKLSDGVDEAPV
VKFVNKMLVDAIRMGASDLHFEPYEKTYRVRFRVDGVMQKMASPPVQLAGKIAARLKVMSQMDISERRVPQDGRIKLKIS
KDKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIEALGYEPDQKEMFLEALHKPQGMLLITGPTGSGKTVSLYTGINILN
TGETNISTAEDPVEINLEGINQVNVNPKVGLTFANALKSFLRQDPDIVMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNS
APETLTRLRNMGVASFNIATSVNLVIAQRLARRLCKNCKKPINIPRQSLLELGFTDADLDNPDNVIYEPIGCNECREGYK
GRVGIYEVMKVSPDISRIIMEDGNAIDIKDAALKNGFRDLRRSGILKVLQGVTSIQEMMRVTSE

Nucleotide


Download         Length: 1635 bp        

>NTDB_id=869531 RAH45_RS00385 WP_068407905.1 89363..90997(-) (pilB) [Psychrobacter sp. van23A]
ATGAAAACCGCGCAAACAGAATCACAACAGCAGAAAATGGGCTTGGTTCCCTATTTGGTTGACAATAAGCTGGCCAATGC
TTATCAAGTAGCGCAGATGCTATCGCAAGCGTTTGGTGATCCTTTGTTTGATCTTGATGCCTTAAGCTCAGATGTCATTC
CAAAAAACTTGGTCGATGAAAAAATCGTACGTAAGTTCAACGCCCTACCACTGTTTAAACGCGGGCAACGCTTATTTGTG
GCATTGAGTGACCCGACCCGTATCGATGCCATCGACGCTATTGCCTTTAACTCGCGCCTGTCTATCGAGACTGTCGTCGT
TGAAGAAGATAAGCTCAAAAAGCGTATTGAGAATCTGTACGCTGATACCATGCAAAGTTTTGATAGCTTTTCTGACAGCG
ATTTAAATATTGGCTTTAATGAAAGCGAAGAAGACGAAGGCGAAACCAAACTTAGCGACGGCGTCGATGAAGCGCCCGTG
GTGAAGTTTGTTAATAAAATGTTGGTTGATGCCATTCGTATGGGCGCCTCGGACCTACATTTTGAACCTTATGAAAAAAC
CTATCGCGTGCGCTTTCGTGTCGATGGCGTCATGCAAAAAATGGCCAGTCCGCCGGTACAACTTGCGGGTAAAATTGCCG
CGCGTCTAAAAGTCATGTCGCAAATGGACATCTCTGAGCGCCGTGTACCGCAAGATGGTCGTATTAAGCTTAAAATCTCT
AAAGACAAAGCCATCGATTTCCGCGTAAACTCCCTACCTACCTTATTTGGCGAAAAGCTGGTTTTACGTATTTTGGATCC
ATCATCTGCCATGCTTGGTATTGAAGCATTAGGTTATGAGCCCGACCAAAAAGAGATGTTTTTAGAAGCCTTACATAAGC
CTCAAGGTATGCTGCTGATTACTGGTCCTACTGGTTCAGGTAAAACCGTCTCCCTTTATACCGGTATTAATATTTTAAAT
ACTGGCGAGACCAATATCTCAACCGCCGAAGACCCCGTTGAGATTAATCTTGAAGGTATCAATCAGGTCAACGTTAACCC
GAAAGTCGGTTTAACCTTTGCCAATGCGCTCAAATCATTCTTACGTCAAGATCCTGATATCGTCATGGTCGGTGAGATTC
GTGACCTTGAAACGGCTGAGATTGCGATTAAAGCAGCACAAACTGGACATATGGTGCTGTCTACCCTGCATACCAATTCT
GCCCCTGAAACCTTGACGCGTTTGCGCAATATGGGAGTTGCCTCATTTAACATTGCAACGTCAGTGAACTTGGTTATTGC
CCAGCGATTGGCAAGACGTTTATGCAAAAATTGCAAAAAACCCATCAATATACCGCGCCAAAGCTTGCTTGAGCTTGGTT
TTACCGATGCTGATTTAGACAACCCAGACAATGTCATTTATGAACCTATTGGCTGTAATGAATGCCGTGAAGGCTACAAA
GGCCGTGTCGGTATTTATGAAGTGATGAAAGTCAGCCCCGATATTTCGCGCATTATTATGGAAGATGGTAACGCCATAGA
TATCAAAGACGCTGCCCTTAAAAACGGCTTTAGAGATTTACGCCGCTCGGGTATTTTAAAGGTGCTGCAAGGGGTAACCA
GTATTCAAGAAATGATGCGCGTGACTTCTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(166-433)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-123)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

69.526

100

0.7
  pilB Acinetobacter baylyi ADP1

66.239

100

0.664
  pilB Legionella pneumophila strain ERS1305867

54.19

98.713

0.535
  pilF Neisseria gonorrhoeae MS11

51.565

99.816

0.515
  pilB Vibrio parahaemolyticus RIMD 2210633

50.093

98.346

0.493
  pilB Vibrio cholerae strain A1552

50.284

97.243

0.489
  pilB Vibrio campbellii strain DS40M4

49.905

97.243

0.485
  pilF Thermus thermophilus HB27

37.112

100

0.373
  pilB/pilB1 Synechocystis sp. PCC 6803

38.846

95.588

0.371