Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   RAM00_RS02335 Genome accession   NZ_CP132376
Coordinates   559554..560786 (-) Length   410 a.a.
NCBI ID   WP_025329863.1    Uniprot ID   -
Organism   Snodgrassella alvi strain K-FP26     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 554554..565786
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAM00_RS02300 (RAM00_02295) - 555102..555350 (-) 249 WP_025329856.1 BolA family protein -
  RAM00_RS02305 (RAM00_02300) - 555405..556166 (-) 762 WP_078475028.1 ABC transporter permease -
  RAM00_RS02310 (RAM00_02305) - 556163..557083 (-) 921 WP_025329858.1 ABC transporter ATP-binding protein -
  RAM00_RS02315 (RAM00_02310) yacG 557130..557327 (-) 198 WP_078475027.1 DNA gyrase inhibitor YacG -
  RAM00_RS02320 (RAM00_02315) zapD 557327..558079 (-) 753 WP_025329860.1 cell division protein ZapD -
  RAM00_RS02325 (RAM00_02320) coaE 558085..558699 (-) 615 WP_078475026.1 dephospho-CoA kinase -
  RAM00_RS02330 (RAM00_02325) pilD 558696..559547 (-) 852 WP_078475025.1 A24 family peptidase Machinery gene
  RAM00_RS02335 (RAM00_02330) pilG 559554..560786 (-) 1233 WP_025329863.1 type II secretion system F family protein Machinery gene
  RAM00_RS02340 (RAM00_02335) pilF 560835..562514 (-) 1680 WP_037392184.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RAM00_RS02345 (RAM00_02340) recJ 562716..564413 (-) 1698 WP_306176224.1 single-stranded-DNA-specific exonuclease RecJ -
  RAM00_RS02350 (RAM00_02345) - 564410..565384 (-) 975 WP_084585189.1 hypothetical protein -

Sequence

Protein


Download         Length: 410 a.a.        Molecular weight: 45046.94 Da        Isoelectric Point: 9.8445

>NTDB_id=868783 RAM00_RS02335 WP_025329863.1 559554..560786(-) (pilG) [Snodgrassella alvi strain K-FP26]
MNKPVAVSKTKSSGTSFSFEGKNLKTEQIVRGEVVAKDEADARAKLTRRQIRVIQIAKVKKQRQKKISQADITVFTRQLS
TMMKSGLPMMQAFDIAAKGHSNPAMTQLLLQIRSDVEQGTSLAESFSKHPKYFDHFYCNLVHAGEAGGVLEAILDKLSVY
KEKTQGIKKKVKSALTYPAVVVVVAIALVIVMMMFVLPSFGKVYESMGVPLPGLTQFMMNISNFFVHYGIFVIIALIGGV
IGLFKWYQNSDDLKKRFDYMLLKMPIFGQIVRKATIARWSRTTATLFAAGVPLVEVLDSVAGASGNIRYEEATQEIRAKV
NQGISLTSAMQATELFPNMVMQMASIGEESGSLDDMLNKAAEFYEEDVDTAVAQLSSLMEPIIMVVLGGIVGIILVAMYL
PLFNIGNAVG

Nucleotide


Download         Length: 1233 bp        

>NTDB_id=868783 RAM00_RS02335 WP_025329863.1 559554..560786(-) (pilG) [Snodgrassella alvi strain K-FP26]
ATGAATAAACCCGTAGCAGTATCCAAAACCAAATCTTCCGGTACCAGTTTCAGTTTTGAAGGCAAAAACCTTAAAACTGA
ACAGATTGTACGCGGAGAAGTTGTCGCAAAAGACGAGGCTGATGCACGCGCCAAGCTAACCCGGCGTCAAATTCGTGTAA
TACAGATTGCCAAGGTCAAAAAACAGCGCCAAAAGAAAATCAGTCAGGCAGATATTACCGTCTTTACCCGACAATTATCC
ACCATGATGAAATCTGGTCTGCCTATGATGCAGGCTTTCGATATTGCAGCCAAAGGGCATTCTAACCCGGCAATGACCCA
GCTACTGTTGCAAATCCGCTCTGATGTTGAGCAAGGTACATCTCTGGCAGAGTCTTTTTCTAAACATCCGAAATATTTCG
ATCATTTTTACTGTAATCTGGTGCATGCGGGTGAAGCCGGTGGTGTACTAGAGGCTATTCTTGACAAGCTGTCGGTATAT
AAAGAAAAAACTCAGGGTATCAAGAAAAAAGTTAAAAGTGCCTTGACCTACCCAGCTGTGGTAGTAGTCGTAGCAATCGC
ACTGGTAATCGTCATGATGATGTTCGTATTACCTTCATTTGGTAAAGTTTACGAGAGTATGGGCGTACCATTACCTGGTT
TGACTCAGTTCATGATGAATATTTCCAATTTCTTTGTACATTACGGCATATTTGTCATCATTGCACTGATAGGTGGTGTA
ATTGGCTTATTTAAGTGGTATCAGAACTCAGATGATCTGAAAAAACGTTTTGATTATATGCTGTTAAAAATGCCTATTTT
CGGCCAAATCGTTCGTAAAGCCACCATTGCACGCTGGTCTCGTACAACAGCAACTCTATTTGCAGCAGGCGTACCATTGG
TAGAAGTTCTTGATTCAGTTGCCGGAGCTTCCGGTAATATTCGCTATGAAGAAGCTACTCAGGAAATCCGTGCCAAAGTT
AATCAGGGTATTTCTCTTACCTCAGCCATGCAGGCAACAGAGTTGTTTCCGAACATGGTGATGCAAATGGCTTCAATCGG
TGAAGAATCAGGTTCTTTGGATGACATGCTCAATAAAGCCGCAGAATTCTATGAAGAAGATGTAGATACTGCCGTTGCAC
AACTGTCTTCATTGATGGAACCGATTATTATGGTTGTACTTGGTGGTATTGTCGGGATAATTCTGGTTGCCATGTATTTA
CCGCTATTTAATATCGGTAATGCTGTAGGTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(75-198)

Type II secretion system (T2SS), protein F (T2SSF)

(279-401)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

64.885

95.854

0.622
  pilG Neisseria meningitidis 44/76-A

64.122

95.854

0.615
  pilC Legionella pneumophila strain ERS1305867

48.12

97.317

0.468
  pilC Acinetobacter baylyi ADP1

44.361

97.317

0.432
  pilC Acinetobacter baumannii D1279779

44.361

97.317

0.432
  pilC Pseudomonas stutzeri DSM 10701

41.855

97.317

0.407
  pilC Vibrio cholerae strain A1552

38.06

98.049

0.373