Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   RAM05_RS02620 Genome accession   NZ_CP132375
Coordinates   602234..603466 (-) Length   410 a.a.
NCBI ID   WP_025329863.1    Uniprot ID   -
Organism   Snodgrassella alvi strain wkB9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 597234..608466
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAM05_RS02585 (RAM05_02585) - 597782..598030 (-) 249 WP_025329856.1 BolA family protein -
  RAM05_RS02590 (RAM05_02590) - 598085..598846 (-) 762 WP_038648602.1 ABC transporter permease -
  RAM05_RS02595 (RAM05_02595) - 598843..599763 (-) 921 WP_025329858.1 ABC transporter ATP-binding protein -
  RAM05_RS02600 (RAM05_02600) yacG 599810..600007 (-) 198 WP_025329859.1 DNA gyrase inhibitor YacG -
  RAM05_RS02605 (RAM05_02605) zapD 600007..600759 (-) 753 WP_025329860.1 cell division protein ZapD -
  RAM05_RS02610 (RAM05_02610) coaE 600765..601379 (-) 615 WP_025329861.1 dephospho-CoA kinase -
  RAM05_RS02615 (RAM05_02615) pilD 601376..602227 (-) 852 WP_025329862.1 A24 family peptidase Machinery gene
  RAM05_RS02620 (RAM05_02620) pilG 602234..603466 (-) 1233 WP_025329863.1 type II secretion system F family protein Machinery gene
  RAM05_RS02625 (RAM05_02625) pilF 603515..605194 (-) 1680 WP_025329864.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RAM05_RS02630 (RAM05_02630) recJ 605396..607093 (-) 1698 WP_025329865.1 single-stranded-DNA-specific exonuclease RecJ -
  RAM05_RS02635 (RAM05_02635) - 607090..608064 (-) 975 WP_025329866.1 hypothetical protein -

Sequence

Protein


Download         Length: 410 a.a.        Molecular weight: 45046.94 Da        Isoelectric Point: 9.8445

>NTDB_id=868759 RAM05_RS02620 WP_025329863.1 602234..603466(-) (pilG) [Snodgrassella alvi strain wkB9]
MNKPVAVSKTKSSGTSFSFEGKNLKTEQIVRGEVVAKDEADARAKLTRRQIRVIQIAKVKKQRQKKISQADITVFTRQLS
TMMKSGLPMMQAFDIAAKGHSNPAMTQLLLQIRSDVEQGTSLAESFSKHPKYFDHFYCNLVHAGEAGGVLEAILDKLSVY
KEKTQGIKKKVKSALTYPAVVVVVAIALVIVMMMFVLPSFGKVYESMGVPLPGLTQFMMNISNFFVHYGIFVIIALIGGV
IGLFKWYQNSDDLKKRFDYMLLKMPIFGQIVRKATIARWSRTTATLFAAGVPLVEVLDSVAGASGNIRYEEATQEIRAKV
NQGISLTSAMQATELFPNMVMQMASIGEESGSLDDMLNKAAEFYEEDVDTAVAQLSSLMEPIIMVVLGGIVGIILVAMYL
PLFNIGNAVG

Nucleotide


Download         Length: 1233 bp        

>NTDB_id=868759 RAM05_RS02620 WP_025329863.1 602234..603466(-) (pilG) [Snodgrassella alvi strain wkB9]
ATGAATAAACCCGTAGCAGTATCCAAAACCAAATCCTCCGGAACCAGTTTCAGTTTTGAAGGCAAAAACCTTAAAACTGA
ACAGATTGTACGCGGAGAAGTTGTCGCGAAAGACGAGGCTGATGCACGCGCCAAGCTAACCCGGCGTCAAATTCGTGTAA
TACAGATTGCCAAGGTCAAAAAACAGCGCCAAAAGAAAATTAGTCAGGCAGATATTACCGTCTTTACCCGGCAGTTATCC
ACCATGATGAAATCTGGTCTGCCTATGATGCAGGCTTTCGATATTGCAGCCAAAGGCCATTCCAACCCGGCAATGACCCA
GCTACTGTTGCAAATCCGCTCTGATGTTGAGCAAGGTACATCTCTGGCAGAGTCTTTTTCTAAACATCCGAAATATTTCG
ATCATTTTTACTGTAATCTGGTGCATGCGGGTGAAGCCGGTGGTGTACTAGAGGCTATTCTTGACAAGCTGTCGGTATAT
AAAGAAAAAACTCAGGGTATCAAGAAAAAAGTTAAAAGTGCCTTGACCTACCCAGCTGTGGTAGTAGTCGTAGCAATCGC
ACTGGTAATCGTCATGATGATGTTCGTATTACCTTCATTTGGTAAAGTTTACGAGAGTATGGGCGTACCATTACCTGGTT
TGACTCAGTTCATGATGAATATTTCCAATTTCTTTGTACATTACGGCATATTTGTCATCATTGCACTGATAGGTGGTGTA
ATTGGCTTATTTAAGTGGTATCAGAACTCAGATGATCTGAAAAAACGTTTTGATTATATGCTGTTAAAAATGCCTATTTT
CGGCCAAATCGTTCGTAAAGCCACCATTGCACGCTGGTCTCGTACAACAGCAACTCTATTTGCAGCAGGCGTACCATTGG
TAGAAGTTCTTGATTCAGTTGCCGGAGCTTCCGGTAATATTCGCTATGAAGAAGCTACTCAGGAAATCCGTGCCAAAGTT
AATCAGGGTATTTCTCTTACCTCAGCCATGCAGGCAACAGAGTTGTTTCCGAACATGGTGATGCAAATGGCTTCAATCGG
TGAAGAATCAGGTTCTTTGGATGACATGCTCAATAAAGCCGCAGAATTCTATGAAGAAGATGTAGATACTGCCGTTGCAC
AACTGTCTTCATTGATGGAACCGATTATTATGGTTGTACTTGGTGGTATTGTCGGGATAATTCTGGTTGCCATGTATTTA
CCGCTATTTAATATCGGTAATGCTGTAGGTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(75-198)

Type II secretion system (T2SS), protein F (T2SSF)

(279-401)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

64.885

95.854

0.622
  pilG Neisseria meningitidis 44/76-A

64.122

95.854

0.615
  pilC Legionella pneumophila strain ERS1305867

48.12

97.317

0.468
  pilC Acinetobacter baylyi ADP1

44.361

97.317

0.432
  pilC Acinetobacter baumannii D1279779

44.361

97.317

0.432
  pilC Pseudomonas stutzeri DSM 10701

41.855

97.317

0.407
  pilC Vibrio cholerae strain A1552

38.06

98.049

0.373