Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   RAM23_RS02385 Genome accession   NZ_CP132374
Coordinates   565382..566614 (-) Length   410 a.a.
NCBI ID   WP_100125701.1    Uniprot ID   -
Organism   Snodgrassella alvi strain wkB339     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 560382..571614
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAM23_RS02350 (RAM23_02345) - 560930..561178 (-) 249 WP_025329856.1 BolA family protein -
  RAM23_RS02355 (RAM23_02350) - 561233..561994 (-) 762 WP_078475028.1 ABC transporter permease -
  RAM23_RS02360 (RAM23_02355) - 561991..562911 (-) 921 WP_025329858.1 ABC transporter ATP-binding protein -
  RAM23_RS02365 (RAM23_02360) yacG 562958..563155 (-) 198 WP_078475027.1 DNA gyrase inhibitor YacG -
  RAM23_RS02370 (RAM23_02365) zapD 563155..563907 (-) 753 WP_025329860.1 cell division protein ZapD -
  RAM23_RS02375 (RAM23_02370) coaE 563913..564527 (-) 615 WP_078475026.1 dephospho-CoA kinase -
  RAM23_RS02380 (RAM23_02375) pilD 564524..565375 (-) 852 WP_078475025.1 A24 family peptidase Machinery gene
  RAM23_RS02385 (RAM23_02380) pilG 565382..566614 (-) 1233 WP_100125701.1 type II secretion system F family protein Machinery gene
  RAM23_RS02390 (RAM23_02385) pilF 566663..568342 (-) 1680 WP_100125700.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RAM23_RS02395 (RAM23_02390) recJ 568543..570240 (-) 1698 WP_100125699.1 single-stranded-DNA-specific exonuclease RecJ -
  RAM23_RS02400 (RAM23_02395) - 570237..571211 (-) 975 WP_100125698.1 hypothetical protein -

Sequence

Protein


Download         Length: 410 a.a.        Molecular weight: 45079.00 Da        Isoelectric Point: 9.8445

>NTDB_id=868733 RAM23_RS02385 WP_100125701.1 565382..566614(-) (pilG) [Snodgrassella alvi strain wkB339]
MNKPVAVSKTKSTGTSFSFEGKNLKTEQIVRGEVVAKDEADARAKLTRRQIRVIQIAKVKKQRQKKISQADITVFTRQLS
TMMKSGLPMMQAFDIAAKGHSNPAMTQLLMQIRSDVEQGTSLAESFSKHPKYFDHFYCNLVHAGEAGGVLEAILDKLSVY
KEKTQGIKKKVKSALTYPAVVVVVAIALVIVMMMFVLPSFGKVYESMGVPLPGLTQFMMNISNFFVHYGIFVIIALIGGV
IGLFKWYQNSDDLKKRFDYMLLKMPIFGQIVRKATIARWSRTTATLFAAGVPLVEVLDSVAGASGNIRYEEATQEIRAKV
NQGISLTSAMQATELFPNMVMQMASIGEESGSLDDMLNKAAEFYEEDVDTAVAQLSSLMEPIIMVVLGGIVGIILVAMYL
PLFNIGNAVG

Nucleotide


Download         Length: 1233 bp        

>NTDB_id=868733 RAM23_RS02385 WP_100125701.1 565382..566614(-) (pilG) [Snodgrassella alvi strain wkB339]
ATGAATAAACCCGTAGCAGTATCCAAAACCAAATCCACCGGAACCAGTTTCAGTTTTGAAGGCAAAAACCTTAAAACAGA
ACAGATTGTACGCGGAGAAGTTGTCGCGAAAGACGAGGCTGATGCACGCGCCAAGCTAACCCGGCGTCAAATTCGTGTAA
TACAGATTGCCAAGGTCAAAAAACAGCGCCAAAAGAAAATCAGTCAGGCAGATATTACCGTTTTTACCCGGCAATTATCC
ACCATGATGAAATCCGGTCTACCTATGATGCAGGCTTTCGATATTGCAGCCAAAGGTCATTCTAACCCGGCAATGACCCA
GCTATTGATGCAAATCCGCTCTGATGTTGAGCAAGGTACATCTCTGGCAGAGTCTTTTTCTAAACATCCGAAATATTTCG
ATCATTTTTACTGTAATCTGGTGCATGCGGGTGAAGCCGGTGGTGTACTAGAGGCTATTCTTGACAAGCTGTCGGTATAT
AAAGAAAAAACTCAGGGTATCAAGAAAAAAGTTAAAAGTGCCTTGACCTACCCAGCTGTGGTAGTAGTCGTAGCAATCGC
ACTGGTAATCGTCATGATGATGTTCGTATTACCTTCATTTGGTAAAGTTTACGAGAGTATGGGCGTACCATTACCTGGTT
TGACTCAGTTCATGATGAATATTTCCAATTTCTTTGTACATTACGGCATATTTGTCATCATTGCACTGATAGGTGGTGTA
ATTGGCTTATTTAAGTGGTATCAGAACTCAGATGATCTGAAAAAACGTTTTGATTATATGCTGTTAAAAATGCCTATTTT
CGGCCAAATCGTTCGTAAAGCCACCATTGCACGCTGGTCTCGTACAACAGCAACTCTATTTGCAGCAGGCGTACCATTGG
TAGAAGTTCTTGATTCAGTTGCCGGAGCTTCCGGTAATATTCGCTATGAAGAAGCTACTCAGGAAATCCGTGCCAAAGTT
AATCAGGGTATTTCTCTTACCTCAGCCATGCAGGCAACAGAGTTGTTTCCGAACATGGTGATGCAAATGGCTTCAATCGG
TGAAGAATCAGGTTCTTTGGATGACATGCTCAATAAAGCCGCAGAATTCTATGAAGAAGATGTAGATACTGCCGTTGCAC
AACTGTCTTCATTGATGGAACCGATTATTATGGTTGTACTTGGTGGTATTGTCGGGATAATTCTGGTTGCCATGTATTTA
CCGCTATTTAATATCGGTAATGCTGTAGGTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(75-198)

Type II secretion system (T2SS), protein F (T2SSF)

(279-401)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

65.14

95.854

0.624
  pilG Neisseria meningitidis 44/76-A

64.377

95.854

0.617
  pilC Legionella pneumophila strain ERS1305867

48.12

97.317

0.468
  pilC Acinetobacter baylyi ADP1

44.11

97.317

0.429
  pilC Acinetobacter baumannii D1279779

44.11

97.317

0.429
  pilC Pseudomonas stutzeri DSM 10701

41.855

97.317

0.407
  pilC Vibrio cholerae strain A1552

38.06

98.049

0.373