Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RAB70_RS09065 Genome accession   NZ_CP132342
Coordinates   2141428..2143161 (+) Length   577 a.a.
NCBI ID   WP_148828469.1    Uniprot ID   -
Organism   Xanthomonas sontii strain PPL1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2136428..2148161
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAB70_RS09045 (RAB70_09045) - 2137507..2138640 (-) 1134 WP_148828473.1 glycosyltransferase -
  RAB70_RS09050 (RAB70_09050) - 2138682..2139632 (-) 951 WP_148828472.1 YbhN family protein -
  RAB70_RS09055 (RAB70_09055) - 2139629..2140417 (-) 789 WP_148828471.1 bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG -
  RAB70_RS09060 (RAB70_09060) - 2140440..2141297 (-) 858 WP_148828470.1 glycosyltransferase family 2 protein -
  RAB70_RS09065 (RAB70_09065) pilB 2141428..2143161 (+) 1734 WP_148828469.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RAB70_RS09070 (RAB70_09070) pilR 2143378..2144766 (-) 1389 WP_148828468.1 sigma-54 dependent transcriptional regulator Regulator
  RAB70_RS09075 (RAB70_09075) - 2144849..2146462 (-) 1614 WP_148828467.1 PAS domain-containing sensor histidine kinase -
  RAB70_RS09080 (RAB70_09080) sucC 2146854..2148023 (+) 1170 WP_010342227.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62597.87 Da        Isoelectric Point: 5.6425

>NTDB_id=868275 RAB70_RS09065 WP_148828469.1 2141428..2143161(+) (pilB) [Xanthomonas sontii strain PPL1]
MNVAVSANLVGITGIARRLVQDGALDEAAARTAMAQAGEAKVPLPQWFSEKKLVTAAQLAAANAVEFGMPLMDVSVFDAS
QNAMKLVSEELLQKYQVLPLFKRGGRLFVGISNPTQTRALDDIKFHTNLTVEPILVDEDQIRRTLEQWQASNDSLGSALG
DDEGMDNLDVGGGDEDMGGGGDTGIDAKGEDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKSVAKAPV
KLNQRIAARLKVMAQLDIAEKRIPQDGRIKLNLSKNKQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIDKLGYEPEQQKL
FLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDDTRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
IIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRPVQLPE
KALLAEGFTEAEIAAGVQLYEAVGCDECTEGYKGRTGIYQVMPMNDEIAAIVLQGGNAMDIASAAQKIGVKDLRQSALLK
ARNGITSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=868275 RAB70_RS09065 WP_148828469.1 2141428..2143161(+) (pilB) [Xanthomonas sontii strain PPL1]
ATGAACGTAGCTGTATCGGCCAACCTTGTCGGCATCACCGGGATCGCCCGCCGCCTCGTGCAGGACGGCGCGCTGGACGA
AGCCGCAGCGCGGACTGCCATGGCCCAGGCGGGCGAGGCCAAGGTGCCGCTGCCGCAGTGGTTCTCCGAGAAGAAGCTGG
TCACCGCCGCGCAGCTGGCCGCCGCCAATGCGGTGGAGTTCGGCATGCCGTTGATGGATGTCTCGGTCTTCGATGCCAGC
CAGAACGCGATGAAGCTGGTCAGCGAGGAGTTGCTGCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGTGGCCGCCT
GTTCGTCGGCATCAGCAACCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTCCACACCAATCTGACGGTGGAGCCGA
TCCTGGTCGACGAGGACCAGATCCGCCGGACCCTGGAGCAGTGGCAGGCCAGCAACGATTCCCTCGGCAGCGCGCTGGGC
GACGACGAGGGCATGGACAACCTCGATGTCGGCGGCGGCGACGAGGACATGGGCGGCGGTGGCGACACCGGCATCGACGC
CAAGGGGGAGGACACCCCGGTCGTCAAGTTCGTGAACAAGGTGCTGGTGGATGCGATCCGCCGCGGCGCGTCCGACATCC
ACTTCGAGCCCTACGAGGACGACTACCGGGTGCGCCTGCGCATCGATGGCCTGCTCAAGAGCGTGGCCAAGGCGCCGGTC
AAGCTCAACCAGCGCATCGCCGCGCGCCTGAAGGTGATGGCGCAGCTGGACATCGCCGAGAAGCGGATCCCGCAGGACGG
CCGCATCAAGCTCAATCTGTCCAAGAACAAGCAGATCGATTTCCGCGTCAGCACCCTGCCCACGCTGTTCGGCGAGAAGA
TCGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGTATCGACAAGCTCGGCTACGAGCCGGAGCAGCAGAAGCTG
TTCCTGGACGCGATCCACAAGCCGTACGGCATGGTCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTGTCGCTGTA
CACCGCGCTGGGCATCCTCAACGACGACACCCGCAACATCTCCACCGCCGAGGATCCGGTCGAAATCCGCCTGCCCGGGG
TCAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCGGCGGCGCTGCGCTCGTTCCTGCGCCAGGATCCGGAC
ATCATCATGGTCGGCGAAATCCGCGACCTGGAGACCGCCGAGATCGCGATCAAGGCCGCGCAGACCGGTCACATGGTGCT
GTCCACCCTGCACACCAACGACGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCGCCCTACAACATCACCA
GTTCGGTGACCCTGGTCATCGCCCAGCGCCTGGCGCGGCGCCTGTGCAACAACTGCAAGCGTCCGGTCCAGTTGCCGGAA
AAGGCCTTGCTGGCCGAAGGCTTCACCGAGGCGGAGATCGCCGCCGGCGTCCAACTGTACGAGGCGGTGGGCTGCGACGA
GTGCACCGAGGGCTACAAGGGCCGCACCGGCATCTATCAGGTGATGCCGATGAACGACGAGATCGCGGCGATCGTGCTGC
AGGGCGGCAACGCCATGGACATCGCCAGCGCCGCGCAGAAGATCGGGGTCAAGGACCTGCGCCAGTCGGCCCTGCTCAAG
GCCCGCAACGGCATCACCAGCCTGGCCGAGATCAATCGGGTGACGAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.737

98.787

0.541
  pilB Acinetobacter baylyi ADP1

54.545

99.133

0.541
  pilB Legionella pneumophila strain ERS1305867

51.318

98.614

0.506
  pilB Vibrio cholerae strain A1552

49.913

99.307

0.496
  pilF Neisseria gonorrhoeae MS11

49.472

98.44

0.487
  pilB Vibrio parahaemolyticus RIMD 2210633

46.046

98.614

0.454
  pilB Vibrio campbellii strain DS40M4

45.694

98.614

0.451
  pilB/pilB1 Synechocystis sp. PCC 6803

36.721

100

0.388
  pilF Thermus thermophilus HB27

39.619

90.988

0.36