Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   RAL92_RS25645 Genome accession   NZ_CP132337
Coordinates   5532704..5534794 (-) Length   696 a.a.
NCBI ID   WP_346841202.1    Uniprot ID   -
Organism   Pseudomonas otitidis strain Tg_9B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5527704..5539794
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAL92_RS25630 (RAL92_25630) - 5529346..5531013 (-) 1668 WP_346841200.1 SPOR domain-containing protein -
  RAL92_RS25635 (RAL92_25635) aroB 5531024..5532127 (-) 1104 WP_346841201.1 3-dehydroquinate synthase -
  RAL92_RS25640 (RAL92_25640) aroK 5532180..5532698 (-) 519 WP_074973697.1 shikimate kinase AroK -
  RAL92_RS25645 (RAL92_25645) pilQ 5532704..5534794 (-) 2091 WP_346841202.1 type IV pilus secretin PilQ Machinery gene
  RAL92_RS25650 (RAL92_25650) pilP 5534849..5535379 (-) 531 WP_346842619.1 type 4a pilus biogenesis lipoprotein PilP -
  RAL92_RS25655 (RAL92_25655) pilO 5535379..5536002 (-) 624 WP_044406732.1 type 4a pilus biogenesis protein PilO -
  RAL92_RS25660 (RAL92_25660) pilN 5535999..5536571 (-) 573 WP_044406731.1 type 4a pilus biogenesis protein PilN -
  RAL92_RS25665 (RAL92_25665) pilM 5536571..5537635 (-) 1065 WP_044406728.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 696 a.a.        Molecular weight: 74787.04 Da        Isoelectric Point: 5.1117

>NTDB_id=868226 RAL92_RS25645 WP_346841202.1 5532704..5534794(-) (pilQ) [Pseudomonas otitidis strain Tg_9B]
MNSSLTRLGLSLLAAMFSPMLLAADLKALDVAALPGGKVELKLGFDGPVVQPRGYTIEQPARIALDLPGVANKLGSKNRE
LGVGNARSVTLVEAKDRTRMIINLSTLVPYSTRVEGNNLYVLVGDAAGAAQQAARASAPVAAPSQPAAPVKAFAPAGKAI
SNIDFQRGEQGEGNVVITLSDPSVSPNIQEQGGKIRLDFSKTQIPESLRVRLDVKDFATPVQFVSASGSSGDASIVIEPT
GFYDYLAYQTENKLTVSIKPLTQEDMEKRKTERFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLRLQ
NVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQISELAPLRRELIQVNYAKAADIAKLFQSVTSGE
SSSTDERGSITVDDRTNSIIAYQTQDKLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVKWRGARVGDNNFVVGG
GAQATGANRIGATTPSGALGTFVDMGATNATSSIGIGFITDNTILDLELSAMEKTGNGEIVSQPKVVTSDKETAKILKGS
EIPYQEASSSGATSVSFKEAALSLEVTPQITPDNRVLMEVKVTKDAPDFSRQVNGVPPINKNEVNAKVLVSDGETIVIGG
VFSNTQTKSTDKVPFLGDLPYLGRMFKRDVVQDNKAELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2091 bp        

>NTDB_id=868226 RAL92_RS25645 WP_346841202.1 5532704..5534794(-) (pilQ) [Pseudomonas otitidis strain Tg_9B]
ATGAACAGCTCCCTAACGCGCCTTGGACTTTCTCTTCTGGCGGCCATGTTTTCACCCATGTTGCTGGCTGCTGACCTCAA
GGCTCTGGACGTCGCGGCCCTGCCGGGCGGAAAGGTCGAGCTGAAACTCGGTTTCGACGGCCCCGTTGTACAGCCGAGAG
GCTACACGATTGAGCAGCCTGCAAGGATCGCATTGGACCTGCCTGGCGTTGCCAACAAACTTGGCAGCAAGAACCGCGAA
CTAGGGGTAGGTAACGCTCGGAGCGTTACTCTTGTCGAGGCGAAAGATCGCACTCGCATGATCATCAACCTGTCGACGTT
GGTGCCTTACAGCACGCGAGTTGAGGGTAACAACCTTTATGTTCTGGTTGGCGATGCTGCCGGTGCTGCTCAACAGGCTG
CACGCGCCAGTGCGCCGGTAGCGGCTCCGTCACAGCCTGCCGCACCTGTCAAAGCGTTCGCGCCTGCGGGCAAGGCAATC
AGCAACATTGATTTCCAGAGGGGCGAGCAGGGCGAGGGTAACGTCGTCATTACCCTATCCGACCCCAGTGTGAGTCCCAA
CATCCAGGAACAGGGTGGAAAGATTCGCCTGGACTTTTCCAAGACCCAAATTCCGGAGTCTCTGCGTGTTCGTCTGGATG
TAAAGGATTTCGCGACCCCGGTTCAGTTCGTCAGTGCCTCTGGCAGCTCGGGTGATGCCAGTATCGTGATCGAGCCGACG
GGCTTCTACGACTACCTGGCCTACCAGACTGAAAACAAGCTGACTGTCAGTATCAAACCGCTGACCCAAGAAGATATGGA
GAAGCGCAAGACCGAGCGCTTTGCTTATTCTGGCGAAAAGCTCTCGCTCAATTTCCAGGATATTGATGTCCGCTCGGTAC
TGCAGCTGATCGCTGACTTCACCGATCTGAACCTGGTTGCGTCCGATACTGTACAAGGCAACATCACGCTCCGCCTGCAG
AACGTACCTTGGGATCAGGCACTTGACCTGGTCCTGAAAACCAAGGGACTGGACAAGCGCAAGGTCGGTAACGTGCTGCT
GGTTGCACCAGCGGACGAGATCGCTGCCCGTGAACGCCAAGAGCTGGAGTCTCAGAAGCAGATTTCCGAATTGGCTCCGC
TGCGCCGCGAGCTTATCCAGGTCAACTACGCCAAGGCGGCAGACATCGCTAAGCTCTTCCAGTCTGTGACTAGCGGTGAG
TCTTCTTCTACGGATGAGCGCGGCTCCATAACGGTCGATGATCGGACGAACAGCATCATCGCTTATCAAACCCAAGATAA
GCTCGACGAACTTCGTCGTATTGTTGCTCAGCTCGATATTCCGGTCCGTCAGGTGATGATTGAGGCGCGAATAGTTGAAG
CTAATGTTGATTACGATAAGTCGCTCGGAGTCAAGTGGCGTGGGGCGCGAGTCGGTGATAATAACTTTGTAGTGGGTGGT
GGGGCTCAGGCGACCGGTGCTAATCGCATTGGCGCTACTACCCCGAGCGGCGCCCTCGGCACCTTCGTTGATATGGGGGC
AACCAACGCAACCTCATCTATCGGGATCGGTTTCATCACCGATAACACCATCCTGGATCTGGAGTTGTCCGCTATGGAAA
AAACGGGCAATGGCGAGATCGTGTCTCAGCCGAAGGTCGTGACCTCCGATAAGGAGACTGCAAAGATTCTGAAAGGTTCC
GAGATTCCCTATCAGGAAGCCAGCTCGAGTGGTGCAACGTCTGTTTCATTTAAGGAGGCTGCGCTGTCCTTGGAAGTTAC
ACCTCAGATTACTCCAGATAATAGGGTCTTGATGGAGGTGAAGGTTACTAAGGATGCTCCTGACTTCTCTCGCCAGGTTA
ATGGCGTTCCGCCAATCAACAAGAATGAAGTTAACGCAAAGGTCTTGGTAAGCGATGGCGAAACGATTGTGATTGGTGGC
GTATTCTCGAATACTCAGACCAAATCTACCGACAAGGTCCCATTCCTTGGTGATTTGCCCTACCTCGGTCGTATGTTCAA
AAGAGATGTTGTTCAGGACAACAAAGCCGAGCTGCTGGTATTCCTGACTCCGCGTATCATGAACAACCAGGCTATCGCTG
TGAGTCGCTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(378-444)

AMIN domain (AMIN)

(40-121)

Secretin and TonB N terminus short domain (STN)

(304-350)

Bacterial type II and III secretion system protein (Secretin)

(531-687)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

75.978

100

0.782
  comQ Acinetobacter baylyi ADP1

42.981

99.282

0.427
  pilQ Acinetobacter baumannii strain A118

41.292

100

0.422
  pilQ Acinetobacter baumannii D1279779

41.292

100

0.422
  pilQ Legionella pneumophila strain ERS1305867

38.791

97.414

0.378
  pilQ Legionella pneumophila strain Lp02

38.791

97.414

0.378