Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   RAL92_RS25665 Genome accession   NZ_CP132337
Coordinates   5536571..5537635 (-) Length   354 a.a.
NCBI ID   WP_044406728.1    Uniprot ID   A0A0D6SQX4
Organism   Pseudomonas otitidis strain Tg_9B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5531571..5542635
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAL92_RS25640 (RAL92_25640) aroK 5532180..5532698 (-) 519 WP_074973697.1 shikimate kinase AroK -
  RAL92_RS25645 (RAL92_25645) pilQ 5532704..5534794 (-) 2091 WP_346841202.1 type IV pilus secretin PilQ Machinery gene
  RAL92_RS25650 (RAL92_25650) pilP 5534849..5535379 (-) 531 WP_346842619.1 type 4a pilus biogenesis lipoprotein PilP -
  RAL92_RS25655 (RAL92_25655) pilO 5535379..5536002 (-) 624 WP_044406732.1 type 4a pilus biogenesis protein PilO -
  RAL92_RS25660 (RAL92_25660) pilN 5535999..5536571 (-) 573 WP_044406731.1 type 4a pilus biogenesis protein PilN -
  RAL92_RS25665 (RAL92_25665) pilM 5536571..5537635 (-) 1065 WP_044406728.1 pilus assembly protein PilM Machinery gene
  RAL92_RS25670 (RAL92_25670) - 5537820..5540240 (+) 2421 WP_160481841.1 penicillin-binding protein 1A -
  RAL92_RS25675 (RAL92_25675) - 5540346..5541614 (-) 1269 WP_346841203.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37701.00 Da        Isoelectric Point: 4.3980

>NTDB_id=868227 RAL92_RS25665 WP_044406728.1 5536571..5537635(-) (pilM) [Pseudomonas otitidis strain Tg_9B]
MLGLFNKKANSLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALSRVVAKAKAGSKLAAV
AVAGSAVITKTIEMDAGLSDDELENQLKIEADQYIPYPLEEVAIDFEVQGVSARNPERMDVLLAACRRENVEVREAALAL
AGLTAKVVDVEAYALERAYGLLASQLGNGSEDLTVAIVDIGATMTTLSVLNNGRTIYSREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKEAVVQQVSRSLQFFFAAGQYNDVDYILLAGGTASIPDLDRLIQQKIGTPTLVANP
FADMALSGKVNAGALASDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=868227 RAL92_RS25665 WP_044406728.1 5536571..5537635(-) (pilM) [Pseudomonas otitidis strain Tg_9B]
GTGCTAGGGCTCTTCAACAAGAAAGCGAATTCGCTGCTGGGGATCGACATAAGCTCGACCTCGGTCAAGCTCCTCGAATT
GAGTCGCTCGGGTGGCCGCTACAAGGTAGAGGCCTACGCCGTCGAGCCGCTCCCGCCAAATGCTGTCGTCGAAAAGAACA
TTGCTGAGCTGGAAGGCGTCGGCCAGGCACTGTCGCGTGTGGTCGCCAAGGCGAAGGCAGGCTCCAAGCTGGCAGCCGTT
GCTGTGGCGGGGTCGGCCGTCATCACCAAGACGATCGAGATGGATGCGGGGCTGTCCGATGACGAGCTGGAGAACCAGCT
CAAGATCGAAGCTGACCAGTACATTCCCTATCCCCTCGAAGAAGTAGCCATCGATTTCGAGGTTCAGGGCGTTTCTGCAA
GAAACCCCGAGCGAATGGATGTGCTGCTGGCAGCCTGCCGTCGCGAGAACGTCGAGGTGCGCGAGGCAGCGCTTGCTCTT
GCTGGACTCACCGCGAAGGTCGTGGATGTCGAGGCCTATGCGCTTGAGCGTGCCTATGGGCTCCTTGCTTCCCAGTTGGG
GAATGGCTCGGAGGATCTGACGGTTGCGATCGTGGACATCGGCGCGACGATGACCACCCTGAGCGTCTTGAATAATGGTC
GCACCATATACAGTCGCGAGCAGTTGTTCGGAGGTCGCCAGTTGACCGAAGAAATTCAGCGCCGCTATGGCCTTTCTGTC
GAGGAAGCGGGTTTGGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAGGTTTTGCAACCGTTCAAAGAGGC
AGTTGTCCAGCAAGTCTCACGATCGCTGCAGTTCTTTTTCGCAGCGGGCCAGTACAACGATGTCGATTACATCCTCTTGG
CTGGCGGGACGGCCTCTATCCCTGATCTGGATCGCCTGATCCAGCAGAAGATCGGAACGCCGACGCTGGTCGCAAACCCC
TTCGCCGACATGGCACTGAGCGGCAAAGTCAACGCAGGTGCGCTGGCAAGTGATGCGCCGGCCTTGATGATCGCTTGTGG
CTTGGCAATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D6SQX4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

57.062

100

0.571
  comM Acinetobacter nosocomialis M2

57.062

100

0.571
  comM Acinetobacter baylyi ADP1

54.237

100

0.542
  pilM Legionella pneumophila strain ERS1305867

48.023

100

0.48