Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RAL92_RS05590 Genome accession   NZ_CP132337
Coordinates   1186534..1188237 (-) Length   567 a.a.
NCBI ID   WP_346841810.1    Uniprot ID   -
Organism   Pseudomonas otitidis strain Tg_9B     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1181534..1193237
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAL92_RS05550 (RAL92_05555) - 1181716..1182141 (+) 426 WP_044406849.1 GNAT family N-acetyltransferase -
  RAL92_RS05555 (RAL92_05560) - 1182138..1182614 (+) 477 WP_052807598.1 hypothetical protein -
  RAL92_RS05560 (RAL92_05565) - 1182611..1183297 (+) 687 WP_142011982.1 energy-coupling factor ABC transporter permease -
  RAL92_RS05565 (RAL92_05570) - 1183408..1183623 (+) 216 WP_160481384.1 hypothetical protein -
  RAL92_RS05570 (RAL92_05575) yacG 1183636..1183833 (-) 198 WP_044406857.1 DNA gyrase inhibitor YacG -
  RAL92_RS05575 (RAL92_05580) coaE 1183830..1184441 (-) 612 WP_346841808.1 dephospho-CoA kinase -
  RAL92_RS05580 (RAL92_05585) pilD 1184438..1185310 (-) 873 WP_253123383.1 A24 family peptidase Machinery gene
  RAL92_RS05585 (RAL92_05590) pilC 1185313..1186530 (-) 1218 WP_346841809.1 type II secretion system F family protein Machinery gene
  RAL92_RS05590 (RAL92_05595) pilB 1186534..1188237 (-) 1704 WP_346841810.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RAL92_RS05595 (RAL92_05600) - 1188462..1188962 (+) 501 WP_346841811.1 pilin -
  RAL92_RS05600 (RAL92_05605) - 1189075..1190742 (+) 1668 WP_346841812.1 Wzy polymerase domain-containing protein -
  RAL92_RS05610 (RAL92_05615) nadC 1191215..1192063 (-) 849 WP_346841813.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62480.55 Da        Isoelectric Point: 5.6276

>NTDB_id=868212 RAL92_RS05590 WP_346841810.1 1186534..1188237(-) (pilB) [Pseudomonas otitidis strain Tg_9B]
MSESPTLTGLARQLVHANLLDEKAAQSAFIQAQRNKISLVTYLAQNKLLKSRDLAEMAAEQFGIAFMDLASIDKDSQPKD
LVAEKLLRQHRALPLWRRGNKLFIAVSDPTNHQATTDIQFSTGLTTEAILVEDDKLGIALDKFFEGEASGMEDLADVDLD
GLDVEAVEDDKPKSDSGTDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKSYRVRLRTDGILHEVARPPIQLASRISAR
LKVMAALDISERRKPQDGRIKMKISKSKSIDFRVNSLPTLWGEKIVMRILDPSSAQMGIDALGYEESQKELYLSALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPKQGMDFAQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCNNCKKEVEVPRDTLLREGFP
EDKIGTFKIFAPNGCENCKGGYKGRVGIYEVVKNTPSLQRIIMEEGNSIDIAAQMRKDGFNDLRTSGLLKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=868212 RAL92_RS05590 WP_346841810.1 1186534..1188237(-) (pilB) [Pseudomonas otitidis strain Tg_9B]
ATGAGCGAATCGCCCACACTCACAGGCTTGGCTCGGCAACTGGTACACGCCAACTTGCTGGACGAGAAAGCTGCGCAAAG
CGCGTTTATCCAGGCCCAGCGCAACAAAATCTCCCTTGTGACTTACCTTGCTCAAAACAAGCTCCTGAAGAGCCGCGACT
TGGCCGAGATGGCCGCTGAACAGTTCGGCATTGCCTTCATGGACTTGGCAAGCATCGACAAGGACTCGCAACCAAAAGAT
CTGGTTGCAGAGAAGCTCCTACGCCAGCACCGTGCATTGCCGCTCTGGAGACGAGGCAACAAGCTATTCATTGCGGTCTC
TGACCCGACCAACCATCAAGCCACGACCGACATTCAGTTCAGCACCGGCTTGACCACCGAAGCGATTCTCGTCGAGGACG
ACAAGCTCGGTATCGCCCTCGATAAGTTTTTTGAAGGCGAAGCCTCCGGCATGGAGGACCTGGCTGATGTCGACCTCGAT
GGGCTGGATGTTGAGGCCGTTGAAGACGACAAACCGAAGTCCGACAGTGGGACGGATGCTGACGACGCTCCCGTTGTACG
CTTCGTCAACAAGATGCTGTTGGATGCCATCAAGGGCGGCTCATCCGACCTCCATTTCGAGCCTTATGAAAAGAGTTACC
GCGTACGCCTTCGTACTGACGGCATCTTGCATGAGGTAGCTCGGCCCCCTATACAGCTCGCCAGCCGGATCTCGGCCCGC
CTGAAGGTCATGGCAGCACTCGACATCTCCGAACGCCGCAAGCCCCAGGACGGTCGGATAAAGATGAAGATATCCAAGAG
CAAGTCCATCGACTTTCGCGTCAACTCGCTACCTACGTTATGGGGCGAGAAAATCGTCATGCGAATTCTGGACCCCTCGA
GTGCACAGATGGGTATTGATGCGCTGGGATATGAAGAGAGCCAGAAGGAGCTCTACCTGAGTGCGCTGAAGCAGCCCCAG
GGAATGATTCTGGTAACAGGCCCCACCGGTTCGGGCAAGACGGTGTCGCTCTACACAGGCCTGAATATTCTCAATACAGT
CGACATCAATATTTCCACTGCGGAAGATCCGGTGGAGATCAACCTGGAAGGGATCAATCAGGTCAACGTCAACCCCAAGC
AAGGCATGGACTTTGCTCAAGCACTGCGGGCGTTCCTGCGCCAAGACCCTGACGTGATCATGGTGGGTGAGATTCGCGAC
CTGGAAACTGCCGAAATCGCGATCAAAGCAGCCCAGACCGGCCACATGGTGATGTCGACCTTGCACACCAACAGTGCAGC
CGAAACCCTTACTCGATTACGCAACATGGGTGTTCCGGCATTCAACATCGCTACCTCTGTCAACCTGATCATTGCCCAGC
GCCTTGCCCGCAAGCTCTGCAACAACTGCAAGAAAGAGGTTGAGGTCCCCCGTGACACGCTGCTCAGGGAAGGATTCCCC
GAAGACAAGATCGGCACCTTCAAAATTTTCGCCCCGAACGGCTGTGAGAACTGCAAAGGCGGCTACAAGGGCAGGGTCGG
TATTTATGAAGTAGTTAAAAACACGCCCTCCCTGCAACGCATTATCATGGAGGAAGGCAACTCCATCGATATCGCCGCGC
AGATGCGCAAAGATGGTTTCAACGATCTGCGCACCTCGGGGTTGCTGAAGGCGATGCAAGGCGTCACCAGCCTCGAGGAA
GTCAACCGCGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.351

100

0.607
  pilB Acinetobacter baylyi ADP1

58.988

100

0.596
  pilB Legionella pneumophila strain ERS1305867

54.912

100

0.552
  pilB Vibrio cholerae strain A1552

50.088

100

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

50.89

99.118

0.504
  pilB Vibrio campbellii strain DS40M4

50

100

0.503
  pilF Neisseria gonorrhoeae MS11

48.759

99.471

0.485
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.887

93.298

0.372
  pilF Thermus thermophilus HB27

36.945

99.295

0.367