Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   O5O45_RS30310 Genome accession   NZ_CP132203
Coordinates   6813007..6815136 (+) Length   709 a.a.
NCBI ID   WP_305902987.1    Uniprot ID   -
Organism   Hahella sp. HNIBRBA332     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6808007..6820136
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O5O45_RS30290 (O5O45_30290) pilM 6810217..6811281 (+) 1065 WP_216738677.1 pilus assembly protein PilM Machinery gene
  O5O45_RS30295 (O5O45_30295) - 6811281..6811850 (+) 570 WP_305902984.1 PilN domain-containing protein -
  O5O45_RS30300 (O5O45_30300) - 6811854..6812471 (+) 618 WP_305902985.1 type 4a pilus biogenesis protein PilO -
  O5O45_RS30305 (O5O45_30305) - 6812480..6813007 (+) 528 WP_305902986.1 pilus assembly protein PilP -
  O5O45_RS30310 (O5O45_30310) pilQ 6813007..6815136 (+) 2130 WP_305902987.1 type IV pilus secretin PilQ Machinery gene
  O5O45_RS30315 (O5O45_30315) aroK 6815227..6815805 (+) 579 WP_305902988.1 shikimate kinase AroK -
  O5O45_RS30320 (O5O45_30320) aroB 6815817..6816899 (+) 1083 WP_305906259.1 3-dehydroquinate synthase -
  O5O45_RS30325 (O5O45_30325) - 6816914..6818554 (+) 1641 WP_305902989.1 AAA family ATPase -

Sequence

Protein


Download         Length: 709 a.a.        Molecular weight: 76971.37 Da        Isoelectric Point: 4.6686

>NTDB_id=867655 O5O45_RS30310 WP_305902987.1 6813007..6815136(+) (pilQ) [Hahella sp. HNIBRBA332]
MKVYLRPFENLVEKTMTARILKVWLSFIALAISGWAGATSLTGIEFAALPGDRTEIKLQFDSPPPEPRSYSIEQPARIAL
DLEGVANSLAERSHELSKGNTQSVTVLEAGGRTRVIVNLVELVPYTSEVRGDTLVLRLGEGGGNIAPQAQVVNKSSQNAA
STASARSASASATSKPAIAEVDFLRGEKGDGRVLITLTNPKVSVDMSEEAGNIKLVFNGVSLPAELQRRLDVTDFATPVR
IVDSFVESGNATVLIKPEGDYDYLAYQADNQFTLAVEPLTEEESEQRRKDKFPYSGEKLSLNFQDIEVRSVLQLIADFTG
LNLVASDTVGGRITLRLQNVPWDQALELILKTKGLDQRKVGNVLLVAPAEEIAARERLELEASKQVKELAPVRLEVVQIN
YAKAQDIVGLLKEDKDLISSRGFISSDERTNTISIRETAEKLAQVRRLIQTWDVPVSQVLIEARIVRARTDAVSDFGIEW
GGFYDKTNSDSRIVVGGSRGTMVERVNGDPVQFPGALAVDLGVTKTNASSLALGFLKDSRLISMELSALESDGKGEIVAQ
PKIVSADRQTARIESGEEIPYQEATSSGATSISFKKAVLALEVTPQITPDDKIIMDLVVNQDSRGDVTAGIPSIDTNEVK
TQVLVGNGETIVLGGIFTAETSTTTTKTPFLGDVPYLGRLFTKKENTNERSELLIFITPKLITNGLIEN

Nucleotide


Download         Length: 2130 bp        

>NTDB_id=867655 O5O45_RS30310 WP_305902987.1 6813007..6815136(+) (pilQ) [Hahella sp. HNIBRBA332]
ATGAAGGTATACCTTAGGCCATTTGAAAATCTCGTGGAGAAAACAATGACAGCGCGCATCTTGAAAGTATGGTTGTCGTT
TATTGCGCTAGCCATAAGCGGTTGGGCTGGCGCCACCAGTTTAACCGGTATTGAGTTCGCAGCCTTGCCTGGAGACCGGA
CAGAAATAAAACTGCAGTTTGATTCTCCTCCACCAGAGCCTAGGAGCTACAGTATCGAGCAACCGGCTCGAATAGCTTTG
GATCTTGAAGGGGTGGCAAATTCACTAGCTGAGCGTAGCCATGAGCTGTCAAAGGGTAATACTCAAAGCGTTACCGTGTT
AGAAGCTGGTGGTCGAACCAGAGTGATTGTTAATTTAGTTGAACTTGTGCCCTACACTAGCGAAGTTAGAGGCGATACGT
TAGTTCTTCGTCTTGGAGAAGGAGGGGGAAATATTGCCCCACAAGCACAGGTAGTAAACAAGTCTTCACAAAATGCAGCA
TCAACCGCTTCTGCTCGCTCAGCTAGCGCTTCTGCTACCTCAAAGCCTGCAATTGCAGAAGTAGATTTTTTACGAGGTGA
GAAAGGTGATGGCAGGGTTCTAATTACGTTGACGAATCCTAAAGTTAGCGTAGATATGTCAGAAGAGGCTGGTAACATAA
AGCTTGTGTTCAATGGTGTATCGCTTCCGGCTGAGTTGCAGCGTCGGCTAGATGTGACGGACTTTGCTACTCCTGTCCGC
ATCGTCGACTCGTTCGTCGAAAGTGGTAATGCTACTGTATTAATTAAGCCCGAGGGCGATTATGACTACTTGGCTTATCA
GGCCGATAATCAGTTTACTCTGGCTGTAGAGCCTTTGACGGAGGAAGAGTCCGAGCAGCGGCGCAAAGATAAATTTCCTT
ATTCAGGAGAGAAGCTATCTCTCAATTTTCAAGATATTGAAGTTCGTTCAGTGCTGCAGTTGATTGCAGACTTCACCGGA
TTGAACTTAGTTGCAAGTGATACTGTGGGAGGGCGTATTACCCTCAGGCTACAAAATGTTCCTTGGGACCAAGCCTTGGA
ATTAATTCTTAAAACTAAAGGACTAGATCAACGTAAAGTAGGGAATGTTCTTTTGGTTGCCCCGGCTGAAGAAATTGCTG
CGAGGGAAAGACTAGAGCTTGAGGCTAGTAAGCAAGTTAAAGAGTTGGCTCCAGTTAGGCTTGAAGTGGTTCAGATTAAT
TACGCAAAGGCGCAAGATATCGTTGGTCTGCTAAAAGAGGATAAGGATCTTATATCATCTAGAGGCTTTATCTCTTCAGA
TGAAAGGACGAATACCATTAGCATTCGCGAAACCGCAGAAAAGCTGGCTCAGGTGAGAAGGTTAATTCAAACCTGGGATG
TGCCTGTAAGCCAAGTTTTAATTGAGGCTCGAATTGTAAGAGCAAGGACCGATGCTGTTTCTGACTTTGGTATTGAGTGG
GGTGGGTTTTACGACAAAACGAATAGTGACTCTAGAATTGTTGTCGGCGGTTCTCGTGGGACAATGGTAGAGAGAGTCAA
TGGAGACCCGGTTCAATTTCCAGGAGCATTGGCTGTGGATTTGGGGGTTACGAAAACAAATGCAAGTTCTTTGGCCCTAG
GTTTCCTCAAGGATAGTAGGTTAATATCAATGGAGCTATCTGCTCTGGAGTCCGATGGTAAAGGTGAGATCGTCGCACAG
CCTAAGATAGTGAGTGCAGATAGGCAGACTGCTCGAATAGAGTCCGGTGAGGAAATTCCTTATCAAGAAGCAACGTCAAG
TGGCGCGACCTCTATATCATTTAAGAAGGCAGTTTTGGCATTAGAGGTGACCCCTCAGATAACTCCGGATGATAAAATTA
TAATGGATCTGGTTGTTAATCAGGATTCGCGTGGAGATGTAACGGCAGGAATCCCTAGCATCGATACCAACGAAGTGAAG
ACTCAGGTGTTGGTAGGGAATGGTGAGACAATCGTATTGGGTGGAATATTCACAGCAGAGACCTCTACGACGACCACAAA
AACCCCCTTCCTTGGTGATGTTCCTTATTTAGGGAGGCTCTTTACTAAAAAGGAAAATACTAATGAGAGGTCCGAACTCT
TGATCTTCATCACACCAAAACTAATCACTAACGGCCTGATCGAAAACTAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(322-368)

AMIN domain (AMIN)

(52-137)

Bacterial type II and III secretion system protein (Secretin)

(548-702)

Bacterial type II/III secretion system short domain (Secretin_N)

(395-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

49.86

100

0.504
  comQ Acinetobacter baylyi ADP1

39.973

100

0.41
  pilQ Acinetobacter baumannii D1279779

38.219

100

0.394
  pilQ Acinetobacter baumannii strain A118

38.219

100

0.394
  pilQ Legionella pneumophila strain Lp02

39.971

97.038

0.388
  pilQ Legionella pneumophila strain ERS1305867

40.029

96.897

0.388