Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   O5O45_RS30290 Genome accession   NZ_CP132203
Coordinates   6810217..6811281 (+) Length   354 a.a.
NCBI ID   WP_216738677.1    Uniprot ID   -
Organism   Hahella sp. HNIBRBA332     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6805217..6816281
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O5O45_RS30275 (O5O45_30275) rpmE 6805837..6806055 (+) 219 WP_305902981.1 50S ribosomal protein L31 -
  O5O45_RS30280 (O5O45_30280) - 6806296..6807567 (+) 1272 WP_305902982.1 malic enzyme-like NAD(P)-binding protein -
  O5O45_RS30285 (O5O45_30285) - 6807628..6810039 (-) 2412 WP_305902983.1 penicillin-binding protein 1A -
  O5O45_RS30290 (O5O45_30290) pilM 6810217..6811281 (+) 1065 WP_216738677.1 pilus assembly protein PilM Machinery gene
  O5O45_RS30295 (O5O45_30295) - 6811281..6811850 (+) 570 WP_305902984.1 PilN domain-containing protein -
  O5O45_RS30300 (O5O45_30300) - 6811854..6812471 (+) 618 WP_305902985.1 type 4a pilus biogenesis protein PilO -
  O5O45_RS30305 (O5O45_30305) - 6812480..6813007 (+) 528 WP_305902986.1 pilus assembly protein PilP -
  O5O45_RS30310 (O5O45_30310) pilQ 6813007..6815136 (+) 2130 WP_305902987.1 type IV pilus secretin PilQ Machinery gene
  O5O45_RS30315 (O5O45_30315) aroK 6815227..6815805 (+) 579 WP_305902988.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38236.38 Da        Isoelectric Point: 4.2460

>NTDB_id=867654 O5O45_RS30290 WP_216738677.1 6810217..6811281(+) (pilM) [Hahella sp. HNIBRBA332]
MFGLFGKKKQPVLGLDISSTSVKLIELSQQGSRYRVDSYAVEPLPPNAVVEKNINDAEAVGEVISRVVSKARVNTKQAAV
AVAGSAVITKVIQMSSSIREQDLESQIQIEADQYIPYPLDEVALDFEVVGPNEVNAEMMDVLLAACRRENVELREDALEI
GGLTTKVVDVEAYAMERAMGLIEAQLGTGDESQTVAVVDIGATMTTLSVLSDGKTIYTREQLFGGKQLTEEIQRRYGLSQ
EEAGLAKKQGGLPDDYEQEVLHPFKEAVVQQVARSLQFFFSSTQFNEVDYIILAGGTASIPGVAELVQERVGTTTLVANP
FADMTVSSKVNASALSNDAPSLMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=867654 O5O45_RS30290 WP_216738677.1 6810217..6811281(+) (pilM) [Hahella sp. HNIBRBA332]
GTGTTTGGACTATTTGGAAAGAAAAAACAGCCGGTTTTAGGGCTTGATATCAGTTCTACATCGGTCAAGCTGATTGAACT
CAGTCAGCAGGGCAGTCGATATCGAGTGGATAGTTATGCCGTGGAGCCTTTGCCGCCGAACGCAGTCGTTGAAAAGAATA
TCAATGATGCCGAAGCAGTGGGTGAAGTGATCTCAAGAGTTGTAAGCAAGGCTCGGGTCAACACCAAGCAGGCTGCGGTT
GCAGTCGCAGGTTCAGCGGTTATCACCAAAGTCATTCAGATGAGCTCGTCAATTCGAGAGCAAGATCTGGAGTCACAAAT
TCAAATCGAAGCGGACCAGTATATCCCTTATCCTCTGGACGAAGTCGCTCTCGACTTTGAGGTGGTGGGGCCAAACGAAG
TCAACGCAGAGATGATGGACGTTCTCTTGGCTGCGTGCAGACGGGAAAACGTTGAGCTGCGTGAAGATGCTTTAGAGATC
GGCGGCCTTACCACCAAAGTGGTGGATGTCGAGGCTTACGCTATGGAACGAGCCATGGGGCTTATAGAAGCCCAGCTTGG
TACGGGTGATGAGTCTCAAACCGTGGCTGTTGTGGATATCGGCGCCACCATGACCACACTGAGTGTGTTATCAGACGGCA
AGACTATCTACACGCGCGAACAATTATTTGGCGGCAAGCAGCTAACGGAAGAAATACAGCGCCGATACGGTTTGTCGCAA
GAGGAAGCGGGGTTGGCCAAAAAACAAGGCGGCCTACCGGATGACTACGAACAGGAAGTTTTGCATCCTTTCAAAGAGGC
GGTTGTGCAGCAAGTGGCCAGGTCGCTGCAGTTCTTTTTCTCGTCTACTCAGTTTAATGAAGTTGATTACATCATTTTGG
CGGGGGGAACAGCCTCAATTCCTGGTGTCGCTGAACTGGTTCAAGAGCGGGTAGGGACCACGACGCTGGTGGCTAACCCA
TTTGCAGACATGACAGTGTCGTCAAAGGTTAATGCGTCAGCATTAAGTAATGACGCACCCTCCCTCATGATCGCGTGCGG
ATTGGCAATGAGGAGTTTTGACTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.65

100

0.557
  comM Acinetobacter nosocomialis M2

55.65

100

0.557
  comM Acinetobacter baylyi ADP1

53.107

100

0.531
  pilM Legionella pneumophila strain ERS1305867

44.633

100

0.446