Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NGH78_RS09490 Genome accession   NZ_AP024620
Coordinates   1922632..1924293 (-) Length   553 a.a.
NCBI ID   WP_109207340.1    Uniprot ID   -
Organism   Moorella sp. Hama-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1917632..1929293
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGH78_RS09465 (hamaS1_18800) - 1918762..1919514 (-) 753 WP_109207343.1 A24 family peptidase -
  NGH78_RS09470 (hamaS1_18810) - 1919521..1919922 (-) 402 WP_123059989.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NGH78_RS09480 (hamaS1_18820) - 1920199..1921413 (-) 1215 WP_109207342.1 type II secretion system F family protein -
  NGH78_RS09485 (hamaS1_18830) pilT 1921600..1922619 (-) 1020 WP_109207341.1 type IV pilus twitching motility protein PilT Machinery gene
  NGH78_RS09490 (hamaS1_18840) pilF 1922632..1924293 (-) 1662 WP_109207340.1 GspE/PulE family protein Machinery gene
  NGH78_RS09495 (hamaS1_18850) aroB 1924547..1925638 (-) 1092 WP_109207339.1 3-dehydroquinate synthase -
  NGH78_RS09500 (hamaS1_18860) - 1925617..1926138 (-) 522 WP_109207338.1 shikimate kinase -
  NGH78_RS09505 (hamaS1_18870) aroC 1926196..1927362 (-) 1167 WP_109207337.1 chorismate synthase -
  NGH78_RS09510 (hamaS1_18880) - 1927369..1928328 (-) 960 WP_109207336.1 shikimate dehydrogenase -
  NGH78_RS09515 (hamaS1_18890) - 1928364..1928888 (-) 525 WP_235612878.1 YqeG family HAD IIIA-type phosphatase -

Sequence

Protein


Download         Length: 553 a.a.        Molecular weight: 60295.79 Da        Isoelectric Point: 6.1288

>NTDB_id=86725 NGH78_RS09490 WP_109207340.1 1922632..1924293(-) (pilF) [Moorella sp. Hama-1]
MDGRRRLGDLLLEAGMITREQLDQALQEQKRSGERLGKVLLRLGLVTEASMLEVLEFQLGIPKVVLADYHLDPEVVRLVP
EGLARRYQAIPIRLDGNRLLVALADPLNLLALDDLRLVTGKEIMPAIAAEGEIEAALSRFWQQETVTSMSEVAAAVAAAE
SGGRAGGTEGAPAVRLVNSFIQQAIQTRASDIHIEPQEGEVRVRLRVDGLLRELTRLPLGVLSSLISRIKILAGMDIAEK
RLPQDGRFQFTLGKRSVDLRVSSLPTVYGEKVVLRLLDQEAMLLPLDRLGFLPAVQERYNSLVRSAYGMLLITGPTGSGK
TTTLYATLNSLSSPEKNIVTIEDPVEYLLPGINQVRVNPRAGLSFASGLRSILRQDPDIIMVGEIRDRETADIAVRAATT
GHLVFSTLHTNDAAGAITRLLDMGIEAYLVNSSLIGVVAQRLVRQICPRCREAYRPEPGTLERSWLPESEELWRGRGCEG
CHYTGYAGRTAIQEVLVMNEELRGLVAAKAPATALKEAAITAGMVPLIEDGLAKVRQGITTVNEVLRVSLGGL

Nucleotide


Download         Length: 1662 bp        

>NTDB_id=86725 NGH78_RS09490 WP_109207340.1 1922632..1924293(-) (pilF) [Moorella sp. Hama-1]
ATGGACGGTCGCCGGCGCCTGGGGGATCTTTTATTAGAAGCCGGGATGATCACCAGGGAACAGCTGGACCAGGCCCTGCA
GGAGCAGAAACGCAGTGGGGAACGCCTGGGTAAGGTCTTGCTGCGCCTAGGCTTGGTCACCGAAGCCAGCATGCTGGAGG
TCCTGGAATTCCAGCTGGGCATTCCCAAGGTGGTCCTGGCGGACTACCACCTGGACCCGGAGGTAGTCCGCCTGGTGCCG
GAGGGCCTGGCCCGGCGTTACCAGGCTATACCCATCCGCTTGGACGGCAACCGCCTGCTGGTAGCCCTGGCCGACCCCCT
GAACCTCCTGGCCCTGGACGATCTGCGCCTGGTCACCGGCAAGGAGATTATGCCGGCCATCGCCGCCGAGGGGGAGATCG
AGGCAGCTTTAAGCCGGTTTTGGCAGCAGGAAACCGTTACGAGTATGAGCGAAGTGGCGGCAGCCGTCGCTGCCGCGGAA
TCTGGCGGGCGCGCCGGCGGCACTGAAGGCGCGCCGGCTGTGCGCCTGGTCAACAGCTTTATCCAGCAGGCCATCCAGAC
CCGGGCCAGCGACATCCATATTGAGCCCCAGGAGGGGGAAGTCCGGGTGCGCCTGCGGGTGGACGGCCTGCTGCGGGAGT
TGACCCGCCTGCCCCTGGGGGTTCTAAGTAGCCTGATCTCCAGAATCAAGATCCTGGCCGGGATGGATATTGCCGAAAAA
CGGCTGCCCCAGGACGGCCGCTTCCAGTTTACCCTGGGGAAGCGCAGCGTTGACCTGCGGGTTTCCAGCCTGCCGACGGT
CTACGGAGAAAAAGTCGTCCTGCGCCTGCTGGACCAGGAGGCCATGCTCCTGCCCCTGGACCGTCTGGGGTTCCTGCCTG
CCGTTCAGGAACGCTATAACAGCCTGGTCCGGAGTGCTTACGGTATGTTATTGATTACCGGACCAACGGGGAGCGGCAAG
ACGACGACCCTGTACGCCACCCTCAATTCCTTGAGTTCACCGGAGAAAAATATCGTTACCATTGAGGACCCGGTAGAGTA
CCTGCTACCCGGCATCAACCAGGTGCGGGTCAACCCCAGGGCCGGACTCTCCTTTGCCTCCGGCCTGCGTTCCATCCTGC
GCCAGGACCCGGATATCATCATGGTGGGGGAAATCCGGGACCGGGAGACGGCCGATATAGCCGTCCGGGCGGCAACTACC
GGGCACCTGGTCTTCAGCACCCTGCACACCAACGACGCCGCCGGGGCGATAACCCGCCTGCTGGACATGGGTATCGAGGC
CTACCTGGTGAATTCCTCCCTCATCGGCGTGGTGGCCCAGCGATTGGTGCGCCAGATCTGCCCCCGCTGCCGGGAAGCCT
ACCGGCCCGAGCCCGGGACCCTGGAACGATCCTGGCTACCGGAGAGCGAGGAACTCTGGCGCGGCCGGGGCTGTGAGGGC
TGCCACTATACCGGTTATGCCGGTCGGACGGCCATCCAGGAGGTCCTGGTCATGAACGAGGAACTCCGGGGCCTGGTGGC
CGCCAAGGCACCGGCCACGGCCCTGAAGGAGGCGGCCATTACCGCTGGTATGGTACCTTTAATAGAAGATGGGCTGGCCA
AGGTCCGCCAGGGAATCACCACGGTGAACGAGGTCCTGCGGGTTTCCCTGGGGGGCCTGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-144)

Type II/IV secretion system protein (T2SSE)

(180-445)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

48.452

99.277

0.481
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.182

99.458

0.439
  pilB/pilB1 Synechocystis sp. PCC 6803

38.259

100

0.421
  pilB Vibrio cholerae strain A1552

41.516

100

0.416
  pilB Vibrio parahaemolyticus RIMD 2210633

40.747

100

0.414
  pilB Vibrio campbellii strain DS40M4

40.934

100

0.412
  pilB Acinetobacter baylyi ADP1

39.459

100

0.396
  pilB Acinetobacter baumannii D1279779

38.156

100

0.382
  pilB Legionella pneumophila strain ERS1305867

40.351

92.767

0.374
  pilF Neisseria gonorrhoeae MS11

37.226

99.096

0.369

Multiple sequence alignment