Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Q7574_RS16685 Genome accession   NZ_CP131867
Coordinates   3502583..3504295 (+) Length   570 a.a.
NCBI ID   WP_126645060.1    Uniprot ID   -
Organism   Acinetobacter pittii strain 2012N08-034     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3497583..3509295
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q7574_RS16650 (Q7574_16710) nusA 3498471..3499955 (-) 1485 WP_263612804.1 transcription termination factor NusA -
  Q7574_RS16655 (Q7574_16715) rimP 3499993..3500517 (-) 525 WP_151283486.1 ribosome maturation factor RimP -
  Q7574_RS16675 (Q7574_16735) secG 3501155..3501484 (-) 330 WP_016142373.1 preprotein translocase subunit SecG -
  Q7574_RS16680 (Q7574_16740) tpiA 3501497..3502291 (-) 795 WP_069141694.1 triose-phosphate isomerase -
  Q7574_RS16685 (Q7574_16745) pilB 3502583..3504295 (+) 1713 WP_126645060.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Q7574_RS16690 (Q7574_16750) pilC 3504322..3505548 (+) 1227 WP_151283487.1 type II secretion system F family protein Machinery gene
  Q7574_RS16695 (Q7574_16755) pilD 3505548..3506408 (+) 861 WP_108009301.1 A24 family peptidase Machinery gene
  Q7574_RS16700 (Q7574_16760) coaE 3506410..3507006 (+) 597 WP_017402593.1 dephospho-CoA kinase -
  Q7574_RS16705 (Q7574_16765) - 3507003..3507917 (-) 915 WP_151283488.1 DMT family transporter -
  Q7574_RS16710 (Q7574_16770) rlmB 3507953..3508702 (-) 750 WP_016142380.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  Q7574_RS16715 (Q7574_16775) - 3508805..3509131 (-) 327 WP_002115102.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63542.90 Da        Isoelectric Point: 5.0271

>NTDB_id=865643 Q7574_RS16685 WP_126645060.1 3502583..3504295(+) (pilB) [Acinetobacter pittii strain 2012N08-034]
MSVIHTSPKFSGFFRRLVEEKHVSAATMQTALDAAKRAKRDTVAYLIEEVNLSPSLLAETISIEFAEPYFDLDVYDTSQI
PKDLVDQKLVLKHRVLPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKILAQQFTEESNFEFSDEEF
DLDVDLEAPPSQEDEEETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFLEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKTPADIPKQSLLEMGF
TDQDLTHPDFQVFQPVGCTECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=865643 Q7574_RS16685 WP_126645060.1 3502583..3504295(+) (pilB) [Acinetobacter pittii strain 2012N08-034]
ATGTCAGTCATACATACATCTCCAAAATTTTCGGGGTTTTTTCGACGATTAGTCGAAGAAAAACATGTGTCGGCAGCGAC
CATGCAAACAGCATTAGATGCAGCAAAGAGAGCTAAACGGGATACAGTTGCGTACCTGATTGAAGAGGTTAATCTCTCCC
CTTCTTTATTAGCTGAAACAATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGATGTTTACGATACAAGCCAAATT
CCCAAGGATTTGGTCGATCAGAAACTGGTTTTAAAACATCGGGTCTTACCTCTCATACAAAGAGGACAGATTTTATATGT
CGCGACCAGCAACCCGAGTAATATTGAAGCGATTGATGCCATTCGCTTTAATAGCAAGCTCATGGTTGAGCCAGTTATTG
TCGAGCACCATAAGCTTGAGAAAATTCTGGCGCAGCAATTTACCGAAGAAAGTAACTTCGAATTTAGTGATGAAGAGTTT
GATCTTGATGTAGATCTAGAAGCTCCACCCTCACAAGAGGATGAGGAAGAAACACCTCAAGGCGATGAAGCACCTATTGT
TAAATATATTAATAAATTACTCATCGATGCGATTCGTATGGGGGCTTCAGACTTACATTTTGAACCATACGAGAAATCAT
ATCGGGTCCGTTATCGAGTCGACGGCGTGTTACGTCAGATTGCAAATCCACCTTTGCAATTGGCTAACCGTTTGGCTTCA
CGTTTAAAAGTCATGTCTCAAATGGATATTTCCGAAAAACGAGTGCCTCAAGATGGTCGTATCAAGCTTAAGTTATCAAA
GACTAAAGCAATCGATTTTCGTGTGAACTCACTTCCAACTTTGTTTGGTGAAAAACTGGTTCTTCGTATTCTCGATCCTT
CTAGCGCAATGCTCGGAATTGATGCTTTAGGATATGAAGAAGATCAGAAAGCTCTTTTTTTAGAAGCACTCGATAAACCA
CAAGGTATGCTTTTAATTACAGGTCCTACCGGTTCTGGTAAAACGGTATCTTTATATACTGGCTTAAATATTTTAAATAC
TGAAAGCTCTAATATTTCCACTGCCGAAGATCCAGTCGAAATCAACCTTGAAGGCATTAATCAGGTTAACGTTAACCCAA
AAGTAGGTCTTACTTTTTCGGCCGCACTGAAATCATTTTTACGTCAAGACCCTGACATTATTATGGTCGGTGAGATTCGT
GACTTAGAAACGGCTGAAATTGCGATTAAAGCTGCACAAACCGGTCACATGGTAATGTCCACACTGCATACCAATAGTGC
GCCAGAAACACTTACTCGCCTACGTAATATGGGTGTTCCATCCTTTAATATCGCAACGTCTGTCAATCTGGTGATTGCTC
AACGTTTGGCGCGCCGGTTATGCCCACAGTGTAAAACACCTGCCGACATTCCTAAACAGAGCCTATTAGAAATGGGCTTT
ACCGACCAAGATTTAACCCATCCCGATTTTCAAGTTTTTCAACCTGTAGGTTGTACTGAGTGTCGTGAAGGCTACAAAGG
TCGAGTAGGTATTTACGAGGTGATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCTGCTTCTGAAAAACTGGGGTTTAATAATCTGCGTCGTTCAGGGTTAAAGAAAGTCATGCAAGGTGTAACTTCC
TTACAAGAAGTTAACCGCGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

94.561

100

0.946
  pilB Acinetobacter baylyi ADP1

78.07

100

0.781
  pilB Legionella pneumophila strain ERS1305867

51.146

99.474

0.509
  pilF Neisseria gonorrhoeae MS11

49.822

98.596

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

51.527

91.93

0.474
  pilB Vibrio campbellii strain DS40M4

50.766

91.579

0.465
  pilB Vibrio cholerae strain A1552

50.096

91.053

0.456
  pilB/pilB1 Synechocystis sp. PCC 6803

35.016

100

0.377
  pilF Thermus thermophilus HB27

36.655

98.596

0.361