Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K5628_RS02290 Genome accession   NZ_AP024613
Coordinates   480150..481856 (-) Length   568 a.a.
NCBI ID   WP_208146123.1    Uniprot ID   A0AAD1K6N5
Organism   Shewanella algae strain TUM17379     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 475150..486856
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5628_RS02255 - 475405..475674 (-) 270 Protein_423 transposase -
  K5628_RS02260 (TUM17379_04200) mutT 475745..476143 (-) 399 WP_025012058.1 8-oxo-dGTP diphosphatase MutT -
  K5628_RS02265 (TUM17379_04210) yacG 476143..476355 (-) 213 WP_025886789.1 DNA gyrase inhibitor YacG -
  K5628_RS02270 (TUM17379_04220) zapD 476400..477140 (-) 741 WP_028779562.1 cell division protein ZapD -
  K5628_RS02275 (TUM17379_04230) coaE 477137..477748 (-) 612 WP_208146119.1 dephospho-CoA kinase -
  K5628_RS02280 (TUM17379_04240) pilD 477749..478663 (-) 915 WP_208146120.1 A24 family peptidase Machinery gene
  K5628_RS02285 (TUM17379_04250) pilC 478732..479997 (-) 1266 WP_208146121.1 type II secretion system F family protein Machinery gene
  K5628_RS02290 (TUM17379_04260) pilB 480150..481856 (-) 1707 WP_208146123.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K5628_RS02295 (TUM17379_04270) - 481866..482279 (-) 414 WP_025012053.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  K5628_RS02300 (TUM17379_04280) nadC 483147..484007 (-) 861 WP_025012052.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K5628_RS02305 (TUM17379_04290) ampD 484166..484744 (+) 579 WP_208146125.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K5628_RS02310 (TUM17379_04300) ampE 484798..485649 (+) 852 WP_208146127.1 beta-lactamase regulator AmpE -
  K5628_RS02315 (TUM17379_04310) pdhR 485925..486677 (+) 753 WP_025886793.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63091.20 Da        Isoelectric Point: 5.3439

>NTDB_id=86549 K5628_RS02290 WP_208146123.1 480150..481856(-) (pilB) [Shewanella algae strain TUM17379]
MPTSGLHLGLSTLFIRKGLLTENQLTETIRHSQKNKQTLVTTLVASNLLSARTIAELCYEEYGTPLLDLNEFDLNSIPEE
FLNKKLIDKHRCLPLFKRGNRLYIATSDPTNIAALEDFQFSAGLHAEAILVEEDKLSKALEKVLEEDLSSLDLDGLDEES
LAGIEVTDTDKRQQEDGESKDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPISLSGRISAR
LKVMSKLDIAERRVPQDGRIKMRLSRNKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEEDQKQAYLEMLARPQ
GMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSSAETLTRLLNMGVPGYNIASSVNLIIAQRLARRLCPECRQPEDIPEHELLQLGFS
QQQIDAGFTPYKPCGCDLCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIAATAKQQGMRDLRLSGLLKVIQGVTSIA
EINRVTSY

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=86549 K5628_RS02290 WP_208146123.1 480150..481856(-) (pilB) [Shewanella algae strain TUM17379]
ATGCCAACATCCGGCTTACACCTAGGTCTGTCGACTCTTTTTATCCGTAAAGGTCTACTGACGGAAAATCAGTTAACCGA
AACGATAAGACATTCCCAGAAAAATAAACAAACACTGGTCACCACTCTGGTGGCCAGTAATCTTTTATCCGCTCGCACTA
TCGCCGAACTCTGCTATGAAGAGTATGGCACCCCGCTGCTGGATCTCAATGAGTTTGATTTAAACAGTATCCCGGAAGAA
TTTCTCAATAAAAAGCTGATCGATAAGCACCGCTGCCTGCCGTTATTCAAGCGTGGCAACCGCCTCTATATCGCCACTTC
AGACCCCACCAATATTGCCGCCCTGGAAGATTTTCAGTTCAGCGCCGGCTTGCACGCCGAAGCGATACTGGTAGAGGAAG
ATAAGCTCAGTAAAGCACTGGAAAAAGTGCTTGAAGAGGACTTGAGTTCGCTGGATCTCGATGGCCTCGATGAGGAATCC
CTCGCAGGTATCGAGGTCACCGACACCGACAAACGTCAACAGGAAGATGGTGAAAGTAAAGATGACGCCCCCATCGTCAT
CTATATCAATAAGATTTTGACCGATGCCATCCGCAAGGGCGCATCGGATCTGCACTTCGAACCCTACGAGAAGCGCTACC
GCATCCGTTTCCGTATCGATGGCATCCTCCACGAGGTATCTGAGCCCCCCATTTCTCTCTCGGGCCGTATCTCAGCCCGC
TTGAAGGTGATGTCCAAACTGGATATCGCCGAGCGCCGCGTTCCTCAGGATGGCCGCATCAAGATGAGGCTGTCGCGCAA
CAAGAGTATCGATTTCCGGGTCAGTACCCTGCCCACTCTTTGGGGTGAAAAGATAGTGATGCGGATCCTCGACTCGTCTT
CCGCGCAATTAGGCATCGAAAAACTCGGCTATGAGGAAGACCAAAAACAAGCCTACCTGGAGATGCTGGCAAGGCCCCAG
GGGATGATTCTGGTGACAGGCCCTACCGGCTCTGGTAAAACCGTTTCCCTTTATACTGGCCTCAATATCCTCAATACCGA
AGAGCGCAATATCAGCACCGCCGAAGACCCAGTGGAGATCAACCTGGAAGGGGTCAACCAGGTACATATCAACCCCAAGG
CAGGGCTAACCTTCGCCTCGGCGCTGCGCTCCTTTTTGCGTCAGGACCCGGATGTGGTGATGGTGGGGGAAATTCGCGAC
CTGGAAACCGCCGAAATCGCCATTAAGGCGGCACAAACCGGCCACTTGGTACTCTCGACCCTGCATACCAACTCATCGGC
CGAGACTCTCACCCGCCTGCTTAATATGGGGGTTCCCGGTTATAACATTGCCAGTTCGGTCAACCTGATTATTGCCCAGC
GCCTGGCCCGCCGCCTCTGCCCCGAATGCCGCCAACCGGAAGATATTCCCGAGCATGAACTGCTGCAACTGGGCTTCAGT
CAGCAGCAGATAGACGCAGGCTTTACCCCATACAAGCCCTGTGGCTGCGATCTCTGCTCCGGTGGTTATAAGGGCCGGGT
CGGTATCTATGAGGTAATGAAGATGTCCGACGAAATCGCCAGAACCATTATGGAGGGCGGTAATTCGCTGCAGATTGCCG
CCACTGCCAAACAGCAGGGGATGCGTGATTTGCGCCTTTCCGGTCTTTTAAAAGTGATTCAAGGCGTCACCAGTATCGCC
GAGATAAATCGGGTCACCAGCTACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.358

98.768

0.586
  pilB Acinetobacter baumannii D1279779

60.037

94.718

0.569
  pilB Legionella pneumophila strain ERS1305867

55.83

99.648

0.556
  pilB Vibrio parahaemolyticus RIMD 2210633

52.91

99.824

0.528
  pilB Vibrio cholerae strain A1552

53.455

96.831

0.518
  pilB Vibrio campbellii strain DS40M4

51.246

98.944

0.507
  pilF Neisseria gonorrhoeae MS11

47.788

99.472

0.475
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.698

97.359

0.377
  pilF Thermus thermophilus HB27

37.589

99.296

0.373

Multiple sequence alignment