Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K5624_RS02245 Genome accession   NZ_AP024612
Coordinates   476959..478665 (-) Length   568 a.a.
NCBI ID   WP_144224957.1    Uniprot ID   -
Organism   Shewanella algae strain TUM17378     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 471959..483665
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5624_RS02210 - 472269..472469 (-) 201 Protein_407 transposase -
  K5624_RS02215 (TUM17378_04170) mutT 472544..472942 (-) 399 WP_144379648.1 8-oxo-dGTP diphosphatase MutT -
  K5624_RS02220 (TUM17378_04180) yacG 472942..473154 (-) 213 WP_025886789.1 DNA gyrase inhibitor YacG -
  K5624_RS02225 (TUM17378_04190) zapD 473199..473939 (-) 741 WP_028779562.1 cell division protein ZapD -
  K5624_RS02230 (TUM17378_04200) coaE 473936..474547 (-) 612 WP_025012056.1 dephospho-CoA kinase -
  K5624_RS02235 (TUM17378_04210) pilD 474548..475462 (-) 915 WP_144224959.1 A24 family peptidase Machinery gene
  K5624_RS02240 (TUM17378_04220) pilC 475538..476803 (-) 1266 WP_144224958.1 type II secretion system F family protein Machinery gene
  K5624_RS02245 (TUM17378_04230) pilB 476959..478665 (-) 1707 WP_144224957.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K5624_RS02250 (TUM17378_04240) - 478675..479088 (-) 414 WP_144224956.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  K5624_RS02255 (TUM17378_04250) nadC 479946..480806 (-) 861 WP_025012052.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K5624_RS02260 (TUM17378_04260) ampD 480965..481543 (+) 579 WP_144216886.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K5624_RS02265 (TUM17378_04270) ampE 481597..482448 (+) 852 WP_025012050.1 beta-lactamase regulator AmpE -
  K5624_RS02270 (TUM17378_04280) pdhR 482723..483475 (+) 753 WP_025886793.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63092.23 Da        Isoelectric Point: 5.3459

>NTDB_id=86525 K5624_RS02245 WP_144224957.1 476959..478665(-) (pilB) [Shewanella algae strain TUM17378]
MPTSGLHLGLSTLFIRKGLLTENQLTETIRHSQKNKQTLVTTLVASNLLSARTIAELCYEEYGTPLLDLNEFDLSSIPEE
FLNKKLIDKHRCLPLFKRGNRLYIATSDPTNIAALEDFQFSAGLHAEAILVEEDKLSKALEKVLEEDLSSLDLDGLDEES
LAGIEVTDTDKRQQEEGESKDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPISLSGRISAR
LKVMSKLDIAERRVPQDGRIKMRLSRNKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEEDQKQAYLEMLARPQ
GMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSSAETLTRLLNMGVPGYNIASSVNLIIAQRLARRLCPECRQPEDIPEHELLQLGFT
QQQIDAGFTPYKPCGCDLCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIAATAKQQGMRDLRLSGLLKVIQGVTSIA
EINRVTSY

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=86525 K5624_RS02245 WP_144224957.1 476959..478665(-) (pilB) [Shewanella algae strain TUM17378]
ATGCCAACATCCGGCTTACACCTAGGTCTGTCGACTCTTTTTATCCGCAAAGGTCTACTGACGGAAAATCAGTTAACCGA
AACGATAAGACATTCCCAGAAAAATAAACAAACACTGGTCACCACTCTGGTGGCCAGTAATCTTTTATCTGCTCGTACCA
TCGCTGAACTCTGCTATGAAGAATACGGCACCCCACTGCTGGATCTCAATGAGTTTGATTTAAGCAGTATCCCGGAAGAA
TTTCTCAATAAAAAGCTGATCGATAAGCACAGATGCCTGCCGTTATTCAAGCGTGGCAACCGCCTCTATATCGCCACCTC
AGACCCCACCAATATTGCCGCCCTGGAAGACTTCCAGTTCAGCGCCGGCCTACACGCGGAAGCGATACTGGTAGAGGAAG
ATAAGCTCAGTAAAGCACTGGAAAAAGTGCTTGAAGAAGACTTGAGTTCGCTGGATCTCGATGGCCTCGATGAGGAATCC
CTCGCAGGTATCGAGGTCACCGACACCGACAAACGTCAACAGGAAGAGGGTGAAAGTAAAGATGACGCTCCTATCGTCAT
CTATATCAATAAGATTTTGACCGATGCCATCCGTAAGGGCGCATCGGATCTGCATTTCGAGCCTTACGAAAAACGCTACC
GCATTCGCTTTCGTATCGATGGCATCCTCCACGAAGTATCTGAGCCACCAATTTCTCTCTCGGGCCGCATATCAGCCCGC
TTGAAGGTAATGTCCAAACTGGATATCGCCGAGCGCCGGGTCCCTCAGGATGGCCGCATCAAGATGAGGCTGTCGCGCAA
CAAGAGTATCGATTTTCGGGTCAGTACCCTGCCCACCCTCTGGGGTGAAAAGATAGTGATGCGGATCCTAGACTCCTCTT
CCGCGCAATTAGGAATCGAAAAACTCGGCTATGAGGAAGACCAAAAACAAGCCTACCTGGAGATGCTGGCAAGGCCCCAG
GGGATGATCTTGGTCACCGGCCCTACCGGCTCTGGTAAAACCGTATCCCTTTATACTGGCCTCAATATCCTCAATACCGA
AGAGCGTAATATCAGCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGGGTCAACCAGGTGCATATCAACCCCAAGG
CCGGATTAACCTTCGCCTCGGCGCTGCGCTCCTTTTTGCGTCAGGACCCTGATGTGGTGATGGTGGGGGAAATTCGCGAT
CTGGAAACCGCCGAAATCGCCATCAAGGCGGCACAAACCGGCCACTTGGTACTCTCGACCCTGCATACCAACTCATCGGC
CGAAACCCTTACCCGCCTGCTCAATATGGGTGTCCCTGGTTATAACATTGCCAGTTCTGTCAATCTGATTATTGCCCAGC
GCCTGGCCCGCCGCCTCTGCCCCGAATGCCGTCAACCGGAAGATATCCCCGAGCATGAACTGCTGCAACTGGGCTTCACT
CAGCAGCAGATAGACGCAGGCTTTACCCCTTACAAGCCCTGTGGCTGCGATCTCTGCTCCGGTGGCTATAAGGGCCGGGT
CGGTATCTATGAGGTAATGAAGATGTCCGATGAAATCGCCAGAACCATTATGGAGGGTGGTAACTCACTGCAGATTGCCG
CCACCGCCAAGCAGCAGGGAATGAGGGACTTGCGCCTTTCCGGCCTTTTAAAAGTGATTCAAGGCGTCACCAGTATCGCC
GAGATAAATCGGGTCACCAGCTACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.358

98.768

0.586
  pilB Acinetobacter baumannii D1279779

60.595

94.718

0.574
  pilB Legionella pneumophila strain ERS1305867

55.654

99.648

0.555
  pilB Vibrio parahaemolyticus RIMD 2210633

53.086

99.824

0.53
  pilB Vibrio cholerae strain A1552

53.636

96.831

0.519
  pilB Vibrio campbellii strain DS40M4

51.51

99.12

0.511
  pilF Neisseria gonorrhoeae MS11

48.496

99.472

0.482
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.71

98.239

0.38
  pilF Thermus thermophilus HB27

37.589

99.296

0.373

Multiple sequence alignment