Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K5626_RS02220 Genome accession   NZ_AP024610
Coordinates   473023..474729 (-) Length   568 a.a.
NCBI ID   WP_144159907.1    Uniprot ID   -
Organism   Shewanella algae strain TUM4442     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 468023..479729
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5626_RS02185 - 468264..468533 (-) 270 Protein_403 transposase -
  K5626_RS02190 (TUM4442_04140) mutT 468608..469006 (-) 399 WP_025012058.1 8-oxo-dGTP diphosphatase MutT -
  K5626_RS02195 (TUM4442_04150) yacG 469006..469218 (-) 213 WP_025886789.1 DNA gyrase inhibitor YacG -
  K5626_RS02200 (TUM4442_04160) zapD 469263..470003 (-) 741 WP_028779562.1 cell division protein ZapD -
  K5626_RS02205 (TUM4442_04170) coaE 470000..470611 (-) 612 WP_054744929.1 dephospho-CoA kinase -
  K5626_RS02210 (TUM4442_04180) pilD 470612..471526 (-) 915 WP_144220359.1 A24 family peptidase Machinery gene
  K5626_RS02215 (TUM4442_04190) pilC 471596..472864 (-) 1269 WP_144220360.1 type II secretion system F family protein Machinery gene
  K5626_RS02220 (TUM4442_04200) pilB 473023..474729 (-) 1707 WP_144159907.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K5626_RS02225 (TUM4442_04210) - 474775..475206 (-) 432 WP_144220361.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  K5626_RS02230 (TUM4442_04220) nadC 476075..476935 (-) 861 WP_025012052.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K5626_RS02235 (TUM4442_04230) ampD 477127..477672 (+) 546 WP_025012051.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K5626_RS02240 (TUM4442_04240) ampE 477726..478577 (+) 852 WP_025012050.1 beta-lactamase regulator AmpE -
  K5626_RS02245 (TUM4442_04250) pdhR 478852..479604 (+) 753 WP_025886793.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63005.10 Da        Isoelectric Point: 5.2529

>NTDB_id=86501 K5626_RS02220 WP_144159907.1 473023..474729(-) (pilB) [Shewanella algae strain TUM4442]
MPTSGLHLGLSTLFIRKGLLNEGSLSDAIKQSQKNKQTLVTTLVTSQLLSARTIAELCYEEYGTPLLDLNEFDLSSIPEE
FLNKKLIDKHRCLPLFKRGNRLYIATSDPTNIAALEDFQFSAGLHAEAILVEEDKLSKALEKVLEEDLSSLDLDGLDEES
LSGIEVTDADKRQQEENEGKDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPISLSGRISAR
LKVMSKLDIAERRVPQDGRIKMKLSRNKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEEDQKQAYLEMLARPQ
GMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSSAETLTRLLNMGVPGYNIASSVNLIIAQRLARRLCPECRQPEDIPEHELLQLGFS
QQQIDAGFTPYKPCGCEFCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIATTAKQQGMRDLRLSGLLKVIQGVTSIA
EINRVTSY

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=86501 K5626_RS02220 WP_144159907.1 473023..474729(-) (pilB) [Shewanella algae strain TUM4442]
ATGCCTACTTCTGGGTTACATTTAGGATTGTCCACTCTGTTTATCCGTAAAGGGCTACTCAATGAGGGTAGCCTTTCGGA
TGCCATTAAGCAGTCGCAAAAGAACAAACAAACACTGGTCACCACTCTGGTGACCAGTCAACTTTTATCCGCCCGCACTA
TCGCTGAGCTCTGTTATGAAGAGTATGGCACCCCGCTGCTGGATCTCAATGAGTTTGATTTAAGCAGTATCCCGGAAGAG
TTTCTCAATAAAAAGCTAATCGACAAGCACCGCTGCCTGCCGTTATTCAAGCGTGGCAACCGTCTCTATATCGCCACATC
AGACCCCACCAATATTGCCGCTCTGGAAGATTTTCAGTTCAGCGCCGGCCTCCATGCCGAGGCGATACTGGTAGAGGAAG
ATAAACTCAGTAAAGCACTAGAAAAAGTGCTTGAAGAGGACTTGAGTTCATTAGATCTGGATGGCCTCGACGAGGAATCC
CTCTCCGGTATCGAAGTCACGGATGCTGACAAGCGTCAGCAGGAAGAGAATGAAGGCAAAGACGATGCTCCTATCGTCAT
CTATATCAATAAGATTTTGACCGATGCCATCCGCAAGGGCGCATCGGATCTGCATTTCGAGCCCTACGAGAAACGCTACC
GCATCCGTTTTCGTATCGATGGTATTCTCCACGAGGTATCTGAGCCACCCATTTCTCTCTCGGGCCGTATATCAGCCCGC
TTGAAAGTGATGTCCAAACTGGATATCGCCGAGCGCCGGGTACCTCAGGATGGCCGCATCAAGATGAAGCTGTCCCGCAA
CAAGAGTATCGATTTCCGTGTCAGCACCCTGCCCACCCTCTGGGGTGAAAAGATAGTAATGCGGATCCTCGACTCCTCTT
CCGCGCAATTAGGCATCGAAAAACTCGGCTATGAGGAAGATCAAAAACAAGCCTACCTGGAGATGCTGGCAAGACCCCAG
GGGATGATCTTGGTCACCGGCCCTACCGGCTCGGGTAAAACCGTATCCCTTTATACTGGCCTCAATATCCTCAATACCGA
AGAGCGCAATATCAGCACTGCCGAAGACCCGGTGGAGATCAACCTGGAAGGGGTCAACCAGGTACATATCAACCCCAAGG
CCGGGCTAACCTTCGCCTCGGCGCTGCGCTCTTTTTTACGTCAAGACCCTGATGTGGTGATGGTGGGGGAAATTCGCGAC
CTGGAAACCGCCGAAATCGCCATCAAGGCGGCGCAAACTGGCCACTTGGTACTCTCAACCCTGCATACCAACTCATCGGC
CGAGACTCTCACCCGCCTGCTCAACATGGGTGTCCCAGGTTATAACATTGCCAGTTCAGTCAATCTGATTATTGCCCAGC
GTCTGGCCCGCCGCCTTTGCCCCGAATGCCGCCAACCGGAAGATATCCCCGAGCATGAACTGCTGCAACTGGGCTTCAGT
CAGCAGCAGATAGACGCAGGATTTACCCCATATAAGCCCTGCGGCTGCGAATTCTGCTCAGGTGGCTACAAGGGCCGGGT
CGGTATCTATGAGGTGATGAAGATGTCTGATGAAATCGCCAGAACCATTATGGAGGGCGGTAATTCGCTGCAGATTGCCA
CCACCGCCAAACAACAGGGGATGCGCGACCTGCGCCTTTCCGGCCTTTTAAAGGTGATTCAAGGCGTCACCAGTATCGCC
GAGATCAATCGGGTCACCAGCTACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.358

98.768

0.586
  pilB Acinetobacter baumannii D1279779

58.099

100

0.581
  pilB Legionella pneumophila strain ERS1305867

56.483

99.12

0.56
  pilB Vibrio parahaemolyticus RIMD 2210633

52.91

99.824

0.528
  pilB Vibrio campbellii strain DS40M4

51.068

98.944

0.505
  pilB Vibrio cholerae strain A1552

54.685

92.077

0.504
  pilF Neisseria gonorrhoeae MS11

47.257

99.472

0.47
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.42

98.063

0.377
  pilF Thermus thermophilus HB27

37.655

99.12

0.373

Multiple sequence alignment