Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   Q7613_RS03810 Genome accession   NZ_CP131720
Coordinates   747577..748026 (+) Length   149 a.a.
NCBI ID   WP_011271973.1    Uniprot ID   Q4QNP4
Organism   Haemophilus influenzae strain 2016N07-308     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 742577..753026
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q7613_RS03790 (Q7613_03790) rsmE 743042..743779 (-) 738 WP_011271976.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  Q7613_RS03795 (Q7613_03795) lnt 743829..745358 (-) 1530 WP_079989936.1 apolipoprotein N-acyltransferase -
  Q7613_RS03800 (Q7613_03800) corC 745381..746280 (-) 900 WP_005656283.1 CNNM family magnesium/cobalt transport protein CorC -
  Q7613_RS03805 (Q7613_03805) ampD 746902..747462 (-) 561 WP_070828280.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  Q7613_RS03810 (Q7613_03810) pilA 747577..748026 (+) 450 WP_011271973.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  Q7613_RS03815 (Q7613_03815) pilB 748023..749417 (+) 1395 WP_011271972.1 GspE/PulE family protein Machinery gene
  Q7613_RS03820 (Q7613_03820) pilC 749414..750634 (+) 1221 WP_011271971.1 type II secretion system F family protein Machinery gene
  Q7613_RS03825 (Q7613_03825) pilD 750631..751323 (+) 693 WP_038440789.1 A24 family peptidase Machinery gene
  Q7613_RS03830 (Q7613_03830) rho 751377..752639 (-) 1263 WP_005629550.1 transcription termination factor Rho -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15572.89 Da        Isoelectric Point: 8.0922

>NTDB_id=864724 Q7613_RS03810 WP_011271973.1 747577..748026(+) (pilA) [Haemophilus influenzae strain 2016N07-308]
MKLTTQQTLKKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAVSELLQASAPYKADVELCVYSTNETTNCTGGKNGIA
ADITTAKGYVKSVTTSNGAITVKGDGTLANMEYILQATGNAATGVTWTTTCKGTDASLFPANFCGSVTQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=864724 Q7613_RS03810 WP_011271973.1 747577..748026(+) (pilA) [Haemophilus influenzae strain 2016N07-308]
ATGAAACTAACAACACAGCAAACCTTGAAAAAAGGGTTTACATTAATAGAGCTAATGATTGTGATTGCAATTATTGCTAT
TTTAGCCACTATCGCAATTCCCTCTTATCAAAATTATACTAAAAAAGCAGCGGTATCTGAATTACTGCAAGCGTCAGCGC
CTTATAAGGCTGATGTGGAATTATGTGTATATAGCACAAATGAAACAACAAACTGTACGGGTGGAAAAAATGGTATTGCA
GCAGATATAACCACAGCAAAAGGCTATGTAAAATCAGTGACAACAAGCAACGGTGCAATAACAGTAAAAGGGGATGGCAC
ATTGGCAAATATGGAATATATTTTGCAAGCTACAGGTAATGCTGCAACAGGTGTAACTTGGACAACAACTTGCAAAGGAA
CGGATGCCTCTTTATTTCCAGCAAATTTTTGCGGAAGTGTCACACAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q4QNP4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

100

100

1
  pilA Haemophilus influenzae Rd KW20

91.946

100

0.919
  pilA Glaesserella parasuis strain SC1401

54.362

100

0.544
  pilA2 Legionella pneumophila str. Paris

41.497

98.658

0.409
  pilA2 Legionella pneumophila strain ERS1305867

41.497

98.658

0.409
  pilA Vibrio campbellii strain DS40M4

39.716

94.631

0.376