Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   Q7615_RS04465 Genome accession   NZ_CP131718
Coordinates   833740..834189 (+) Length   149 a.a.
NCBI ID   WP_011271973.1    Uniprot ID   Q4QNP4
Organism   Haemophilus influenzae strain 2016S11-301     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 828740..839189
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q7615_RS04445 (Q7615_04445) rsmE 829205..829942 (-) 738 WP_038440782.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  Q7615_RS04450 (Q7615_04450) lnt 829995..831521 (-) 1527 WP_080291958.1 apolipoprotein N-acyltransferase -
  Q7615_RS04455 (Q7615_04455) corC 831544..832443 (-) 900 WP_005656283.1 CNNM family magnesium/cobalt transport protein CorC -
  Q7615_RS04460 (Q7615_04460) ampD 833065..833625 (-) 561 WP_011271974.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  Q7615_RS04465 (Q7615_04465) pilA 833740..834189 (+) 450 WP_011271973.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  Q7615_RS04470 (Q7615_04470) pilB 834186..835580 (+) 1395 WP_011271972.1 GspE/PulE family protein Machinery gene
  Q7615_RS04475 (Q7615_04475) pilC 835577..836797 (+) 1221 WP_011271971.1 type II secretion system F family protein Machinery gene
  Q7615_RS04480 (Q7615_04480) pilD 836794..837486 (+) 693 WP_105886719.1 A24 family peptidase Machinery gene
  Q7615_RS04485 (Q7615_04485) rho 837540..838802 (-) 1263 WP_015701557.1 transcription termination factor Rho -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15572.89 Da        Isoelectric Point: 8.0922

>NTDB_id=864651 Q7615_RS04465 WP_011271973.1 833740..834189(+) (pilA) [Haemophilus influenzae strain 2016S11-301]
MKLTTQQTLKKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAVSELLQASAPYKADVELCVYSTNETTNCTGGKNGIA
ADITTAKGYVKSVTTSNGAITVKGDGTLANMEYILQATGNAATGVTWTTTCKGTDASLFPANFCGSVTQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=864651 Q7615_RS04465 WP_011271973.1 833740..834189(+) (pilA) [Haemophilus influenzae strain 2016S11-301]
ATGAAACTAACAACACAGCAAACCTTGAAAAAAGGGTTTACATTAATAGAGCTAATGATTGTGATTGCAATTATTGCTAT
TTTAGCCACTATCGCAATTCCCTCTTATCAAAATTATACTAAAAAAGCAGCGGTATCTGAATTACTGCAAGCGTCAGCGC
CTTATAAGGCTGATGTGGAATTATGTGTATATAGCACAAATGAAACAACAAACTGTACGGGTGGAAAAAATGGTATTGCA
GCAGATATAACCACAGCAAAAGGCTATGTAAAATCAGTGACAACAAGCAACGGTGCAATAACAGTAAAAGGGGATGGCAC
ATTGGCAAATATGGAATATATTTTGCAAGCTACAGGTAATGCTGCAACAGGTGTAACTTGGACAACAACTTGCAAAGGAA
CGGATGCCTCTTTATTTCCAGCAAATTTTTGCGGAAGTGTCACACAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q4QNP4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

100

100

1
  pilA Haemophilus influenzae Rd KW20

91.946

100

0.919
  pilA Glaesserella parasuis strain SC1401

54.362

100

0.544
  pilA2 Legionella pneumophila str. Paris

41.497

98.658

0.409
  pilA2 Legionella pneumophila strain ERS1305867

41.497

98.658

0.409
  pilA Vibrio campbellii strain DS40M4

39.716

94.631

0.376