Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Q7615_RS04470 Genome accession   NZ_CP131718
Coordinates   834186..835580 (+) Length   464 a.a.
NCBI ID   WP_011271972.1    Uniprot ID   -
Organism   Haemophilus influenzae strain 2016S11-301     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 829186..840580
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q7615_RS04445 (Q7615_04445) rsmE 829205..829942 (-) 738 WP_038440782.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  Q7615_RS04450 (Q7615_04450) lnt 829995..831521 (-) 1527 WP_080291958.1 apolipoprotein N-acyltransferase -
  Q7615_RS04455 (Q7615_04455) corC 831544..832443 (-) 900 WP_005656283.1 CNNM family magnesium/cobalt transport protein CorC -
  Q7615_RS04460 (Q7615_04460) ampD 833065..833625 (-) 561 WP_011271974.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  Q7615_RS04465 (Q7615_04465) pilA 833740..834189 (+) 450 WP_011271973.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  Q7615_RS04470 (Q7615_04470) pilB 834186..835580 (+) 1395 WP_011271972.1 GspE/PulE family protein Machinery gene
  Q7615_RS04475 (Q7615_04475) pilC 835577..836797 (+) 1221 WP_011271971.1 type II secretion system F family protein Machinery gene
  Q7615_RS04480 (Q7615_04480) pilD 836794..837486 (+) 693 WP_105886719.1 A24 family peptidase Machinery gene
  Q7615_RS04485 (Q7615_04485) rho 837540..838802 (-) 1263 WP_015701557.1 transcription termination factor Rho -
  Q7615_RS04490 (Q7615_04490) metJ 839050..839367 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  Q7615_RS04495 (Q7615_04495) - 839381..839767 (-) 387 WP_105886720.1 Cu(I)-responsive transcriptional regulator -
  Q7615_RS04500 (Q7615_04500) - 839844..840050 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  Q7615_RS04505 (Q7615_04505) - 840136..840342 (+) 207 WP_005656255.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52962.30 Da        Isoelectric Point: 5.6248

>NTDB_id=864652 Q7615_RS04470 WP_011271972.1 834186..835580(+) (pilB) [Haemophilus influenzae strain 2016S11-301]
MTSYALLHTQRVTAQNGEIFTISPDLWERNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPHQMQVEEQVKFYQHQETHFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFAELG
MTENQQQAFQRSLSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLLR
AFLRQDPDIIMLGEIRDEESARIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQHEIENSLLLVIAQRLVRKICPKC
GGNLINSCDCHQGYRGRIGVYQFLHWQQNGYQTDFENLRESGLEKVSQGITDEKEIERVLGKNS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=864652 Q7615_RS04470 WP_011271972.1 834186..835580(+) (pilB) [Haemophilus influenzae strain 2016S11-301]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAACCGCTCAAAATGGCGAGATCTTTACGATCTCGCCAGATTTATG
GGAACGCAATCAGCAGCAACAATCCTTGCTCTTGCGGTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCTTGTGAAACCATTGCGTTTATAACAGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGCACCAAATGCAAGTGGAAGAGCAAGTTAAATTCTA
TCAACATCAAGAAACCCATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGACGATTTCAATTTAAAACCACTTTTTCCGATATTCTTGATTTTCGCCTTTCAACCT
TACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCTGTAGAACTTAGCTTTGCTGAACTGGGT
ATGACCGAAAATCAGCAACAAGCATTTCAACGCTCACTTAGCCAGCCACAAGGATTAATTTTAGTAACCGGCCCCACAGG
AAGTGGGAAAAGTATCTCGCTTTACACCGCACTTCAGTGGCTAAATACGCCTGATAAACATATTATGACCGCTGAAGATC
CCATTGAAATTGAACTTGATGGTATTATTCAAAGCCAAATTAATCCGCAGATTGGATTAGATTTTAGCCGTCTATTGCGT
GCTTTTTTACGTCAAGATCCCGACATCATTATGCTAGGTGAAATTCGAGATGAAGAAAGTGCAAGGATTGCACTACGTGC
CGCTCAAACGGGACATTTGGTGCTTTCAACTTTACATACCAATGATGCAATATCTGCCATTTCTCGCTTACAACAACTCG
GTATTCAACAACATGAAATTGAAAACAGTTTACTACTCGTCATTGCACAGCGTCTTGTACGAAAAATCTGTCCAAAGTGC
GGTGGAAATTTAATAAATTCTTGTGATTGCCATCAAGGTTATCGAGGGCGAATCGGCGTGTATCAATTTCTACATTGGCA
ACAGAATGGCTATCAAACGGATTTTGAGAATTTACGAGAGAGTGGTTTGGAAAAAGTTAGCCAAGGCATAACAGATGAGA
AAGAAATTGAACGTGTGTTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-98)

Type II/IV secretion system protein (T2SSE)

(128-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

100

100

1
  pilB Haemophilus influenzae Rd KW20

96.544

99.784

0.963
  pilB Glaesserella parasuis strain SC1401

57.456

98.276

0.565
  pilB Vibrio cholerae strain A1552

39.658

100

0.45
  pilB Vibrio campbellii strain DS40M4

40.082

100

0.422
  pilB Vibrio parahaemolyticus RIMD 2210633

39.959

100

0.416
  pilB Legionella pneumophila strain ERS1305867

39.468

100

0.416
  pilB Acinetobacter baylyi ADP1

38.105

100

0.407
  pilB Acinetobacter baumannii D1279779

38.669

100

0.401
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.393

100

0.377
  pilF Neisseria gonorrhoeae MS11

36.555

100

0.375
  pilF Thermus thermophilus HB27

36.134

100

0.371