Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Q5709_RS01235 Genome accession   NZ_CP131483
Coordinates   266276..267985 (-) Length   569 a.a.
NCBI ID   WP_005016986.1    Uniprot ID   -
Organism   Acinetobacter radioresistens strain MC-14     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 261276..272985
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q5709_RS01205 (Q5709_01205) - 261408..261731 (+) 324 WP_005016998.1 pyrimidine/purine nucleoside phosphorylase -
  Q5709_RS01210 (Q5709_01210) rlmB 261835..262590 (+) 756 WP_005016996.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  Q5709_RS01215 (Q5709_01215) - 262615..263550 (+) 936 WP_005016993.1 DMT family transporter -
  Q5709_RS01220 (Q5709_01220) coaE 263547..264158 (-) 612 WP_005016991.1 dephospho-CoA kinase -
  Q5709_RS01225 (Q5709_01225) pilD 264160..265020 (-) 861 WP_005016989.1 prepilin peptidase Machinery gene
  Q5709_RS01230 (Q5709_01230) pilC 265020..266246 (-) 1227 WP_005016988.1 type II secretion system F family protein Machinery gene
  Q5709_RS01235 (Q5709_01235) pilB 266276..267985 (-) 1710 WP_005016986.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Q5709_RS01240 (Q5709_01240) tpiA 268274..269068 (+) 795 WP_005016984.1 triose-phosphate isomerase -
  Q5709_RS01245 (Q5709_01245) secG 269079..269408 (+) 330 WP_005016983.1 preprotein translocase subunit SecG -
  Q5709_RS01265 (Q5709_01265) rimP 270035..270559 (+) 525 WP_005016981.1 ribosome maturation factor RimP -
  Q5709_RS01270 (Q5709_01270) nusA 270600..272084 (+) 1485 WP_005016980.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63640.95 Da        Isoelectric Point: 5.0245

>NTDB_id=862627 Q5709_RS01235 WP_005016986.1 266276..267985(-) (pilB) [Acinetobacter radioresistens strain MC-14]
MTVNFTPKFSGFIRRLVEDNHVSAAEMQNAVDTAKRNRQDIVPYLIDHFKLSPLLIAEFISIEFGEALFDLAVYDTVQIP
RDVVDEKLILKHRVLPLIKRGHTLFVATSNPTNIEALEAVRFNSKLNIEVVIVEHDKLQRTIEQNFSEESNFDFGDEELN
IDVSTSNLDEDDSQDNTPQEEEAPIVKYINKLLVDAIRIGASDLHFEPYEKYYRVRYRVDGVLRQVATPPLQLATRLASR
LKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPEQKALFIEALEKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNNKVGLSFSAALKSFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVAAFNIATSVNLVIAQRLARRLCNQCKVPAEIPRNSLLEMGFT
EDDLAIPDFQVYQPVGCPECRDGYRGRVGIYEVMKVTPEISKIIMEDGNALEIAATAARLGFDNLRRSGLKKVMQGVTSL
QEINRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=862627 Q5709_RS01235 WP_005016986.1 266276..267985(-) (pilB) [Acinetobacter radioresistens strain MC-14]
ATGACAGTTAATTTTACTCCAAAGTTTTCCGGTTTTATTCGCCGTCTGGTTGAGGATAACCACGTGTCTGCTGCTGAAAT
GCAGAATGCGGTCGATACTGCTAAAAGAAACCGGCAGGATATCGTGCCTTATCTGATCGATCATTTTAAACTTTCGCCCT
TACTGATTGCAGAATTTATCTCGATTGAGTTTGGTGAAGCTTTATTCGATTTGGCTGTTTACGATACTGTCCAGATTCCA
CGAGATGTAGTTGATGAGAAACTGATACTCAAGCATCGGGTCTTGCCACTTATTAAGCGTGGTCATACCCTGTTTGTTGC
TACCAGTAATCCCACCAATATTGAAGCTTTAGAAGCTGTCCGGTTTAACAGTAAACTGAATATTGAAGTTGTTATTGTCG
AGCATGACAAACTGCAGCGTACTATTGAGCAGAATTTTTCTGAAGAAAGCAATTTTGATTTTGGTGATGAAGAACTCAAT
ATTGATGTTTCAACCAGCAATTTAGATGAGGACGACAGTCAAGATAATACGCCTCAGGAAGAAGAAGCTCCAATTGTTAA
ATATATTAACAAGTTATTGGTAGATGCCATTCGTATTGGTGCATCAGACTTACATTTTGAGCCTTATGAAAAATATTACC
GGGTACGTTACCGGGTCGATGGCGTGTTAAGACAGGTTGCAACACCACCTTTACAGCTGGCTACCCGACTGGCTTCACGT
TTAAAGGTTATGTCACAGATGGATATTTCCGAAAAGCGCGTTCCCCAAGATGGCCGGATCAAATTAAAACTCTCTAAAAC
CAAAGCTATTGATTTCCGTGTGAACTCACTGCCTACACTATTTGGAGAAAAACTGGTTCTGCGTATTTTGGATCCGTCCA
GCGCCATGCTGGGTATTGATGCATTAGGCTATGAACCCGAGCAAAAAGCCCTGTTTATAGAAGCTTTAGAAAAGCCGCAA
GGTATGTTACTCATTACCGGGCCTACCGGTTCCGGTAAAACCGTATCGCTTTATACAGGCCTCAATATTTTAAACCGTGA
GGATACCAATATTTCGACTGCCGAAGACCCGGTAGAAATTAATCTTGAAGGTATTAACCAGGTCAATGTGAACAATAAAG
TCGGGTTAAGCTTCTCTGCCGCGCTAAAGTCATTCCTGCGTCAGGATCCAGATATTATTATGGTAGGTGAGATTCGTGAC
CTGGAAACAGCAGAGATTGCCATTAAAGCAGCTCAGACCGGTCACATGGTTATGTCTACCCTGCATACCAACAGTGCTCC
TGAAACACTAACACGCCTAAGAAATATGGGCGTAGCAGCATTTAATATTGCCACTTCAGTTAATCTGGTCATTGCACAGC
GTCTAGCTCGACGTTTATGCAACCAGTGCAAGGTACCAGCAGAGATTCCTAGAAATAGTCTGCTTGAGATGGGCTTTACC
GAAGATGATCTAGCCATCCCTGATTTTCAGGTTTATCAACCTGTCGGTTGCCCGGAATGTCGTGATGGTTATCGGGGTCG
TGTGGGTATCTATGAAGTAATGAAAGTTACACCGGAAATTTCAAAAATTATTATGGAAGACGGTAACGCCCTTGAAATAG
CAGCAACAGCAGCACGCCTGGGTTTTGATAACTTGCGTCGTTCTGGCCTGAAGAAAGTCATGCAAGGTGTAACCTCTTTA
CAAGAAATTAACCGTGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(56-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.028

99.121

0.803
  pilB Acinetobacter baylyi ADP1

78.153

98.946

0.773
  pilB Legionella pneumophila strain ERS1305867

53.309

95.606

0.51
  pilF Neisseria gonorrhoeae MS11

49.644

98.77

0.49
  pilB Vibrio parahaemolyticus RIMD 2210633

51.248

91.564

0.469
  pilB Vibrio cholerae strain A1552

50.478

91.916

0.464
  pilB Vibrio campbellii strain DS40M4

49.904

91.916

0.459
  pilB/pilB1 Synechocystis sp. PCC 6803

34.355

100

0.374
  pilF Thermus thermophilus HB27

36.879

99.121

0.366