NTDB

Overview

Name	pilD	Type	Machinery gene
Locus tag	Q5709_RS01225	Genome accession	NZ_CP131483
Coordinates	264160..265020 (-)	Length	286 a.a.
NCBI ID	WP_005016989.1	Uniprot ID	A0A062C1I7
Organism	Acinetobacter radioresistens strain MC-14
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 259160..270020

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Q5709_RS01200 (Q5709_01200)	-	261217..261354 (+)	138	WP_005405135.1	hypothetical protein	-
Q5709_RS01205 (Q5709_01205)	-	261408..261731 (+)	324	WP_005016998.1	pyrimidine/purine nucleoside phosphorylase	-
Q5709_RS01210 (Q5709_01210)	rlmB	261835..262590 (+)	756	WP_005016996.1	23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB	-
Q5709_RS01215 (Q5709_01215)	-	262615..263550 (+)	936	WP_005016993.1	DMT family transporter	-
Q5709_RS01220 (Q5709_01220)	coaE	263547..264158 (-)	612	WP_005016991.1	dephospho-CoA kinase	-
Q5709_RS01225 (Q5709_01225)	pilD	264160..265020 (-)	861	WP_005016989.1	prepilin peptidase	Machinery gene
Q5709_RS01230 (Q5709_01230)	pilC	265020..266246 (-)	1227	WP_005016988.1	type II secretion system F family protein	Machinery gene
Q5709_RS01235 (Q5709_01235)	pilB	266276..267985 (-)	1710	WP_005016986.1	type IV-A pilus assembly ATPase PilB	Machinery gene
Q5709_RS01240 (Q5709_01240)	tpiA	268274..269068 (+)	795	WP_005016984.1	triose-phosphate isomerase	-
Q5709_RS01245 (Q5709_01245)	secG	269079..269408 (+)	330	WP_005016983.1	preprotein translocase subunit SecG	-

Sequence

Protein

Download Length: 286 a.a. Molecular weight: 31915.24 Da Isoelectric Point: 7.4686

                                >NTDB_id=862625 Q5709_RS01225 WP_005016989.1 264160..265020(-) (pilD) [Acinetobacter radioresistens strain MC-14]

MQELITLLSNNPTALYIVVGLFSLCIGSFLNVVILRTPKMMEQEWRNECQLLLHPDQPLIDETKLTLSYPASTCPNCHTP

IRWYQNLPLVSWLLLRGKCGQCQNPISIRYPLVELLTALCSLIVVAVYGPTLQMLFGLLLTWALITLTFIDFDTQLLPDR

YTLPLAALGLALNSYALYTSASSAIWGYVIGFLCLWIVYYLFKVITGKEGMGYGDFKLLAALGAWMGPLMLPLIVLLSSL

VGAIIGIVLLKIRKENQPFAFGPYIAIAGWIAFLWGEQIMKIYLGG

Nucleotide

Download Length: 861 bp

                                >NTDB_id=862625 Q5709_RS01225 WP_005016989.1 264160..265020(-) (pilD) [Acinetobacter radioresistens strain MC-14]

ATGCAAGAATTAATTACCTTATTAAGTAATAACCCGACTGCGCTCTACATTGTGGTTGGGCTTTTCAGTCTATGTATTGG

CAGTTTTTTAAATGTCGTTATTCTTCGCACTCCTAAAATGATGGAGCAGGAATGGCGCAATGAATGTCAGTTGTTGCTTC

ATCCAGATCAGCCCCTAATTGATGAAACAAAACTCACGCTCAGCTATCCGGCTTCTACCTGTCCCAACTGTCATACGCCG

ATTCGCTGGTATCAGAATCTGCCTTTAGTCAGCTGGCTGCTACTCAGAGGCAAATGTGGTCAATGCCAGAATCCGATCAG

TATCCGTTACCCTCTAGTTGAACTTTTAACTGCTCTGTGTTCACTTATTGTAGTAGCTGTCTATGGCCCTACCCTGCAAA

TGCTATTTGGTCTGTTACTGACTTGGGCATTAATTACCCTGACCTTCATCGATTTTGATACCCAACTGCTTCCAGACCGG

TATACCTTGCCGCTTGCCGCCTTAGGCCTAGCTCTAAACAGTTATGCATTATATACCTCGGCAAGCTCTGCTATTTGGGG

TTATGTGATTGGGTTTTTATGCTTATGGATTGTGTATTACCTGTTTAAAGTCATCACCGGCAAAGAAGGCATGGGATACG

GGGACTTTAAGTTACTGGCCGCACTCGGAGCATGGATGGGTCCTTTAATGCTGCCTTTAATTGTGCTACTTTCATCTTTA

GTCGGGGCAATTATTGGTATCGTACTGCTCAAGATTCGCAAGGAAAACCAGCCTTTCGCCTTTGGTCCCTATATTGCGAT

TGCTGGCTGGATCGCTTTTTTATGGGGTGAGCAAATTATGAAAATTTATCTGGGAGGCTGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(139-247)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-126)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A062C1I7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilD	Acinetobacter nosocomialis M2	79.72	100	0.797
pilD	Acinetobacter baumannii D1279779	78.671	100	0.787
pilD	Vibrio cholerae strain A1552	53.875	94.755	0.51
pilD	Vibrio campbellii strain DS40M4	51.087	96.503	0.493
pilD	Neisseria gonorrhoeae MS11	47.312	97.552	0.462

Detailed information NTG0862625