Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Q4617_RS14035 Genome accession   NZ_CP131470
Coordinates   2862465..2864189 (+) Length   574 a.a.
NCBI ID   WP_112987173.1    Uniprot ID   A0A365PJY8
Organism   Acinetobacter junii strain SC22     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2857465..2869189
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q4617_RS14000 (Q4617_14000) nusA 2858317..2859801 (-) 1485 WP_112987097.1 transcription termination factor NusA -
  Q4617_RS14005 (Q4617_14005) rimP 2859839..2860363 (-) 525 WP_004916844.1 ribosome maturation factor RimP -
  Q4617_RS14025 (Q4617_14025) secG 2860986..2861315 (-) 330 WP_004916847.1 preprotein translocase subunit SecG -
  Q4617_RS14030 (Q4617_14030) tpiA 2861328..2862119 (-) 792 WP_112987172.1 triose-phosphate isomerase -
  Q4617_RS14035 (Q4617_14035) pilB 2862465..2864189 (+) 1725 WP_112987173.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Q4617_RS14040 (Q4617_14040) pilC 2864214..2865440 (+) 1227 WP_112987174.1 type II secretion system F family protein Machinery gene
  Q4617_RS14045 (Q4617_14045) pilD 2865440..2866300 (+) 861 WP_112987175.1 A24 family peptidase Machinery gene
  Q4617_RS14050 (Q4617_14050) coaE 2866302..2866901 (+) 600 WP_112987176.1 dephospho-CoA kinase -
  Q4617_RS14055 (Q4617_14055) - 2866898..2867809 (-) 912 WP_112987177.1 DMT family transporter -
  Q4617_RS14060 (Q4617_14060) rlmB 2867840..2868589 (-) 750 WP_004916859.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  Q4617_RS14065 (Q4617_14065) - 2868779..2869105 (-) 327 WP_004958605.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63675.84 Da        Isoelectric Point: 5.1355

>NTDB_id=862563 Q4617_RS14035 WP_112987173.1 2862465..2864189(+) (pilB) [Acinetobacter junii strain SC22]
MSGIHTSPKFSGFIRRIVDDGHIAASNMQSAIDAAKKAQQDIVAHLIEHHRLSPLTIAESISLEFGEPLFDLAVFDTALL
LREITDSKLIQKHRVVPLIQRGQILYVATSNPTNIDALDAIRFNTKLNIEPVIVEHNKLEKLIEQQFGDSGSFDFGENDD
FDLDNVDVASTDTNNTTEDDDTNHQDESPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIANPPLQLAT
RLASRLKVMSQMDISEKRMPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIEALGYEDEQKELFMSA
LDKPQGMLLITGPTGSGKTVSLYTGLNILNTEDTNISTAEDPVEINLEGINQVNVNPKTGLTFSAALKAFLRQDPDVIMV
GEIRDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSECKQPIEIPKQSLL
EMGFTEQDLANPDFQIFQPVGCDACREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAAASEKAGFNNLRRSGLKKVMQ
GITSLQEVNRVTSE

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=862563 Q4617_RS14035 WP_112987173.1 2862465..2864189(+) (pilB) [Acinetobacter junii strain SC22]
ATGTCAGGAATACATACGTCTCCAAAATTTTCGGGGTTTATTCGACGCATAGTCGATGATGGTCACATTGCTGCATCAAA
TATGCAGAGTGCGATTGATGCTGCAAAAAAAGCACAACAAGATATTGTTGCTCACTTAATTGAACATCATCGCCTATCAC
CTCTGACAATTGCGGAAAGCATCTCTTTAGAGTTTGGTGAGCCACTTTTTGATCTTGCTGTATTTGATACGGCTCTATTA
CTTCGTGAAATAACTGACAGCAAACTGATTCAAAAACATCGTGTTGTTCCACTCATACAACGTGGTCAGATTTTGTATGT
TGCGACCAGCAACCCGACGAATATTGATGCATTAGACGCAATCAGGTTCAATACCAAATTGAATATCGAACCTGTGATTG
TTGAGCACAATAAACTCGAAAAACTAATTGAACAGCAGTTTGGAGATAGTGGCAGTTTCGATTTTGGGGAAAATGACGAC
TTTGACTTAGATAATGTTGATGTTGCATCGACTGATACAAACAACACGACTGAGGATGATGATACAAACCATCAAGATGA
ATCACCTATCGTCAAATATATCAATAAACTATTGATTGATGCGATTCGTATGGGAGCCTCTGACTTACATTTTGAACCTT
ATGAAAAAATGTATCGCGTGCGTTATCGTGTGGATGGTGTACTCAGACAAATTGCAAACCCACCACTACAACTCGCAACT
CGTTTAGCGTCTCGTTTAAAAGTCATGTCTCAAATGGACATTTCTGAGAAGCGTATGCCTCAAGATGGTCGTATCAAACT
TAAACTATCTAAGACTAAAGCGATTGATTTTCGTGTTAACTCACTTCCAACTTTATTTGGAGAGAAACTGGTTCTTCGTA
TTTTAGATCCTTCAAGCGCAATGTTAGGTATTGAAGCATTAGGCTATGAGGATGAACAAAAAGAACTATTTATGAGTGCA
TTAGACAAGCCTCAGGGTATGCTTCTGATTACAGGTCCGACAGGTTCTGGTAAAACCGTATCGTTATATACAGGTCTAAA
TATCTTAAATACAGAAGATACCAATATTTCGACCGCAGAAGACCCAGTCGAAATCAACTTAGAAGGTATTAACCAAGTTA
ACGTCAACCCTAAAACAGGCTTAACTTTCTCAGCAGCATTAAAAGCATTTTTACGTCAAGACCCTGATGTCATCATGGTC
GGTGAGATTCGTGATCTCGAAACAGCTGAAATTGCAATTAAGGCAGCTCAAACAGGTCACATGGTGATGTCAACCCTACA
TACCAACAGCGCTCCTGAAACACTCACACGACTACGCAATATGGGGGTTCCCTCTTTCAATATTGCAACCTCGGTTAATC
TAGTGATTGCTCAACGTTTAGCCCGCCGCCTATGCTCGGAATGCAAACAACCTATCGAGATTCCTAAGCAAAGCCTTTTA
GAAATGGGCTTCACTGAACAAGATTTAGCCAACCCTGACTTTCAAATTTTTCAACCTGTCGGCTGCGATGCTTGTCGTGA
AGGATATAAAGGGCGTGTCGGTATTTATGAAGTAATGAAAGTGACACCCGAGATTTCCAAGATTATTATGGAAGATGGAA
ATGCACTACAAATTGCAGCAGCATCTGAAAAGGCTGGTTTCAATAATCTACGCCGTTCTGGTTTAAAAAAGGTCATGCAA
GGGATCACCTCTTTGCAAGAAGTTAACCGAGTAACAAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)

Type II/IV secretion system protein (T2SSE)

(196-463)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A365PJY8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.217

100

0.814
  pilB Acinetobacter baylyi ADP1

77.043

100

0.772
  pilB Legionella pneumophila strain ERS1305867

52.809

93.031

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

50

97.213

0.486
  pilB Vibrio cholerae strain A1552

49.278

96.516

0.476
  pilF Neisseria gonorrhoeae MS11

50.369

94.425

0.476
  pilB Vibrio campbellii strain DS40M4

51.644

90.07

0.465
  pilB/pilB1 Synechocystis sp. PCC 6803

34.477

100

0.368
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.764

93.031

0.361