Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Q5Z11_RS15455 Genome accession   NZ_CP130832
Coordinates   3458727..3460457 (-) Length   576 a.a.
NCBI ID   WP_303747214.1    Uniprot ID   -
Organism   Stenotrophomonas sp. 610A2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3453727..3465457
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q5Z11_RS15440 sucC 3454057..3455226 (-) 1170 WP_057629765.1 ADP-forming succinate--CoA ligase subunit beta -
  Q5Z11_RS15445 - 3455487..3457112 (+) 1626 WP_303747212.1 PAS domain-containing sensor histidine kinase -
  Q5Z11_RS15450 pilR 3457171..3458586 (+) 1416 WP_303747213.1 sigma-54 dependent transcriptional regulator Regulator
  Q5Z11_RS15455 pilB 3458727..3460457 (-) 1731 WP_303747214.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Q5Z11_RS15460 - 3460624..3461406 (-) 783 WP_303747215.1 sterol desaturase family protein -
  Q5Z11_RS20755 - 3461551..3461895 (-) 345 WP_405051613.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  Q5Z11_RS15470 - 3462350..3462449 (-) 100 Protein_3041 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  Q5Z11_RS15475 pilC 3462960..3464219 (+) 1260 WP_303747216.1 type II secretion system F family protein Machinery gene
  Q5Z11_RS15480 pilD 3464227..3465093 (+) 867 WP_303747217.1 A24 family peptidase Machinery gene

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62369.50 Da        Isoelectric Point: 5.5946

>NTDB_id=861501 Q5Z11_RS15455 WP_303747214.1 3458727..3460457(-) (pilB) [Stenotrophomonas sp. 610A2]
MNTAITANLVGITGIARRLVQDGALEESVARSAMAQAAEARVPLAQWLADKKLVSASHMAAANAMEFGMPLLDVSVFDTN
QSAMKLVSEELLRKHHVLPLFKRGGKLFVGTSNPTQQLDEIKFQTNLVVEPILVDEEQIRRTLDQWLASSDSLGDGLGGD
DEGMGDLDVSAGDEDMGGGGDTGVDAKGDDTPVVKFVNKALVDAIRKGASDIHFEPYEDDYRVRFRIDGLLKTVAKAPVK
LNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIDKLGYEPDQQKLF
LEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDDTRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDI
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCSNCKRPQQLPEN
ALLAEGFTAEQIAKGINLYEPVGCDECTEGYKGRTGIYQVMPMTDDIAAIVLQGGNAMDIAAAAQKIGINDLRQSALVKA
AQGTTSLAEINRVTKD

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=861501 Q5Z11_RS15455 WP_303747214.1 3458727..3460457(-) (pilB) [Stenotrophomonas sp. 610A2]
ATGAACACCGCCATCACCGCCAACCTGGTTGGAATCACCGGCATCGCCCGTCGCCTGGTCCAGGACGGAGCGCTGGAAGA
ATCGGTTGCACGTTCTGCGATGGCGCAAGCCGCCGAGGCCAGGGTGCCATTGGCGCAGTGGCTGGCCGACAAGAAGCTGG
TGAGCGCCTCGCACATGGCCGCTGCCAACGCCATGGAGTTCGGTATGCCGCTGCTGGATGTATCGGTCTTCGATACGAAC
CAGAGCGCGATGAAGCTGGTCAGCGAGGAATTGCTGCGCAAGCATCATGTGCTGCCGCTGTTCAAACGCGGCGGCAAGTT
GTTCGTCGGCACCAGCAATCCGACCCAGCAGCTCGACGAGATCAAGTTCCAGACCAACCTGGTGGTCGAGCCGATCCTGG
TTGACGAGGAGCAGATCCGCCGCACTTTGGACCAGTGGTTGGCCAGCAGCGACTCGTTGGGTGATGGCCTTGGTGGTGAC
GATGAAGGCATGGGAGACCTGGATGTCTCCGCCGGTGACGAGGACATGGGCGGCGGCGGCGACACTGGTGTTGATGCCAA
GGGTGACGACACGCCGGTGGTCAAGTTCGTCAACAAGGCCTTGGTGGATGCAATCCGCAAAGGCGCGTCGGATATCCACT
TCGAGCCCTACGAAGACGATTACCGCGTTCGTTTCCGTATCGATGGCCTGCTCAAGACCGTGGCCAAGGCACCGGTCAAG
CTGAATCAGCGTATTGCTGCGCGCCTCAAGGTCATGTCTCAGCTGGATATCGCCGAGAAGCGCGTGCCGCAGGATGGCCG
CATCAAGCTCAATCTGTCCAAGACCAAGCAGATCGACTTCCGCGTAAGTACCTTGCCAACCCTGTTCGGCGAAAAGATCG
TGCTGCGTATCCTCGACGGCAGCGCCGCCAAGCTGGGTATCGACAAGCTGGGTTATGAGCCGGACCAGCAGAAGCTGTTC
CTGGAAGCCATCCACAAGCCTTACGGGATGGTGCTGGTCACCGGTCCGACCGGCTCGGGTAAGACGGTGTCGCTGTATAC
CGCGCTGGGTATCCTCAATGACGATACCCGCAATATCTCCACGGCCGAGGATCCGGTGGAAATCCGCCTGCCGGGTGTGA
ACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTTGCGGCGGCGCTGCGTTCGTTCCTGCGACAGGATCCGGACATC
ATCATGGTCGGTGAAATCCGCGACCTTGAGACCGCGGAAATCGCGATCAAGGCCGCACAGACCGGTCACATGGTGCTGTC
CACGCTGCATACCAACGATGCGCCGCAGACCATCGCCCGCTTGATGAACATGGGCATCGCGCCCTACAACATCACATCTT
CGGTGACGCTGGTGATCGCACAGCGTCTGGCGCGGCGTCTGTGCAGCAATTGCAAGCGGCCGCAGCAGCTGCCGGAGAAC
GCGCTGCTGGCCGAAGGCTTTACCGCCGAGCAGATCGCCAAGGGCATAAACCTGTATGAGCCGGTGGGTTGCGATGAATG
CACCGAGGGCTACAAGGGTCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGATATCGCGGCGATCGTGCTGCAGG
GTGGCAATGCGATGGATATTGCTGCGGCCGCGCAGAAGATCGGTATCAACGATCTGCGGCAGTCGGCGTTGGTGAAGGCA
GCGCAGGGCACTACCAGCCTGGCTGAGATCAATCGCGTCACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(199-466)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

56.338

98.611

0.556
  pilB Acinetobacter baumannii D1279779

56.162

98.611

0.554
  pilB Legionella pneumophila strain ERS1305867

51.67

98.785

0.51
  pilF Neisseria gonorrhoeae MS11

50.263

99.132

0.498
  pilB Vibrio cholerae strain A1552

49.817

94.792

0.472
  pilB Vibrio campbellii strain DS40M4

46.127

98.611

0.455
  pilB Vibrio parahaemolyticus RIMD 2210633

46.454

97.917

0.455