Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Q5Z10_RS17025 Genome accession   NZ_CP130831
Coordinates   3709828..3711555 (-) Length   575 a.a.
NCBI ID   WP_303636556.1    Uniprot ID   -
Organism   Stenotrophomonas sp. 704A1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3704828..3716555
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q5Z10_RS17010 sucC 3705151..3706320 (-) 1170 WP_004154430.1 ADP-forming succinate--CoA ligase subunit beta -
  Q5Z10_RS17015 - 3706717..3708330 (+) 1614 WP_303636554.1 HAMP domain-containing sensor histidine kinase -
  Q5Z10_RS17020 pilR 3708385..3709758 (+) 1374 WP_303636555.1 sigma-54 dependent transcriptional regulator Regulator
  Q5Z10_RS17025 pilB 3709828..3711555 (-) 1728 WP_303636556.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Q5Z10_RS17030 - 3711620..3713734 (-) 2115 WP_303636557.1 sulfatase-like hydrolase/transferase -
  Q5Z10_RS17035 pilA 3713834..3714250 (-) 417 WP_303636558.1 pilin Machinery gene
  Q5Z10_RS17040 pilC 3714599..3715858 (+) 1260 WP_303639209.1 type II secretion system F family protein Machinery gene

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62054.99 Da        Isoelectric Point: 6.0405

>NTDB_id=861479 Q5Z10_RS17025 WP_303636556.1 3709828..3711555(-) (pilB) [Stenotrophomonas sp. 704A1]
MNTVTTANLVGITGLARRLVQDGALDEAAARDAMAKATAARQPLPTWFAQNKVVGAAQLAAANAVEFGMPLFDVSTFDSS
QNAMGLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHSLDEIKFHTNLVVEPILVDEDQIRRTLEQWQSSHDTLGDALGGD
DDGLGNLDVSGGDDDMAGGDSGIDAKGDDTPVVKFVNKVLIDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPVKL
NQRVAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKLFL
EAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDII
MVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCSNCKRPTDLPAHA
LLAEGFTQAQLDAGIQLHEAVGCDECTEGYKGRTGIYQVMPMTDEIATIVLAGGNALQIAEAAQALGVNDLRQSALKKVA
AGVTSLAEINRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=861479 Q5Z10_RS17025 WP_303636556.1 3709828..3711555(-) (pilB) [Stenotrophomonas sp. 704A1]
ATGAACACCGTAACCACCGCCAACCTCGTCGGCATCACCGGCCTGGCCCGCCGTCTGGTCCAGGATGGGGCCCTGGACGA
GGCCGCCGCCCGCGACGCCATGGCCAAGGCCACTGCCGCCCGCCAGCCGCTGCCGACCTGGTTCGCGCAGAACAAGGTGG
TCGGCGCCGCGCAGCTGGCGGCCGCCAATGCGGTCGAGTTCGGCATGCCGCTGTTCGATGTCTCCACGTTCGACAGCAGC
CAGAACGCGATGGGCCTGGTCAGCGAGGAACTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAAACGCGGCGGCAAGCT
GTTCGTGGGCACCAGCAACCCGACCCACTCGCTGGACGAGATCAAGTTCCATACCAATCTGGTGGTCGAGCCGATCCTGG
TCGATGAGGATCAGATCCGCCGCACCCTGGAGCAGTGGCAGTCCAGCCACGATACGCTCGGCGATGCGCTGGGGGGCGAC
GACGACGGCCTGGGCAACCTGGATGTCTCCGGCGGCGACGATGACATGGCCGGCGGCGACAGCGGCATCGACGCCAAGGG
CGACGACACCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGATCGACGCGATCCGCAAGGGCGCCTCGGACATCCACTTCG
AGCCCTACGAAGACGACTACCGCGTCCGCCTGCGCATCGACGGCCTGCTGAAGATGGTGGCGCGTGCCCCGGTGAAGTTG
AACCAGCGGGTGGCGGCGCGACTGAAGGTGATGGCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGACGGTCGCAT
CAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACGCTGCCGACGCTGTTCGGCGAGAAGGTGGTGC
TGCGTATCCTGGATGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTACGAGCCGGACCAGCAGAAGCTGTTCCTG
GAGGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGTCCGACCGGCTCGGGCAAGACGGTGTCGCTGTATACCGC
GCTGGGCATCCTCAACGACGAGACCCGCAACATCTCCACCGCCGAAGACCCGGTGGAAATCCGCCTGCCGGGCGTCAACC
AGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCTGCGGCGCTGCGTTCGTTCCTGCGCCAGGATCCGGACATCATC
ATGGTCGGCGAAATCCGTGACCTGGAGACGGCCGAGATCGCGATCAAGGCCGCCCAGACCGGTCACATGGTGCTGTCCAC
GCTGCACACCAACGATGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCGCCGTACAACATCACCAGCTCGG
TGACCCTGGTGATCGCCCAGCGCCTGGCCCGACGCCTGTGCTCGAACTGCAAGCGGCCGACCGATCTGCCGGCCCATGCG
CTGCTGGCCGAGGGCTTCACCCAGGCGCAGCTGGATGCCGGCATCCAGCTGCATGAAGCGGTGGGGTGCGACGAATGCAC
CGAGGGCTACAAGGGCCGTACCGGCATCTACCAGGTGATGCCGATGACCGACGAGATCGCCACGATCGTGCTGGCTGGCG
GCAACGCGCTGCAGATCGCCGAGGCCGCGCAGGCACTGGGCGTGAATGATCTGCGGCAGTCGGCCCTGAAGAAGGTGGCG
GCCGGCGTGACCAGCCTGGCCGAGATCAACCGCGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-151)

Type II/IV secretion system protein (T2SSE)

(198-465)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.842

99.13

0.563
  pilB Acinetobacter baylyi ADP1

56.79

98.609

0.56
  pilB Legionella pneumophila strain ERS1305867

51.664

99.304

0.513
  pilF Neisseria gonorrhoeae MS11

49.383

98.609

0.487
  pilB Vibrio cholerae strain A1552

48.772

99.13

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

45.739

100

0.457
  pilB Vibrio campbellii strain DS40M4

45.201

99.652

0.45