Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QYS58_RS11005 Genome accession   NZ_CP130143
Coordinates   2323521..2325335 (+) Length   604 a.a.
NCBI ID   WP_302138762.1    Uniprot ID   -
Organism   Halomonas alkalicola strain M2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2318521..2330335
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QYS58_RS10970 - 2318582..2318725 (+) 144 WP_302138748.1 hypothetical protein -
  QYS58_RS10975 - 2318824..2319249 (+) 426 WP_302138750.1 nucleotidyltransferase domain-containing protein -
  QYS58_RS10980 - 2319239..2319649 (+) 411 WP_302138753.1 DUF86 domain-containing protein -
  QYS58_RS10985 mscL 2319686..2320108 (-) 423 WP_302138755.1 large conductance mechanosensitive channel protein MscL -
  QYS58_RS10990 - 2320245..2320712 (-) 468 WP_302138757.1 hypothetical protein -
  QYS58_RS10995 - 2320967..2322640 (+) 1674 WP_302138759.1 PglL family O-oligosaccharyltransferase -
  QYS58_RS11000 - 2322703..2323260 (-) 558 WP_367185162.1 pilin -
  QYS58_RS11005 pilB 2323521..2325335 (+) 1815 WP_302138762.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QYS58_RS11010 pilC 2325388..2326623 (+) 1236 WP_302138764.1 type II secretion system F family protein Machinery gene
  QYS58_RS11015 pilD 2326631..2327485 (+) 855 WP_302143150.1 A24 family peptidase Machinery gene
  QYS58_RS11020 - 2327807..2328385 (-) 579 WP_119020348.1 hypoxanthine-guanine phosphoribosyltransferase -
  QYS58_RS11025 nagZ 2328431..2329438 (-) 1008 WP_302138769.1 beta-N-acetylhexosaminidase -
  QYS58_RS11030 - 2329583..2330257 (-) 675 WP_302138771.1 TetR/AcrR family transcriptional regulator -

Sequence

Protein


Download         Length: 604 a.a.        Molecular weight: 65596.18 Da        Isoelectric Point: 5.7889

>NTDB_id=857324 QYS58_RS11005 WP_302138762.1 2323521..2325335(+) (pilB) [Halomonas alkalicola strain M2]
MNDYRTPPSAPLPGSPYGSSQGSAPESTHLDATAGLRGLARRLVADGRISAAAAVRAEEEAREGELSLLEHVIDTGLVSA
RQATLSAAWEYGLPILDLDAIRPETLPRADAFPEKILRKLKVLPLMQRGHRLTVAVPYPSTLALLDELQFATGLTPEGVL
APVDQLGPVLESYLASSEKSMLDELKGVDDAIGELGFSGEEEDSGEVDPHAAANAANEDAPIIKFVNKILLDAIHRGASD
IHFEPYETSYRVRFRIDGMLHDVAHPPFAMRTRIAARLKVMSRLDISERRLPQDGAIKLRLSKTRSIDFRVNSLPTVYGE
KVVLRILDPASAQLGIEMLGFTPEQRAMYETVLDQPQGMILVTGPTGSGKTVTLYTGINILNEVERNICTAEDPVEIKVP
GVNQVNVIPKIGLDFASALRAFLRQDPDVVMVGEIRDLETAEIAVKASQTGHLVLSTVHTNSAAETLTRLANMGLAPFNI
ASSVSLIIAQRLARKLCKHCKQPADLPHEALAAQGFTETEIQQATFFEPVGCKHCTKGFKGRVGIYEVVPISHAMSQLIM
KNGNSMEFSDLARQEGYPDLRRSGLMKAMAGVTSLAEINRVTKD

Nucleotide


Download         Length: 1815 bp        

>NTDB_id=857324 QYS58_RS11005 WP_302138762.1 2323521..2325335(+) (pilB) [Halomonas alkalicola strain M2]
ATGAACGACTACCGCACCCCGCCGAGCGCCCCGCTCCCGGGCTCACCCTATGGCTCTTCACAGGGCTCGGCGCCGGAGAG
CACCCACCTTGATGCCACCGCGGGGCTGCGCGGCCTGGCGCGGCGCTTGGTGGCAGACGGGCGCATCAGCGCCGCCGCCG
CGGTGCGCGCAGAAGAAGAAGCTCGCGAGGGTGAGCTCTCGCTGCTGGAGCATGTGATCGACACCGGGCTGGTCTCCGCC
CGCCAGGCTACCCTCTCGGCGGCCTGGGAGTATGGCCTGCCGATCTTGGACCTCGACGCCATCCGCCCGGAGACACTGCC
CCGCGCCGATGCCTTCCCCGAGAAGATCCTGCGCAAGCTCAAGGTGCTGCCGCTGATGCAGCGCGGCCACCGTCTCACCG
TGGCGGTGCCCTACCCCTCGACGCTTGCCCTGCTCGACGAGCTGCAGTTCGCCACCGGCCTGACCCCGGAGGGGGTGCTG
GCCCCGGTGGACCAGCTCGGCCCGGTACTGGAGAGCTATCTCGCCTCCAGCGAGAAAAGCATGCTCGACGAGCTCAAGGG
GGTGGACGACGCCATCGGCGAGCTGGGCTTCTCTGGCGAGGAGGAGGACAGCGGCGAGGTGGACCCCCACGCCGCCGCCA
ACGCCGCCAACGAAGACGCGCCGATCATCAAGTTCGTCAACAAGATCCTGCTGGACGCCATCCACCGCGGCGCCTCCGAC
ATCCACTTCGAGCCCTATGAGACCAGCTACCGGGTGCGCTTTCGCATCGATGGCATGCTGCATGACGTGGCCCACCCGCC
CTTCGCCATGCGTACCCGCATCGCCGCGCGCCTCAAGGTGATGTCGCGGCTCGATATCTCGGAGCGCCGCCTGCCCCAGG
ATGGCGCCATCAAGCTCCGGCTCTCCAAGACCCGCTCCATCGATTTTCGCGTCAACTCGCTACCCACGGTCTATGGCGAG
AAGGTGGTGCTGCGGATCCTCGACCCCGCCTCGGCCCAGCTCGGCATCGAGATGCTCGGCTTCACGCCGGAGCAGCGGGC
GATGTACGAGACCGTGCTCGACCAGCCCCAGGGAATGATACTCGTCACCGGGCCCACCGGCAGCGGCAAGACGGTGACCC
TCTACACCGGCATCAATATCCTCAACGAGGTGGAGCGCAATATCTGCACCGCCGAGGACCCGGTGGAGATCAAGGTGCCC
GGGGTCAACCAGGTGAACGTGATCCCCAAGATCGGCCTGGACTTCGCCAGCGCCCTGCGCGCCTTCCTGCGCCAGGACCC
GGATGTGGTGATGGTGGGCGAGATCCGCGACCTGGAGACCGCCGAGATCGCGGTGAAGGCCTCCCAGACCGGCCACCTGG
TGCTCTCCACGGTGCACACCAACTCCGCCGCGGAGACCCTGACCCGCCTGGCCAACATGGGCCTGGCGCCGTTCAATATC
GCAAGCTCCGTGAGCCTGATCATCGCCCAGCGCCTGGCGCGCAAGCTCTGCAAGCACTGCAAGCAGCCGGCGGACCTGCC
CCATGAAGCGCTGGCCGCCCAGGGCTTCACGGAAACAGAGATCCAGCAGGCCACCTTCTTCGAACCGGTGGGCTGCAAGC
ACTGCACCAAGGGTTTCAAGGGCCGGGTGGGCATCTACGAGGTGGTGCCGATCAGCCATGCCATGAGCCAGCTGATCATG
AAGAACGGCAACTCCATGGAGTTCAGCGACCTGGCCCGCCAGGAGGGCTACCCGGACCTGCGCAGAAGCGGCCTGATGAA
GGCGATGGCCGGCGTAACGAGCCTGGCCGAGATCAACCGGGTGACCAAAGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(228-495)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(75-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

52.87

95.199

0.503
  pilB Acinetobacter baumannii D1279779

51.024

97.02

0.495
  pilB Legionella pneumophila strain ERS1305867

52.281

94.371

0.493
  pilB Vibrio cholerae strain A1552

49.721

88.907

0.442
  pilB Vibrio campbellii strain DS40M4

48.812

90.563

0.442
  pilB Vibrio parahaemolyticus RIMD 2210633

47.81

90.728

0.434
  pilF Neisseria gonorrhoeae MS11

45.184

94.536

0.427
  pilB/pilB1 Synechocystis sp. PCC 6803

39.781

90.728

0.361