Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QZL36_RS12700 Genome accession   NZ_CP129932
Coordinates   2770297..2771982 (+) Length   561 a.a.
NCBI ID   WP_308385410.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus isolate BM18B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2765297..2776982
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QZL36_RS12680 pdhR 2766879..2767646 (-) 768 WP_308385411.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  QZL36_RS12685 ampD 2768052..2768603 (-) 552 WP_025536234.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QZL36_RS12690 nadC 2768696..2769583 (+) 888 WP_025523107.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QZL36_RS12695 - 2769847..2770290 (+) 444 WP_149151214.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QZL36_RS12700 pilB 2770297..2771982 (+) 1686 WP_308385410.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QZL36_RS12705 pilC 2772006..2773229 (+) 1224 WP_308385409.1 type II secretion system F family protein Machinery gene
  QZL36_RS12710 pilD 2773294..2774163 (+) 870 WP_025533026.1 A24 family peptidase Machinery gene
  QZL36_RS12715 coaE 2774164..2774778 (+) 615 WP_005480887.1 dephospho-CoA kinase -
  QZL36_RS12720 zapD 2774806..2775546 (+) 741 WP_005480890.1 cell division protein ZapD -
  QZL36_RS12725 yacG 2775615..2775809 (+) 195 WP_005462546.1 DNA gyrase inhibitor YacG -
  QZL36_RS12730 rplS 2776104..2776457 (-) 354 WP_005462554.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62333.24 Da        Isoelectric Point: 5.5375

>NTDB_id=856439 QZL36_RS12700 WP_308385410.1 2770297..2771982(+) (pilB) [Vibrio parahaemolyticus isolate BM18B]
MLTNLSTILRQKGILTFSQEESLIEQVKASGISMPEALLSSGLFTSSELAEHLSSLFGLSQPELSQYEYASLCQQLGLRE
LITRHNALPLHRTPSTLLLAVADPTNQQAEDDFRFATGLQVELVLADFRQLSAAIRRLYGRAIGQERSQLKEINQEELAS
LVDVGADEIDNIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYEKMYRVRLRCDGILIETQQPPNHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSASLDIDKLGYSEQQKQLYLEALRRPQGMI
LMTGPTGSGKTVSLYTGLNILNKPEINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETIIRLSNMGVESFNLASSLSLIIAQRLARKLCPYCKQPQEHTVQLQHLGIQTTDN
IFKANPDGCNECTHGYSGRTGIYEVMRFDESLSEALIKGASVHELEKLAIANGMSTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=856439 QZL36_RS12700 WP_308385410.1 2770297..2771982(+) (pilB) [Vibrio parahaemolyticus isolate BM18B]
ATGTTAACAAACCTCTCAACCATTCTTCGTCAAAAAGGAATACTGACGTTTTCCCAAGAAGAATCGTTAATAGAGCAAGT
TAAAGCTTCTGGCATTTCGATGCCGGAAGCTTTACTTAGTTCTGGATTATTTACATCAAGCGAACTCGCTGAGCACTTAA
GCTCTCTCTTTGGTTTGAGTCAGCCCGAGTTATCTCAATATGAATATGCTTCGCTTTGCCAACAGCTCGGCCTACGTGAA
TTAATCACACGACATAATGCACTCCCACTCCACCGTACTCCTTCAACTTTATTATTAGCGGTTGCCGACCCCACCAATCA
ACAAGCTGAAGATGATTTCCGCTTTGCCACTGGTTTACAGGTTGAATTGGTGTTGGCGGATTTTCGTCAACTCAGTGCAG
CAATTCGACGCTTGTACGGGCGAGCCATCGGTCAGGAACGCTCCCAACTCAAAGAGATCAACCAAGAAGAGCTAGCGAGT
CTGGTGGATGTCGGTGCAGATGAAATCGACAATATCGAAGACTTGAGCCAAGACGAGTCGCCCGTTAGCCGCTACATCAA
CCAAATTCTGTTGGACGCGGTGCGTAAAGGCGCATCCGATATCCACTTTGAGCCTTATGAAAAGATGTACCGAGTTCGTC
TACGTTGCGATGGCATTTTGATCGAAACTCAGCAACCGCCAAATCACTTGAGTCGTCGCTTATCAGCTCGTATCAAAATT
CTCTCTAAACTTGATATTGCAGAGCGACGTTTACCACAAGATGGGCGAATTAAACTCAAACTGAATCAAGACACCGCGAT
TGATATGCGGGTATCTACGCTACCTACCTTGTTTGGAGAGAAGATCGTACTACGACTGCTCGATAGCAGCTCCGCATCAC
TGGATATCGACAAGCTTGGTTATAGTGAGCAACAAAAACAGCTGTATTTAGAGGCTCTGCGTCGTCCACAAGGTATGATT
CTGATGACAGGTCCAACAGGAAGCGGCAAAACCGTTTCGTTGTACACTGGGCTAAATATTCTCAATAAACCAGAGATCAA
CATCTCCACGGCAGAAGACCCAGTAGAAATTAACTTATCGGGCATTAACCAAGTTCAAGTCCAACCTAAGATTGGTTTTG
GCTTTGCAGAAGCACTACGTTCTTTTCTGCGTCAAGATCCCGATGTGGTCATGGTGGGTGAGATTCGAGACCTAGATACT
GCGGAAATCGCGATTAAGGCGTCCCAAACCGGTCACTTAGTGCTCTCTACTTTGCATACCAACTCTGCGGCTGAAACCAT
CATTCGTTTATCAAACATGGGCGTAGAAAGTTTTAACCTCGCCTCTTCTCTTAGTTTGATTATTGCCCAACGCCTAGCTC
GCAAACTTTGTCCATATTGTAAACAACCGCAAGAGCATACCGTTCAACTTCAGCACCTCGGCATTCAAACAACTGACAAC
ATCTTTAAAGCCAATCCAGATGGTTGCAACGAATGCACCCATGGTTATTCTGGCCGAACAGGTATCTATGAAGTCATGCG
CTTTGATGAATCTCTATCCGAAGCTCTTATTAAAGGAGCCTCGGTACATGAACTGGAAAAGCTTGCCATTGCGAACGGCA
TGAGCACCTTGCAAATGTCCGGAATAGAAAAACTCAAACAAGGCATCACAAGCTTTAGTGAGTTACAACGCGTGCTCTAT
TTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

97.504

100

0.975
  pilB Vibrio campbellii strain DS40M4

90.374

100

0.904
  pilB Vibrio cholerae strain A1552

74.021

100

0.742
  pilB Acinetobacter baumannii D1279779

51.711

93.761

0.485
  pilB Legionella pneumophila strain ERS1305867

49.165

96.078

0.472
  pilB Acinetobacter baylyi ADP1

50.87

92.157

0.469
  pilF Neisseria gonorrhoeae MS11

45.035

100

0.453
  pilB/pilB1 Synechocystis sp. PCC 6803

37.838

100

0.399
  pilF Thermus thermophilus HB27

36.525

100

0.367
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.155

100

0.365