Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QZJ86_RS02260 Genome accession   NZ_CP129884
Coordinates   475342..476496 (+) Length   384 a.a.
NCBI ID   WP_301936087.1    Uniprot ID   -
Organism   Methylomonas montana strain MW1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 470342..481496
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QZJ86_RS02235 (QZJ86_02235) argJ 471152..472366 (-) 1215 WP_301936078.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  QZJ86_RS02240 (QZJ86_02240) - 472891..473319 (-) 429 WP_301936081.1 hypothetical protein -
  QZJ86_RS02245 (QZJ86_02245) - 473310..473615 (-) 306 WP_301936083.1 hypothetical protein -
  QZJ86_RS02250 (QZJ86_02250) - 473647..473922 (-) 276 WP_301936085.1 sulfurtransferase TusA family protein -
  QZJ86_RS02255 (QZJ86_02255) - 473919..474962 (-) 1044 WP_301936086.1 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase -
  QZJ86_RS02260 (QZJ86_02260) pilU 475342..476496 (+) 1155 WP_301936087.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QZJ86_RS02265 (QZJ86_02265) - 476521..476844 (-) 324 WP_301936088.1 NGG1p interacting factor NIF3 -
  QZJ86_RS02270 (QZJ86_02270) - 476896..477045 (-) 150 WP_301936089.1 hypothetical protein -
  QZJ86_RS02275 (QZJ86_02275) lexA 477055..477684 (-) 630 WP_301936090.1 transcriptional repressor LexA -
  QZJ86_RS02280 (QZJ86_02280) - 477914..481225 (-) 3312 WP_301936091.1 diguanylate cyclase -

Sequence

Protein


Download         Length: 384 a.a.        Molecular weight: 42371.40 Da        Isoelectric Point: 6.0604

>NTDB_id=856192 QZJ86_RS02260 WP_301936087.1 475342..476496(+) (pilU) [Methylomonas montana strain MW1]
MEFKDYLKILVQHDGSDLYLTAGAPPAAKFQGQLKPLENIKLGHERIKEIADSIMDADQRASFEHVPEMNLAISEDGIGR
FRVNIFKQRNSYALVIRNIKVDIPNADVLGLPQILKDKIMEKRGLILFVGGTGSGKSTSLAALIDHRNSNSSGHIITIED
PIEYVHPHKKSLVNQREVGVDTLSYEDALKNTLRQAPDVILIGEIRSQETMEHALAFAETGHLCLSTLHANNANQALDRI
INFFPEERRNQLLLDLSLNLQAFISQRLVPTIEGKRVAAIEILLGTKLVSDLIQKGEVHAIKEAMEKSEAIGMQTFDSHL
LKLYKGGVISLEEALRNSDSPNNLKLKINLSEGLGSATKGGNLGASLSLQEISKKEGEEDEEGH

Nucleotide


Download         Length: 1155 bp        

>NTDB_id=856192 QZJ86_RS02260 WP_301936087.1 475342..476496(+) (pilU) [Methylomonas montana strain MW1]
ATGGAATTTAAAGATTATCTAAAAATCCTGGTTCAGCACGACGGTTCCGATCTTTATCTGACGGCCGGCGCACCACCGGC
GGCTAAGTTTCAAGGCCAATTAAAGCCATTGGAAAATATTAAACTCGGCCACGAACGCATCAAGGAAATCGCCGACAGCA
TCATGGATGCCGATCAACGCGCCAGCTTCGAGCATGTGCCGGAAATGAATCTCGCCATTTCGGAAGACGGCATTGGCCGG
TTTCGGGTCAATATCTTCAAACAACGCAATTCTTACGCATTAGTGATTCGCAACATCAAGGTTGATATTCCTAATGCCGA
TGTGCTGGGTTTGCCGCAAATTCTGAAAGACAAAATCATGGAAAAGCGCGGCCTGATTTTGTTCGTCGGCGGTACCGGTT
CGGGTAAGTCGACCTCGCTAGCGGCCTTGATCGACCATCGCAACAGCAACTCGTCCGGCCATATCATTACTATCGAAGAT
CCGATCGAATACGTGCATCCGCATAAAAAGTCGCTGGTCAATCAGCGCGAGGTGGGTGTCGATACCCTCAGCTATGAAGA
TGCGTTGAAAAATACCTTGCGGCAGGCGCCGGATGTGATTTTGATCGGCGAAATTCGTAGCCAGGAAACCATGGAACATG
CGTTGGCTTTTGCCGAAACCGGACATTTATGTTTGTCGACCTTGCATGCCAATAACGCTAATCAGGCCTTGGACCGTATC
ATCAACTTTTTTCCGGAGGAGCGCCGCAATCAGTTGTTGCTGGATTTGTCACTTAATTTGCAAGCCTTTATTTCACAACG
CCTGGTGCCGACCATCGAAGGCAAGCGCGTGGCGGCGATTGAAATTTTGCTGGGCACCAAGCTGGTCAGCGATCTGATTC
AGAAAGGCGAGGTTCACGCCATCAAGGAAGCGATGGAAAAATCGGAAGCCATCGGCATGCAAACCTTCGATAGCCATTTA
CTGAAACTTTACAAAGGCGGCGTGATTTCCCTGGAGGAAGCGTTGCGTAATTCTGATTCGCCGAATAACCTGAAACTGAA
AATCAACTTGAGCGAGGGCTTGGGATCAGCAACCAAGGGCGGTAACCTCGGCGCGAGCCTCTCCTTGCAGGAAATCAGCA
AGAAAGAAGGCGAAGAAGACGAGGAAGGCCACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(80-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.143

94.792

0.542
  pilU Acinetobacter baylyi ADP1

55.085

92.188

0.508
  pilU Vibrio cholerae strain A1552

48.794

97.135

0.474
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.111

93.75

0.385
  pilT Pseudomonas aeruginosa PAK

40.698

89.583

0.365
  pilT Legionella pneumophila strain Lp02

42.424

85.938

0.365
  pilT Legionella pneumophila strain ERS1305867

42.424

85.938

0.365