Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   V6S33_RS03660 Genome accession   NZ_CP145679
Coordinates   769634..771019 (-) Length   461 a.a.
NCBI ID   WP_001025194.1    Uniprot ID   -
Organism   Escherichia coli strain S1-NGR-02-A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 764634..776019
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6S33_RS03625 (V6S33_03625) mutT 764964..765353 (+) 390 WP_000736009.1 8-oxo-dGTP diphosphatase MutT -
  V6S33_RS03630 (V6S33_03630) yacG 765569..765766 (-) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  V6S33_RS03635 (V6S33_03635) zapD 765776..766519 (-) 744 WP_001194734.1 cell division protein ZapD -
  V6S33_RS03640 (V6S33_03640) coaE 766519..767139 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  V6S33_RS03645 (V6S33_03645) - 767164..767208 (+) 45 WP_120795372.1 protein YacM -
  V6S33_RS03650 (V6S33_03650) guaC 767364..768407 (+) 1044 WP_001217338.1 GMP reductase -
  V6S33_RS03655 (V6S33_03655) hofC 768442..769644 (-) 1203 WP_000157266.1 protein transport protein HofC -
  V6S33_RS03660 (V6S33_03660) pilB 769634..771019 (-) 1386 WP_001025194.1 type II secretion system protein GspE Machinery gene
  V6S33_RS03665 (V6S33_03665) ppdD 771029..771469 (-) 441 WP_000360900.1 prepilin peptidase-dependent pilin -
  V6S33_RS03670 (V6S33_03670) nadC 771672..772565 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  V6S33_RS03675 (V6S33_03675) ampD 772653..773204 (+) 552 WP_000923710.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  V6S33_RS03680 (V6S33_03680) ampE 773201..774055 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  V6S33_RS03685 (V6S33_03685) aroP 774098..775471 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50665.38 Da        Isoelectric Point: 6.5396

>NTDB_id=852073 V6S33_RS03660 WP_001025194.1 769634..771019(-) (pilB) [Escherichia coli strain S1-NGR-02-A]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCIHCYHVFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=852073 V6S33_RS03660 WP_001025194.1 769634..771019(-) (pilB) [Escherichia coli strain S1-NGR-02-A]
ATGAATATTCCACAGCTCACGGCCCTGTGCCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAAGTGGTTCATGT
TGCGGTGGTCGATGCCCCCTCACATGAGTTGCTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGCCAGGCACTGGATGTCAACACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGACTCACCTTTCAGGGCGTGTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACAGCAGAGATCGCTATTAAAGCGGCGCAAACTGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGGGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCACATTGTCGCCGGCAGCAAGGGGAGCCCATCCACATTCCAGACAATGTATGGC
CATCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTATACATTGCTACCACGTTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTGCCCATAACGCCGGTCATTCGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCACGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTAGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TACTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(88-353)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

49.74

83.297

0.414
  pilB Glaesserella parasuis strain SC1401

41.037

100

0.412
  pilB Acinetobacter baylyi ADP1

40.171

100

0.408
  pilB Vibrio campbellii strain DS40M4

48.187

83.731

0.403
  pilB Vibrio cholerae strain A1552

46.41

84.599

0.393
  pilB Vibrio parahaemolyticus RIMD 2210633

46.154

84.599

0.39
  pilB Haemophilus influenzae 86-028NP

44.961

83.948

0.377
  pilB Acinetobacter baumannii D1279779

43.655

85.466

0.373
  pilF Neisseria gonorrhoeae MS11

44.416

83.514

0.371
  pilB Haemophilus influenzae Rd KW20

44.186

83.948

0.371
  pilF Thermus thermophilus HB27

40.587

88.72

0.36