Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PSR66_RS30485 Genome accession   NZ_CP128834
Coordinates   6731444..6732661 (-) Length   405 a.a.
NCBI ID   WP_273864768.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis strain PcR3-3(2)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6726444..6737661
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSR66_RS30455 (PSR66_30455) - 6728310..6728777 (+) 468 WP_016703672.1 hypothetical protein -
  PSR66_RS30460 (PSR66_30460) - 6728774..6729463 (+) 690 WP_273864767.1 energy-coupling factor ABC transporter permease -
  PSR66_RS30465 (PSR66_30465) - 6729529..6729744 (+) 216 WP_009050911.1 hypothetical protein -
  PSR66_RS30470 (PSR66_30470) yacG 6729749..6729952 (-) 204 WP_009045670.1 DNA gyrase inhibitor YacG -
  PSR66_RS30475 (PSR66_30475) coaE 6729949..6730572 (-) 624 WP_009045669.1 dephospho-CoA kinase -
  PSR66_RS30480 (PSR66_30480) pilD 6730569..6731441 (-) 873 WP_009050909.1 A24 family peptidase Machinery gene
  PSR66_RS30485 (PSR66_30485) pilC 6731444..6732661 (-) 1218 WP_273864768.1 type II secretion system F family protein Machinery gene
  PSR66_RS30490 (PSR66_30490) - 6733026..6733442 (+) 417 WP_009050907.1 pilin -
  PSR66_RS30495 (PSR66_30495) - 6733730..6734086 (+) 357 WP_009050906.1 BON domain-containing protein -
  PSR66_RS30500 (PSR66_30500) - 6734289..6737228 (-) 2940 WP_273864769.1 DUF748 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44435.22 Da        Isoelectric Point: 10.4090

>NTDB_id=849712 PSR66_RS30485 WP_273864768.1 6731444..6732661(-) (pilC) [Pseudomonas chlororaphis strain PcR3-3(2)]
MAVKAAKVSVYLWEGTDRQGSKLRGELSGHTPSLIRAQLRKQGISPARVRKKSSSLFQRTPPIKARDISLFTRQLATLIK
AGVPLLQSFDIIAEGFDNPNMRKLLGELKQDIAAGSSFTSALRKRPRYFDELYCSLVDAGEQAGALETLLERVATYKEKS
ENLKTRIRKAMTYPLAVICVALVVTGILLIKVVPQFQSMFQGFGAQLPVFTQLVIALSEFVQHWWWLLAGGLLLSVFGLR
HAHRRSPRFRQWLDVGLLKLPLVAPLLYKSAVARYARTLSTTFAAGVPLVEALGSVAGATGNQVFKHAVNRIRQDVATGM
QLHFSMRASGIFPGMAIQMTAIGEESGALDGMLEKVAIHYEEEVDTLVDTLSSLMEPVIMVVLGTLVGGLVVAMYLPIFQ
LGTAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=849712 PSR66_RS30485 WP_273864768.1 6731444..6732661(-) (pilC) [Pseudomonas chlororaphis strain PcR3-3(2)]
ATGGCGGTCAAAGCAGCAAAAGTCAGTGTTTACCTGTGGGAAGGCACCGACAGGCAAGGCAGCAAACTGCGCGGCGAATT
GAGCGGCCATACCCCCTCGCTGATCAGGGCCCAGTTGCGCAAGCAAGGCATCAGCCCGGCCAGGGTCAGGAAGAAATCCA
GCTCGCTGTTCCAGCGCACGCCCCCGATCAAGGCTCGCGACATCAGCCTGTTCACCCGGCAGTTGGCGACCCTGATCAAG
GCCGGCGTACCGCTGCTGCAATCGTTCGACATCATTGCCGAGGGCTTCGATAACCCGAACATGCGCAAACTGCTGGGCGA
GCTGAAACAGGACATCGCCGCCGGCAGCAGCTTCACCAGCGCACTACGCAAGAGGCCGCGGTATTTCGACGAGCTGTACT
GCAGCCTGGTGGATGCCGGCGAGCAGGCCGGCGCCCTGGAAACCCTGCTGGAGCGGGTGGCGACCTACAAGGAAAAAAGC
GAAAACCTCAAGACCCGAATCCGCAAGGCCATGACCTATCCCCTGGCGGTGATCTGCGTGGCGCTGGTGGTGACAGGGAT
CCTGCTGATCAAGGTGGTGCCGCAATTCCAGTCGATGTTCCAAGGCTTCGGCGCGCAACTGCCGGTGTTCACCCAACTGG
TCATCGCCCTCTCGGAGTTCGTGCAGCACTGGTGGTGGCTGTTGGCGGGCGGTCTGTTGCTCAGCGTATTCGGCCTGCGC
CATGCCCACCGGCGCTCACCGCGCTTTCGCCAGTGGCTGGACGTGGGCCTGCTGAAACTGCCGTTGGTAGCGCCGCTGCT
GTACAAGTCCGCGGTGGCCCGCTACGCCCGCACCTTGTCCACCACCTTCGCCGCCGGGGTACCACTGGTGGAGGCCCTGG
GCTCGGTGGCCGGGGCCACTGGCAACCAGGTGTTCAAGCATGCGGTGAACCGCATCAGGCAGGACGTGGCGACTGGCATG
CAGTTGCATTTTTCCATGCGCGCATCCGGCATTTTTCCCGGCATGGCGATCCAGATGACCGCCATCGGCGAAGAATCCGG
GGCCCTGGATGGCATGCTGGAAAAAGTCGCGATCCACTACGAGGAAGAGGTCGATACCCTGGTCGACACCCTGAGCAGTC
TCATGGAGCCCGTGATCATGGTAGTTTTGGGCACCCTCGTCGGCGGCCTGGTGGTCGCCATGTACTTACCCATCTTCCAA
CTCGGCACAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

66.42

100

0.664
  pilC Acinetobacter baylyi ADP1

55.637

100

0.56
  pilC Acinetobacter baumannii D1279779

54.878

100

0.556
  pilC Legionella pneumophila strain ERS1305867

53.03

97.778

0.519
  pilG Neisseria gonorrhoeae MS11

42.68

99.506

0.425
  pilG Neisseria meningitidis 44/76-A

41.935

99.506

0.417
  pilC Vibrio cholerae strain A1552

42.092

96.79

0.407
  pilC Vibrio campbellii strain DS40M4

39.552

99.259

0.393