Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   JUH08_RS02780 Genome accession   NZ_AP024354
Coordinates   611578..613650 (+) Length   690 a.a.
NCBI ID   WP_203790401.1    Uniprot ID   -
Organism   Aquipseudomonas alcaligenes strain KAM426     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 606578..618650
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JUH08_RS02760 (KAM426_05580) pilM 608682..609746 (+) 1065 WP_061903206.1 pilus assembly protein PilM Machinery gene
  JUH08_RS02765 (KAM426_05590) - 609746..610372 (+) 627 WP_203790385.1 PilN domain-containing protein -
  JUH08_RS02770 (KAM426_05600) pilO 610369..611001 (+) 633 WP_203790394.1 type 4a pilus biogenesis protein PilO -
  JUH08_RS02775 (KAM426_05610) pilP 610998..611525 (+) 528 WP_021698831.1 type 4a pilus biogenesis lipoprotein PilP -
  JUH08_RS02780 (KAM426_05620) pilQ 611578..613650 (+) 2073 WP_203790401.1 type IV pilus secretin PilQ Machinery gene
  JUH08_RS02785 (KAM426_05630) aroK 613653..614171 (+) 519 WP_021698829.1 shikimate kinase AroK -
  JUH08_RS02790 (KAM426_05640) aroB 614221..615324 (+) 1104 WP_203790409.1 3-dehydroquinate synthase -
  JUH08_RS02795 (KAM426_05650) - 615334..616986 (+) 1653 WP_203790417.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74372.19 Da        Isoelectric Point: 4.6218

>NTDB_id=84929 JUH08_RS02780 WP_203790401.1 611578..613650(+) (pilQ) [Aquipseudomonas alcaligenes strain KAM426]
MNSALSRVAISLISGFIASAAVAADLSGLDVAALPGDRVELKLQFDSPVVAPRGYTIEQPARIALDLPGVANKLGVKNRE
LGVGNARSVTIVETKDRTRLIVNLSTLVPYATRAEGNNLFVVVGEAATSASNAVATPAQSLTKSVPVSGRAIQGVDFQRG
EEGEGNVVIALSDSSVTADIQEQGGKIQLSFPKAALPDSLKVRLDVKDFATPVQYVSASAQADKVNISIEPQGFYDYLVF
QADNKLTVSVKPLSSDDVEKRKLERFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLRLQNVPWDQAL
DLVLKTRGLDKRKVGNVLLIAPADEIAARERQELEAQNQIAELAPLRRELIQVNYAKASDIAALFQSVAGIGGEGAKEER
GSVTVDDRTNSIIAYQTQDKLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKELGVRWGGSTNLQGDKNWTVYGNDDRG
DEGGRTGATQGPNVPFVDLGAAGRTSGIGIGFVTDNVLLDLELSAMEKTGNGEVISQPKVVTSDKETAKILKGTEVPYEE
SSSSGATTIAFKEAALSLEVTPQITPDNRIIMEVKVNKDEPDYQNALNNVPPIRKNEVNAKVLVNDGETIVIGGVFSNEQ
SKAVDKVPLLGDMPFVGRLFRRDVVKDTKNELLVFITPRIMNNQAIAVSQ

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=84929 JUH08_RS02780 WP_203790401.1 611578..613650(+) (pilQ) [Aquipseudomonas alcaligenes strain KAM426]
ATGAATAGCGCCCTCTCTCGCGTTGCCATCTCATTGATTTCAGGGTTTATTGCTTCGGCTGCAGTAGCAGCAGACCTCAG
TGGATTGGATGTGGCTGCGCTCCCTGGTGATAGGGTTGAGCTTAAGTTGCAGTTTGACAGCCCCGTGGTGGCTCCGCGTG
GCTATACGATAGAGCAGCCCGCGCGCATTGCTTTGGATCTGCCCGGTGTGGCAAACAAGCTGGGTGTTAAGAATCGTGAG
CTTGGTGTGGGAAATGCGCGAAGCGTGACCATCGTCGAGACAAAGGATCGGACCCGTCTGATAGTTAACTTGTCAACTCT
GGTCCCCTACGCGACTAGGGCTGAGGGTAACAATCTATTTGTGGTTGTGGGTGAGGCTGCGACTTCAGCTTCCAATGCGG
TTGCCACTCCAGCGCAATCGTTGACTAAATCTGTCCCTGTCTCCGGTCGAGCGATCCAAGGTGTTGATTTTCAACGTGGT
GAGGAAGGCGAAGGGAATGTCGTCATTGCTTTGTCGGACTCCTCGGTGACGGCCGATATTCAGGAGCAAGGCGGGAAAAT
CCAGCTGTCCTTTCCTAAAGCTGCCCTCCCTGACAGCCTCAAGGTTCGTCTCGATGTGAAGGATTTTGCGACGCCTGTTC
AATATGTGAGTGCGTCTGCCCAGGCTGATAAGGTAAATATAAGTATTGAGCCTCAGGGTTTTTACGATTACTTGGTCTTC
CAGGCGGATAACAAGCTGACAGTCAGTGTTAAGCCCCTGTCTTCTGATGATGTCGAGAAGCGTAAGCTTGAGCGTTTTGC
TTACACCGGTGAGAAGCTTTCTCTTAATTTTCAGGATATTGATGTTCGATCTGTGTTACAGCTGATTGCTGACTTCACTG
ATTTGAATCTGGTGGCGAGTGATACTGTTCAGGGTAACATTACCCTGCGGCTGCAAAATGTGCCGTGGGACCAAGCTTTG
GATCTTGTGCTGAAGACGCGTGGCTTGGACAAGAGGAAGGTTGGCAACGTATTGCTTATTGCTCCCGCGGATGAGATAGC
GGCACGTGAGCGTCAGGAGTTGGAGGCGCAAAATCAAATTGCAGAGCTTGCGCCGCTGCGTCGTGAGCTGATTCAGGTGA
ATTATGCTAAGGCATCTGATATTGCAGCTTTGTTCCAATCTGTTGCGGGTATCGGTGGTGAAGGCGCTAAGGAGGAGAGG
GGTTCGGTTACAGTGGATGATCGAACTAACAGTATTATTGCTTATCAAACACAGGATAAATTAGATGAGTTGCGACGTAT
TGTTGCTCAGCTTGATATTCCTGTGCGGCAGGTAATGATTGAGGCGCGAATTGTTGAGGCCAATGTTGACTATGACAAGG
AGCTCGGCGTTCGTTGGGGCGGCAGCACCAATCTGCAGGGTGATAAGAACTGGACTGTCTATGGTAATGACGATCGCGGT
GACGAGGGCGGGCGCACTGGTGCAACCCAAGGGCCCAACGTTCCTTTTGTTGATCTCGGCGCTGCTGGTCGTACCTCTGG
TATCGGTATTGGCTTCGTAACAGACAATGTTTTACTGGATCTCGAGTTGTCTGCTATGGAGAAGACCGGCAACGGTGAGG
TCATCTCGCAACCTAAGGTGGTCACCTCCGATAAGGAAACCGCGAAGATCCTGAAGGGTACTGAGGTTCCCTACGAGGAG
TCGAGCTCTAGCGGTGCTACGACTATTGCATTCAAAGAGGCAGCGCTGTCTCTTGAAGTGACCCCGCAGATTACTCCTGA
TAACCGCATTATCATGGAGGTAAAGGTCAACAAGGATGAGCCGGATTATCAGAACGCTCTGAACAACGTACCTCCCATCA
GGAAGAATGAGGTCAATGCAAAAGTCCTCGTTAATGATGGTGAGACAATAGTTATCGGCGGCGTCTTCTCGAATGAGCAG
TCCAAGGCCGTTGACAAGGTGCCGCTCTTGGGTGACATGCCTTTTGTCGGTCGCCTGTTCCGTCGTGACGTGGTAAAGGA
TACGAAGAACGAGCTGCTGGTCTTCATCACTCCGCGGATCATGAATAACCAGGCCATTGCGGTAAGCCAGTAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(296-342)

Bacterial type II/III secretion system short domain (Secretin_N)

(370-437)

Bacterial type II and III secretion system protein (Secretin)

(525-681)

AMIN domain (AMIN)

(38-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.259

100

0.762
  pilQ Acinetobacter baumannii D1279779

39.782

100

0.423
  pilQ Acinetobacter baumannii strain A118

39.782

100

0.423
  comQ Acinetobacter baylyi ADP1

41.714

100

0.423
  pilQ Legionella pneumophila strain Lp02

39.586

98.116

0.388
  pilQ Legionella pneumophila strain ERS1305867

39.439

98.116

0.387

Multiple sequence alignment