Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SrhCFBP13503_RS04890 Genome accession   NZ_CP128598
Coordinates   1080740..1081885 (+) Length   381 a.a.
NCBI ID   WP_141057346.1    Uniprot ID   -
Organism   Stenotrophomonas rhizophila strain CFBP 13503     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1075740..1086885
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SrhCFBP13503_RS04865 (SrhCFBP13503_04860) - 1076266..1077075 (-) 810 WP_392732559.1 short chain dehydrogenase -
  SrhCFBP13503_RS04870 (SrhCFBP13503_04865) - 1076966..1077847 (+) 882 WP_141057348.1 LysR substrate-binding domain-containing protein -
  SrhCFBP13503_RS04875 (SrhCFBP13503_04870) proC 1078004..1078825 (-) 822 WP_141057347.1 pyrroline-5-carboxylate reductase -
  SrhCFBP13503_RS04880 (SrhCFBP13503_04875) - 1078857..1079537 (-) 681 WP_181406136.1 YggS family pyridoxal phosphate-dependent enzyme -
  SrhCFBP13503_RS04885 (SrhCFBP13503_04880) pilT 1079624..1080661 (+) 1038 WP_070208322.1 type IV pilus twitching motility protein PilT Machinery gene
  SrhCFBP13503_RS04890 (SrhCFBP13503_04885) pilU 1080740..1081885 (+) 1146 WP_141057346.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SrhCFBP13503_RS04895 (SrhCFBP13503_04890) - 1082064..1082642 (+) 579 WP_181406135.1 DUF4234 domain-containing protein -
  SrhCFBP13503_RS04900 (SrhCFBP13503_04895) - 1082780..1083763 (+) 984 WP_141057344.1 aldo/keto reductase -
  SrhCFBP13503_RS04905 (SrhCFBP13503_04900) - 1084095..1084916 (-) 822 WP_141057343.1 alpha/beta fold hydrolase -
  SrhCFBP13503_RS04910 (SrhCFBP13503_04905) - 1085087..1085305 (+) 219 WP_038690009.1 DUF4287 domain-containing protein -
  SrhCFBP13503_RS04915 (SrhCFBP13503_04910) - 1085370..1086071 (+) 702 WP_141057342.1 hypothetical protein -
  SrhCFBP13503_RS04920 (SrhCFBP13503_04915) - 1086140..1086847 (+) 708 WP_038690005.1 YitT family protein -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42495.87 Da        Isoelectric Point: 6.8287

>NTDB_id=849242 SrhCFBP13503_RS04890 WP_141057346.1 1080740..1081885(+) (pilU) [Stenotrophomonas rhizophila strain CFBP 13503]
MNTTTTTTIDFTSFLKLMAHQKASDLFITAGMPPAIKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFA
IGLSGVGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNHNST
GHIITIEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANN
ANQAMDRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPSPDGKSRKVAMEIMLGTPLVQDYIRDGEIHKLKEVMKESVQL
GMRTFDQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEVR

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=849242 SrhCFBP13503_RS04890 WP_141057346.1 1080740..1081885(+) (pilU) [Stenotrophomonas rhizophila strain CFBP 13503]
ATGAACACCACCACCACCACGACCATCGACTTCACCTCGTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGACCTGTT
CATCACCGCGGGCATGCCGCCGGCGATCAAGGTCAACGGCAAGATCTCGCCGATCACCCAGACCCCGCTGACACCGCAGC
AGAGCCGCGACCTGGTGCTCAACGTGATGACGCCGGCGCAGCGCGAAGAGTTCGAAAAGACCCACGAGTGCAACTTCGCC
ATCGGCCTGTCCGGCGTCGGCCGGTTCCGCGTGAGCTGCTTCTACCAGCGCAACCAGGTGGGCATGGTGCTGCGTCGCAT
CGAGACGCGCATTCCCACCGTGGAAGAGCTGAGCCTGCCGCCGATCATCAAGACGCTGGCGATGACCAAGCGCGGCATCA
TCCTGTTCGTGGGCGCTACCGGTACCGGTAAATCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCACAACTCGACC
GGGCACATCATCACCATCGAAGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTGGG
TATCGATACCGACAGCTGGGAAGCGGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGC
GTACCCGCGAGGGCATGGACCACGCCATCGCCTTCGCCGAAACCGGCCACCTGGTGCTGTGCACGCTGCACGCCAACAAC
GCCAACCAGGCGATGGACCGCATCATCAACTTCTTCCCCGAAGACCGGCGCAACCAGTTGCTGATGGACCTGTCGCTGAA
CCTCAAGGGCGTGGTGGCGCAGCAGCTGATTCCCTCGCCGGACGGCAAGTCGCGCAAGGTGGCGATGGAGATCATGCTGG
GCACGCCGCTGGTGCAGGACTACATCCGCGATGGCGAGATCCACAAGCTCAAGGAAGTGATGAAGGAGTCGGTGCAGCTG
GGCATGCGTACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCCGGTGAGATCAGCTACGAAGACGCGCTGCGTTACGC
CGATTCGCAGAACGAAGTGCGCCTGCGCATCAAGCTGTCGCAGGGCGGCGATGCACGCACGCTGTCGCAGGGCCTGGACG
GAGTGGAGATCTCCGAGGTCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(123-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

96.325

0.651
  pilU Acinetobacter baylyi ADP1

63.866

93.701

0.598
  pilU Vibrio cholerae strain A1552

53.371

93.438

0.499
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.458

93.963

0.399
  pilT Acinetobacter nosocomialis M2

40.407

90.289

0.365
  pilT Acinetobacter baumannii D1279779

40.407

90.289

0.365
  pilT Acinetobacter baumannii strain A118

40.407

90.289

0.365