Detailed information NTG0848519

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: V5G07_RS10240
• Genome accession: NZ_CP145015
• Coordinates: 1946110..1947336 (-)
• Length: 408 a.a.
• NCBI ID: WP_003697786.1
• Uniprot ID: B4RPS5
• Organism: Neisseria gonorrhoeae strain WHO_Z_2024
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1941110..1952336

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V5G07_RS10230 (V5G07_10250)	-	1943079..1943363 (+)	285	WP_003692093.1	GIY-YIG nuclease family protein	-
V5G07_RS10235 (V5G07_10255)	yccS	1943843..1945993 (-)	2151	WP_003699137.1	YccS family putative transporter	-
V5G07_RS10240 (V5G07_10260)	pilU	1946110..1947336 (-)	1227	WP_003697786.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
V5G07_RS10245 (V5G07_10265)	pilT	1947500..1948543 (-)	1044	WP_003690200.1	type IV pilus twitching motility protein PilT	Machinery gene
V5G07_RS10250 (V5G07_10270)	-	1948663..1949349 (+)	687	WP_003690201.1	YggS family pyridoxal phosphate-dependent enzyme	-
V5G07_RS10255 (V5G07_10275)	-	1949349..1949756 (+)	408	WP_003701818.1	hypothetical protein	-
V5G07_RS10260 (V5G07_10280)	proC	1949805..1950596 (+)	792	WP_003692105.1	pyrroline-5-carboxylate reductase	-
V5G07_RS10265 (V5G07_10285)	dksA	1950745..1951161 (+)	417	WP_003692108.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 408 a.a.
• Molecular weight: 45668.30 Da
• Isoelectric Point: 7.2909

>NTDB_id=848519 V5G07_RS10240 WP_003697786.1 1946110..1947336(-) (pilU) [Neisseria gonorrhoeae strain WHO_Z_2024]
MNTDNLHDILDETVQVYSQKKQSRSETPAEIGAHFHPLLDRLCETAEAQNASDILISKGFPPSLKINSALTPQPQKALTG
EETAAIAASTMNAEQSEIFRRDGEINYSVQSRSGTRYRANAYYSQGSAGLVLRRINHVIPQMRELGLPEKLKDLAVAPRG
LLIIVGPTGSGKSTTMATMLEHRNKTLPGHIVTIEDPIEFIYKPRRCIFTQREIGVDTINWQTAVQNAMRQSPDVVCIGE
VRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERILNFYPKEQHNQILIDIALNLTGIICQRLALKKDKTGRTAVVDLL
INTPAIQDFILKGDLMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGKTPELLYDR
VNGLNLIS

Nucleotide

• Length: 1227 bp

>NTDB_id=848519 V5G07_RS10240 WP_003697786.1 1946110..1947336(-) (pilU) [Neisseria gonorrhoeae strain WHO_Z_2024]
ATGAATACCGATAACCTGCACGACATCTTGGACGAAACGGTTCAAGTGTATTCTCAAAAAAAACAAAGCCGATCCGAAAC
CCCGGCCGAAATCGGCGCACACTTCCACCCGCTGCTCGACCGCCTGTGCGAAACCGCAGAAGCACAAAACGCGTCCGACA
TCCTTATCAGCAAAGGATTCCCGCCCTCGTTGAAAATCAACAGCGCATTAACCCCGCAGCCGCAAAAGGCTCTGACGGGC
GAGGAAACCGCCGCCATCGCCGCATCGACGATGAACGCCGAACAATCGGAAATATTCCGGCGCGACGGCGAAATCAACTA
CTCCGTCCAATCGCGCAGCGGCACGCGCTACCGCGCCAACGCCTACTACAGCCAAGGCAGCGCAGGTTTGGTTTTGCGGC
GCATCAACCACGTCATCCCGCAAATGCGGGAATTGGGCTTGCCCGAAAAACTCAAAGACCTCGCCGTCGCACCGCGCGGG
CTGCTGATTATCGTCGGGCCTACCGGTTCGGGCAAATCCACCACGATGGCGACTATGCTCGAACACCGCAACAAAACCCT
GCCCGGCCATATCGTTACCATCGAAGACCCGATTGAGTTTATCTACAAACCGCGCCGCTGCATCTTTACCCAGCGCGAAA
TCGGCGTCGACACCATAAACTGGCAGACGGCGGTACAAAACGCTATGCGCCAATCCCCAGACGTGGTCTGTATCGGCGAA
GTCCGCAGCAGGGAAAGTATGGAATACGCGATGCAGCTCGCCCAAACCGGCCACCTGTGCATTTTTACGCTCCACGCCAA
CACCGCGCCGCAGTCGCTCGAACGCATACTCAACTTCTACCCCAAAGAACAGCACAACCAAATACTGATTGACATCGCCC
TCAATCTGACCGGCATCATCTGCCAACGCCTCGCCCTCAAAAAAGACAAAACGGGCAGGACGGCGGTTGTCGACTTGCTC
ATCAACACGCCCGCCATCCAAGACTTCATCCTGAAGGGCGACCTGATGAACATCAGTAAAATCATGGAAACCGCCAAAAC
CGACGGAATGCAGACGATGGACCAAAACCTTTTCGAACTGTACCGTCACGGCATCATCAGTTACGAAGAAGCCCTGCGCC
AGTCCGTTTCCGCCAACAACCTGCGATTGCACATCCAACTGCACAAAGAAGGCAAAACGCCCGAACTCCTTTACGACAGG
GTCAACGGTCTCAACCTCATTTCCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (49-305)

3D structure

• AlphaFold DB: B4RPS5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	44.875	88.48	0.397
pilU	Pseudomonas stutzeri DSM 10701	45.225	87.255	0.395
pilU	Vibrio cholerae strain A1552	47.024	82.353	0.387