Detailed information NTG0848519

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: QTV67_RS04155
• Genome accession: NZ_CP128531
• Coordinates: 806082..807482 (+)
• Length: 466 a.a.
• NCBI ID: WP_040080755.1
• Uniprot ID: -
• Organism: Mannheimia haemolytica strain 120731
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 801082..812482

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QTV67_RS04125	-	801156..801920 (-)	765	WP_006248395.1	TSUP family transporter	-
QTV67_RS04130	proB	801920..803011 (-)	1092	WP_006251346.1	glutamate 5-kinase	-
QTV67_RS04135	-	803123..803617 (+)	495	WP_289333199.1	dihydrofolate reductase	-
QTV67_RS04140	-	803737..803967 (+)	231	WP_006248398.1	YgjV family protein	-
QTV67_RS04145	radA	804022..805401 (-)	1380	WP_040080753.1	DNA repair protein RadA	Machinery gene
QTV67_RS04150	pilA	805582..806061 (+)	480	WP_006248401.1	prepilin peptidase-dependent pilin	Machinery gene
QTV67_RS04155	pilB	806082..807482 (+)	1401	WP_040080755.1	GspE/PulE family protein	Machinery gene
QTV67_RS04160	pilC	807466..808662 (+)	1197	WP_230268904.1	type II secretion system F family protein	Machinery gene
QTV67_RS04165	-	808665..809321 (+)	657	WP_006248404.1	prepilin peptidase	-
QTV67_RS04170	coaE	809357..809992 (+)	636	WP_020831305.1	dephospho-CoA kinase	-
QTV67_RS04175	yacG	809974..810159 (+)	186	WP_006248406.1	DNA gyrase inhibitor YacG	-
QTV67_RS04180	holA	810160..811185 (-)	1026	WP_006253426.1	DNA polymerase III subunit delta	-
QTV67_RS04185	lptE	811228..811716 (-)	489	WP_006251337.1	LPS assembly lipoprotein LptE	-

Sequence

Protein

• Length: 466 a.a.
• Molecular weight: 52950.61 Da
• Isoelectric Point: 5.9587

>NTDB_id=848519 QTV67_RS04155 WP_040080755.1 806082..807482(+) (pilB) [Mannheimia haemolytica strain 120731]
MEYSVTDLNSQRFIEISAEQWQKNCNEKQILLRYLAIPLQETEEQLWLGIDDMQNLNACEIFAFIYHKQIEPVLISSDEL
KFLLNALSPEQSSRQSEIYEENTAYSLAHSEQVDRNDPIIQILENLFKFCLQHNASDIHFEPQKEKLLIRLRIDGVLHIY
KTLPQSLSSRLISRIKLLAKLDISETRLPQDGQFHFKTILAETLDFRVSTLPTHFGEKVVLRLQKNKPTQVDFLELGFNP
TQKANLRNALSQPQGLILVTGPTGSGKSITLYSALSHLNSSEKHILTAEDPIEIEIEGIIQTQVNRGINLDFSQLLRTFL
RQDPDIIMLGEIRDEESAEMALRAAQTGHLVLSTLHTNDAPSAVERLLQLGIKEYELKNSLLLVIAQRLLRRVCVKCGGA
GCEHCYQGYKGRIGVYQLLNRSAKNFEKNTACLDFKTLADSAKEKLTEGKTNQAEILRVLGNDENL

Nucleotide

• Length: 1401 bp

>NTDB_id=848519 QTV67_RS04155 WP_040080755.1 806082..807482(+) (pilB) [Mannheimia haemolytica strain 120731]
ATGGAGTATTCGGTAACGGATTTAAACAGCCAAAGGTTCATCGAGATTTCAGCAGAGCAGTGGCAGAAAAATTGCAATGA
AAAGCAGATTTTATTGCGATATTTAGCCATTCCGCTGCAAGAAACAGAAGAACAGCTTTGGCTAGGGATTGATGATATGC
AAAATCTCAATGCCTGTGAGATTTTTGCGTTTATCTACCACAAACAGATTGAGCCTGTTTTGATTTCCTCCGATGAATTG
AAGTTTTTACTCAATGCCCTTTCGCCTGAGCAGAGTAGCCGGCAGAGTGAGATTTATGAGGAGAATACGGCGTATTCATT
GGCTCATTCGGAACAAGTGGATAGAAACGATCCGATTATCCAAATTTTAGAGAATTTATTTAAGTTCTGTTTGCAACACA
ACGCCTCAGATATTCATTTTGAACCACAAAAAGAGAAATTGCTTATTCGGCTGCGAATTGATGGCGTGCTACATATTTAT
AAAACGCTTCCGCAAAGTCTGTCTAGCCGATTAATTTCTCGCATTAAATTATTGGCAAAATTAGATATTAGCGAAACCCG
CTTGCCGCAAGATGGGCAGTTTCATTTTAAAACAATTTTAGCGGAAACCTTAGATTTCCGCGTTTCAACTTTACCTACCC
ATTTCGGTGAAAAAGTTGTTCTTCGGCTTCAAAAGAATAAGCCTACGCAGGTGGATTTTTTAGAATTGGGGTTTAACCCC
ACTCAGAAAGCCAATTTGCGGAATGCTCTGAGCCAACCTCAAGGGTTGATTTTAGTCACAGGCCCGACCGGGAGCGGTAA
AAGCATCACGCTTTACAGTGCATTAAGCCATTTAAACAGCAGTGAAAAGCATATTTTAACTGCCGAAGACCCGATTGAAA
TTGAGATTGAGGGAATTATCCAAACCCAAGTGAATCGAGGGATTAATTTAGATTTCAGTCAACTGTTAAGAACTTTTCTA
CGCCAAGATCCGGACATTATTATGCTTGGCGAAATTCGTGATGAAGAGAGTGCAGAAATGGCACTACGGGCGGCACAAAC
CGGGCATTTGGTGCTTTCAACTTTGCATACCAATGATGCCCCCTCAGCGGTAGAACGGTTGCTACAGCTCGGCATAAAAG
AGTATGAACTTAAAAATTCATTGCTACTCGTGATTGCTCAACGCCTGTTACGCCGAGTGTGTGTGAAATGCGGTGGGGCA
GGCTGTGAGCATTGTTATCAAGGGTATAAAGGGCGAATTGGGGTATATCAACTCTTAAACCGATCTGCAAAAAATTTTGA
AAAAAACACCGCTTGTTTGGATTTTAAAACCCTTGCCGACAGTGCAAAAGAAAAATTGACGGAAGGAAAAACCAATCAGG
CGGAAATTTTAAGGGTGCTAGGCAATGACGAAAATCTATGA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (29-92)

• Type II/IV secretion system protein (T2SSE) (131-392)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Glaesserella parasuis strain SC1401	72.234	98.927	0.715
pilB	Haemophilus influenzae Rd KW20	57.235	99.356	0.569
pilB	Haemophilus influenzae 86-028NP	56.466	99.571	0.562
pilB	Vibrio cholerae strain A1552	40.871	100	0.423
pilB	Vibrio campbellii strain DS40M4	40.541	100	0.418
pilB	Vibrio parahaemolyticus RIMD 2210633	38.833	100	0.414
pilB	Legionella pneumophila strain ERS1305867	39.549	100	0.414
pilB	Acinetobacter baumannii D1279779	36.605	100	0.384
pilB	Acinetobacter baylyi ADP1	36.68	100	0.384