Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   QTV67_RS04150 Genome accession   NZ_CP128531
Coordinates   805582..806061 (+) Length   159 a.a.
NCBI ID   WP_006248401.1    Uniprot ID   A0A249A0S4
Organism   Mannheimia haemolytica strain 120731     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 800582..811061
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QTV67_RS04125 - 801156..801920 (-) 765 WP_006248395.1 TSUP family transporter -
  QTV67_RS04130 proB 801920..803011 (-) 1092 WP_006251346.1 glutamate 5-kinase -
  QTV67_RS04135 - 803123..803617 (+) 495 WP_289333199.1 dihydrofolate reductase -
  QTV67_RS04140 - 803737..803967 (+) 231 WP_006248398.1 YgjV family protein -
  QTV67_RS04145 radA 804022..805401 (-) 1380 WP_040080753.1 DNA repair protein RadA Machinery gene
  QTV67_RS04150 pilA 805582..806061 (+) 480 WP_006248401.1 prepilin peptidase-dependent pilin Machinery gene
  QTV67_RS04155 pilB 806082..807482 (+) 1401 WP_040080755.1 GspE/PulE family protein Machinery gene
  QTV67_RS04160 pilC 807466..808662 (+) 1197 WP_230268904.1 type II secretion system F family protein Machinery gene
  QTV67_RS04165 - 808665..809321 (+) 657 WP_006248404.1 prepilin peptidase -
  QTV67_RS04170 coaE 809357..809992 (+) 636 WP_020831305.1 dephospho-CoA kinase -
  QTV67_RS04175 yacG 809974..810159 (+) 186 WP_006248406.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 16841.15 Da        Isoelectric Point: 8.7964

>NTDB_id=848518 QTV67_RS04150 WP_006248401.1 805582..806061(+) (pilA) [Mannheimia haemolytica strain 120731]
MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVEVCIYNSGGLDNCSG
GTNGIQANKTSSNTTKYLNSIAVTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 480 bp        

>NTDB_id=848518 QTV67_RS04150 WP_006248401.1 805582..806061(+) (pilA) [Mannheimia haemolytica strain 120731]
ATGCAAAAAATTGTACATTTTAGACCGCTTGGCATTCGCCAACTGCATCGGGCATTTACCCTCATTGAGTTGATGATCGT
GATTGCGATTATTGCCATTCTTGCCACTATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTTATCGGAGT
TATTACAAGCATCTGCTTCGTATAAATCAGACGTTGAAGTCTGTATTTACAACAGTGGCGGGTTGGATAACTGCTCCGGT
GGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACAAAAGGCGT
GATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATACGATTAGTT
GGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAAACGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(48-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(16-38)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A249A0S4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

66.038

100

0.66
  pilA Haemophilus influenzae 86-028NP

56.429

88.05

0.497
  pilA Haemophilus influenzae Rd KW20

54.93

89.308

0.491