Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   V4941_RS08535 Genome accession   NZ_CP144736
Coordinates   1967281..1967778 (-) Length   165 a.a.
NCBI ID   WP_012699856.1    Uniprot ID   C1DPK4
Organism   Azotobacter vinelandii DJ     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1962281..1972778
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V4941_RS08515 (V4941_08510) - 1963291..1964070 (+) 780 WP_012699861.1 TatD family hydrolase -
  V4941_RS08520 (V4941_08515) ampE 1964231..1965073 (-) 843 WP_012699860.1 regulatory signaling modulator protein AmpE -
  V4941_RS08525 (V4941_08520) ampD 1965070..1965633 (-) 564 WP_012699859.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  V4941_RS08530 (V4941_08525) nadC 1965775..1966623 (+) 849 WP_012699858.1 carboxylating nicotinate-nucleotide diphosphorylase -
  V4941_RS08535 (V4941_08530) pilE 1967281..1967778 (-) 498 WP_012699856.1 pilin Machinery gene
  V4941_RS08540 (V4941_08535) pilB 1968307..1970007 (+) 1701 WP_012699854.1 type IV-A pilus assembly ATPase PilB Machinery gene
  V4941_RS08545 (V4941_08540) pilC 1970232..1971449 (+) 1218 WP_012699853.1 type II secretion system F family protein Machinery gene
  V4941_RS08550 (V4941_08545) pilD 1971452..1972321 (+) 870 WP_012699852.1 A24 family peptidase Machinery gene

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 16792.99 Da        Isoelectric Point: 4.5044

>NTDB_id=847618 V4941_RS08535 WP_012699856.1 1967281..1967778(-) (pilE) [Azotobacter vinelandii DJ]
MKAQAQNGFTLIELMMVVAIIGILAAVALPAYQDYTARAKVSEVVLAASSCRIAITEASQIGFAADATADGFGCGETASG
ADAPSQYVASVNTSAVGVITVVAQNISQLGTNTALEFVPYTDAGLSAAASAADFNRTTLNSVKGWKCQSAASNGIESKYL
PASCR

Nucleotide


Download         Length: 498 bp        

>NTDB_id=847618 V4941_RS08535 WP_012699856.1 1967281..1967778(-) (pilE) [Azotobacter vinelandii DJ]
ATGAAAGCTCAAGCGCAGAATGGTTTTACCCTGATCGAATTGATGATGGTGGTGGCGATCATCGGGATTTTGGCTGCGGT
AGCGTTGCCGGCTTATCAGGATTACACTGCGCGTGCCAAGGTTTCCGAAGTTGTATTGGCAGCCTCCAGTTGCCGCATCG
CGATTACCGAAGCCTCGCAGATTGGTTTCGCTGCTGATGCAACCGCCGATGGGTTCGGTTGTGGTGAGACTGCTAGTGGT
GCCGATGCTCCATCCCAGTATGTGGCATCGGTTAACACCTCTGCAGTCGGGGTTATTACTGTCGTTGCCCAAAATATTTC
CCAACTTGGCACTAATACCGCTCTGGAGTTTGTTCCTTATACCGACGCAGGCTTGTCCGCCGCTGCTAGTGCTGCGGATT
TCAATCGCACAACTCTGAACTCTGTAAAAGGATGGAAGTGTCAATCGGCCGCATCGAATGGCATAGAGTCTAAGTATCTG
CCCGCTAGCTGTCGTTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(37-165)

Prokaryotic N-terminal methylation motif (N_methyl)

(6-29)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C1DPK4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

40

100

0.424
  pilA Ralstonia pseudosolanacearum GMI1000

37.143

100

0.394
  pilA2 Legionella pneumophila strain ERS1305867

39.024

99.394

0.388
  pilA2 Legionella pneumophila str. Paris

38.889

98.182

0.382
  pilE Neisseria gonorrhoeae MS11

37.278

100

0.382
  pilA/pilA1 Eikenella corrodens VA1

36.047

100

0.376
  pilA/pilAI Pseudomonas stutzeri DSM 10701

36.527

100

0.37