Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QSG87_RS01700 Genome accession   NZ_CP127923
Coordinates   323246..324958 (-) Length   570 a.a.
NCBI ID   WP_317023907.1    Uniprot ID   -
Organism   Acinetobacter chinensis strain SAAc573     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 318246..329958
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QSG87_RS01670 (QSG87_01670) - 318362..318685 (+) 324 WP_087512620.1 pyrimidine/purine nucleoside phosphorylase -
  QSG87_RS01675 (QSG87_01675) rlmB 318817..319566 (+) 750 WP_317023903.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  QSG87_RS01680 (QSG87_01680) - 319601..320515 (+) 915 WP_317023904.1 DMT family transporter -
  QSG87_RS01685 (QSG87_01685) coaE 320517..321116 (-) 600 WP_317023905.1 dephospho-CoA kinase -
  QSG87_RS01690 (QSG87_01690) pilD 321131..321991 (-) 861 WP_087550409.1 A24 family peptidase Machinery gene
  QSG87_RS01695 (QSG87_01695) pilC 321991..323217 (-) 1227 WP_317023906.1 type II secretion system F family protein Machinery gene
  QSG87_RS01700 (QSG87_01700) pilB 323246..324958 (-) 1713 WP_317023907.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QSG87_RS01705 (QSG87_01705) tpiA 325244..326035 (+) 792 WP_317023908.1 triose-phosphate isomerase -
  QSG87_RS01710 (QSG87_01710) secG 326049..326381 (+) 333 WP_317023909.1 preprotein translocase subunit SecG -
  QSG87_RS01735 (QSG87_01735) rimP 327176..327700 (+) 525 WP_087550414.1 ribosome maturation factor RimP -
  QSG87_RS01740 (QSG87_01740) nusA 327737..329221 (+) 1485 WP_087550415.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63385.53 Da        Isoelectric Point: 4.8792

>NTDB_id=845236 QSG87_RS01700 WP_317023907.1 323246..324958(-) (pilB) [Acinetobacter chinensis strain SAAc573]
MSVLQGSPKFTGFIRRLVDEGTVSAEKMQQAVAGAKKAKQDIVPYLIEQLSLSPRTVAEKISEEFAEPIFDLAAYDTAQI
IREGIDDKLITRHRILPVFKRGHILYVATSNPTNMDAMDAIRFNSKMNIEAIIVEHNKLEKIIEQNFAEESTFDFSDDDI
DLDLDEGTSPQTTDDDDEPQADEAPIVKYINKLLIDAIRMGASDLHFEPYEKTYRVRYRVDGVLRQIATPPLQLSNRLAS
RLKVMSQMDISEKRIPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPEQKALFMEALEKP
QGMLLITGPTGSGKTVSLYTGLNILNQEDTNISTAEDPVEINLEGVNQVNVNPKVGLTFAVALKSFLRQDPDIVMVGEIR
DLETAEIAVKAAQTGHMVLSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSHCKEPVDVPRNSLLELGF
TEEDLNNSEFQVFQPVGCSECREGYKGRVGIYEVMKVTPEISKIIMEDGNAIKIAEASEKSGFNNLRRSGLVKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=845236 QSG87_RS01700 WP_317023907.1 323246..324958(-) (pilB) [Acinetobacter chinensis strain SAAc573]
ATGTCAGTATTACAGGGTTCACCTAAATTCACAGGATTTATTCGTCGCCTCGTCGATGAAGGGACAGTTTCTGCCGAAAA
AATGCAGCAGGCTGTTGCAGGAGCAAAAAAAGCCAAACAGGATATTGTTCCCTATCTGATTGAACAGCTCAGCCTTTCTC
CCAGAACTGTTGCTGAAAAAATTTCAGAAGAGTTCGCAGAACCCATTTTTGATCTTGCTGCTTATGATACTGCTCAGATT
ATCCGTGAAGGCATAGACGATAAGCTGATCACACGCCACCGTATTCTGCCTGTTTTTAAACGCGGTCACATCCTGTACGT
TGCAACCAGCAATCCAACCAATATGGATGCCATGGATGCGATCCGCTTCAACAGTAAAATGAATATTGAAGCGATTATTG
TTGAACACAATAAACTCGAAAAAATTATTGAACAGAATTTCGCTGAAGAAAGTACTTTTGATTTTTCAGATGATGATATT
GACCTGGATCTTGATGAGGGTACCTCCCCCCAGACTACAGATGACGATGATGAACCACAGGCAGATGAAGCCCCAATCGT
AAAATATATCAATAAATTACTGATTGATGCCATACGTATGGGTGCATCGGATTTACATTTTGAACCCTATGAAAAAACCT
ATCGTGTCCGCTACCGTGTAGATGGTGTACTGCGCCAGATTGCGACACCACCCTTACAGTTGTCAAACCGTCTTGCCTCC
CGACTGAAAGTCATGTCACAGATGGATATTTCTGAAAAAAGGATTCCTCAGGACGGACGTATCAAACTGAAACTGTCCAA
AACCAAAGCCATCGACTTCCGTGTCAACTCATTACCGACACTGTTTGGTGAGAAACTGGTTCTGCGTATCCTAGATCCAT
CCAGTGCCATGCTGGGCATTGATGCTTTAGGATATGAACCTGAACAGAAAGCCCTGTTTATGGAAGCTCTGGAAAAACCT
CAGGGAATGTTACTGATCACAGGTCCAACCGGTTCGGGTAAAACGGTTTCTCTTTATACCGGTCTGAATATTCTGAACCA
GGAAGACACCAACATCTCCACAGCCGAAGACCCTGTCGAAATTAACCTTGAAGGGGTTAACCAGGTGAACGTCAACCCCA
AAGTTGGTCTGACCTTCGCCGTCGCACTTAAATCATTCCTGCGTCAGGATCCTGACATTGTTATGGTCGGTGAGATCCGG
GATCTTGAAACAGCTGAAATAGCAGTCAAAGCCGCACAGACAGGACACATGGTTCTTTCCACGCTGCACACCAACAGTGC
ACCTGAAACATTGACCCGTTTGCGGAATATGGGGGTTCCATCCTTCAACATTGCAACCTCTGTCAACCTGGTGATTGCTC
AGCGACTGGCCCGTCGCTTATGTTCACACTGCAAGGAACCTGTTGATGTTCCACGTAACAGCTTACTGGAACTGGGTTTC
ACAGAAGAAGATCTGAATAATTCAGAATTCCAGGTTTTTCAGCCAGTCGGCTGTTCAGAATGCCGTGAAGGTTATAAGGG
TCGTGTCGGTATTTATGAAGTTATGAAAGTTACACCAGAAATTTCAAAGATTATTATGGAAGATGGTAATGCGATCAAAA
TTGCAGAAGCATCTGAAAAATCGGGGTTTAATAATCTTCGCCGCTCTGGATTAGTGAAAGTCATGCAGGGGGTTACTTCT
CTTCAGGAAGTAAATCGTGTGACGAGTGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.877

100

0.809
  pilB Acinetobacter baylyi ADP1

77.273

100

0.775
  pilB Legionella pneumophila strain ERS1305867

52.364

96.491

0.505
  pilF Neisseria gonorrhoeae MS11

50

98.596

0.493
  pilB Vibrio cholerae strain A1552

49.639

97.193

0.482
  pilB Vibrio campbellii strain DS40M4

50.382

91.93

0.463
  pilB Vibrio parahaemolyticus RIMD 2210633

50.287

91.754

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

34.202

100

0.368