Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QSG83_RS14140 Genome accession   NZ_CP127892
Coordinates   2714652..2716364 (+) Length   570 a.a.
NCBI ID   WP_178318340.1    Uniprot ID   -
Organism   Acinetobacter towneri strain SAAt401     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2709652..2721364
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QSG83_RS14100 (QSG83_14110) nusA 2710031..2711515 (-) 1485 WP_178318342.1 transcription termination factor NusA -
  QSG83_RS14105 (QSG83_14115) rimP 2711551..2712075 (-) 525 WP_004975871.1 ribosome maturation factor RimP -
  QSG83_RS14130 (QSG83_14140) secG 2713240..2713569 (-) 330 WP_004975868.1 preprotein translocase subunit SecG -
  QSG83_RS14135 (QSG83_14145) tpiA 2713583..2714374 (-) 792 WP_178318341.1 triose-phosphate isomerase -
  QSG83_RS14140 (QSG83_14150) pilB 2714652..2716364 (+) 1713 WP_178318340.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QSG83_RS14145 (QSG83_14155) pilC 2716395..2717621 (+) 1227 WP_004975864.1 type II secretion system F family protein Machinery gene
  QSG83_RS14150 (QSG83_14160) pilD 2717621..2718481 (+) 861 WP_178318339.1 A24 family peptidase Machinery gene
  QSG83_RS14155 (QSG83_14165) coaE 2718491..2719090 (+) 600 WP_004975860.1 dephospho-CoA kinase -
  QSG83_RS14160 (QSG83_14170) - 2719096..2719974 (-) 879 WP_394804455.1 DMT family transporter -
  QSG83_RS14165 (QSG83_14175) rlmB 2720069..2720818 (-) 750 WP_178318338.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  QSG83_RS14170 (QSG83_14180) - 2721007..2721330 (-) 324 WP_004975854.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63486.70 Da        Isoelectric Point: 4.8172

>NTDB_id=845103 QSG83_RS14140 WP_178318340.1 2714652..2716364(+) (pilB) [Acinetobacter towneri strain SAAt401]
MSVQQATPRFTGFIRRLVEDGLVSAENMQTAIKAAQKDNVDIVPYLIQNLNIAPLIIAEKISNEFGEPIFDLSVYDSALI
VREGIDEKLFSKYCILPIFKRNHLLYVATSNPTNIEAMDAIRFNSKMNIEAIVVEHDKLVKLIEQNFASESAFEFEDDDF
DLNFEEGNSEPEQAAEDEPQGDESPIVKYINKLLIDAIRMGASDLHFEPYEKTYRVRYRVDGVLRQIATPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKNKSIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPEQKELFMEALEKP
QGMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNNKVGLTFAAALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPHCKAPSDIPKQSLLEMGF
TEADLSNPEFQVYQPVGCSECREGYKGRVGVYEVMKVTPEISRIIMEDGNAIQIADASAKSGFNNLRRSGLLKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=845103 QSG83_RS14140 WP_178318340.1 2714652..2716364(+) (pilB) [Acinetobacter towneri strain SAAt401]
ATGTCAGTACAACAAGCTACACCTAGATTTACAGGTTTTATTCGACGCTTGGTCGAAGATGGGCTCGTTTCTGCTGAAAA
TATGCAAACTGCCATTAAAGCCGCACAAAAAGATAATGTTGATATTGTTCCCTATCTCATTCAAAACCTAAATATCGCGC
CTTTAATCATTGCTGAAAAAATTTCTAATGAGTTTGGCGAACCTATTTTTGATTTAAGCGTGTATGACTCTGCGCTCATC
GTGCGTGAAGGCATTGATGAAAAACTGTTCAGCAAATATTGCATATTGCCTATTTTTAAACGCAATCATCTGCTTTATGT
TGCAACCAGTAACCCGACCAATATCGAAGCAATGGATGCCATTCGTTTCAACAGTAAAATGAATATTGAAGCCATTGTGG
TCGAACACGATAAATTGGTAAAACTGATTGAACAAAACTTTGCGAGTGAGTCTGCTTTTGAATTTGAAGATGATGACTTC
GATCTCAACTTTGAAGAGGGTAACAGCGAACCAGAACAAGCTGCAGAAGATGAACCGCAGGGTGATGAATCCCCAATTGT
TAAATACATCAACAAATTACTGATTGATGCAATTCGCATGGGTGCTTCGGACTTACACTTTGAACCGTATGAAAAAACCT
ATCGAGTGCGTTACCGTGTCGATGGTGTATTACGTCAAATTGCCACCCCACCCTTACAGTTAGCTAACCGCTTGGCATCA
CGTTTAAAAGTGATGTCCCAAATGGACATTTCTGAAAAGCGTGTCCCGCAAGATGGTCGTATTAAATTAAAACTATCTAA
AAATAAATCCATCGACTTCCGTGTGAACTCGTTGCCAACATTGTTCGGTGAAAAAATCGTACTGCGTATTCTTGACCCAT
CTAGCGCGATGCTTGGGATTGACGCTTTGGGCTATGAACCAGAACAAAAAGAATTGTTCATGGAAGCCTTAGAAAAACCG
CAAGGCATGTTACTGATTACAGGTCCAACGGGTTCGGGTAAAACGGTATCGCTATATACAGGCTTAAATATCTTAAACAG
AGAAGATACCAACATTTCCACTGCCGAAGACCCTGTGGAAATTAACTTAGAAGGCATCAACCAGGTTAACGTCAACAATA
AAGTGGGCTTAACCTTCGCTGCCGCTCTGAAATCTTTCTTACGTCAAGACCCTGATATTGTCATGGTGGGTGAGATTCGT
GACCTTGAAACTGCAGAAATTGCAATTAAAGCAGCCCAAACAGGTCACATGGTTTTATCTACCCTCCACACCAACAGTGC
GCCAGAAACTTTAACGCGTTTACGCAATATGGGCGTTCCTTCCTTTAACATCGCAACTTCGGTAAACCTCGTCATTGCAC
AGCGTTTGGCACGACGCCTATGCCCGCATTGCAAAGCTCCTTCCGATATTCCAAAACAAAGCTTATTGGAAATGGGCTTT
ACTGAAGCCGATTTGAGCAATCCAGAATTTCAGGTCTATCAACCTGTAGGCTGTTCTGAATGCCGTGAAGGTTATAAAGG
ACGTGTTGGTGTTTATGAAGTGATGAAAGTCACACCAGAAATTTCTAGAATTATTATGGAGGATGGTAATGCCATCCAAA
TTGCTGATGCGTCAGCAAAATCGGGGTTTAACAATCTGCGTCGTTCGGGGTTACTTAAGGTCATGCAGGGGGTGACTTCA
TTGCAAGAAGTCAATCGTGTGACCAGTGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-151)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

78.246

100

0.782
  pilB Acinetobacter baylyi ADP1

76.316

100

0.763
  pilB Legionella pneumophila strain ERS1305867

50.523

100

0.509
  pilF Neisseria gonorrhoeae MS11

50

98.596

0.493
  pilB Vibrio cholerae strain A1552

48.587

99.298

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

48.393

98.246

0.475
  pilB Vibrio campbellii strain DS40M4

50.482

91.053

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

35.161

100

0.382
  pilF Thermus thermophilus HB27

37.435

100

0.379
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.722

98.596

0.372