Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QSH61_RS04875 Genome accession   NZ_CP127855
Coordinates   1191282..1192958 (-) Length   558 a.a.
NCBI ID   WP_206471303.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. JC3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1186282..1197958
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QSH61_RS04850 (QSH61_04850) kdsA 1186896..1187750 (+) 855 WP_206471300.1 3-deoxy-8-phosphooctulonate synthase -
  QSH61_RS04855 (QSH61_04855) - 1187836..1188741 (+) 906 WP_128725963.1 transcriptional regulator GcvA -
  QSH61_RS04860 (QSH61_04860) - 1188738..1189376 (+) 639 WP_045989837.1 alpha/beta family hydrolase -
  QSH61_RS04865 (QSH61_04865) - 1189369..1189737 (+) 369 WP_206471301.1 DUF423 domain-containing protein -
  QSH61_RS04870 (QSH61_04870) rlmM 1189751..1190836 (+) 1086 WP_206471302.1 23S rRNA (cytidine(2498)-2'-O)-methyltransferase RlmM -
  QSH61_RS04875 (QSH61_04875) pilB 1191282..1192958 (-) 1677 WP_206471303.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QSH61_RS04880 (QSH61_04880) coaE 1193068..1193697 (-) 630 WP_206471304.1 dephospho-CoA kinase -
  QSH61_RS04885 (QSH61_04885) pilD 1193707..1194585 (-) 879 WP_206471305.1 A24 family peptidase Machinery gene
  QSH61_RS04890 (QSH61_04890) pilC 1194720..1195937 (-) 1218 WP_206471306.1 type II secretion system F family protein Machinery gene
  QSH61_RS04895 (QSH61_04895) - 1195976..1196419 (-) 444 WP_206471307.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QSH61_RS04900 (QSH61_04900) nadC 1197089..1197931 (-) 843 WP_130126786.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62037.30 Da        Isoelectric Point: 5.7022

>NTDB_id=844790 QSH61_RS04875 WP_206471303.1 1191282..1192958(-) (pilB) [Pseudoalteromonas sp. JC3]
MEHHSPLLRKFITLGRVTAEQIKSRQSEANTTAELICLSSGMSSKELAEECLDLFKVPFFNLDHFNVSEIPPALVKEKLI
RKHHILPLVQKGRKLYVAASDPTDFGAFENFEFSTGLQCEVLVADYKKLEAKIDQLFDATGGLSISEEEFKEFADLEVDD
EPKQQNTNEDKDDAPIIVYINKILMDAIKKGASDLHFEPYEKKYRVRFRIDGLLHEMASPPNTLATRLAARIKVMAHLDI
AEKRKPQDGRIKLKISERKSIDFRVSTLPTMWGEKIVMRILDSSSAMLGIDVLGYEPEQKQLYLDALEQPQGMILVTGPT
GSGKTVSLYTGLNILNQPERNISTAEDPVEINLEGINQVQINTKADMTFANALRAFLRQDPDVVMVGEIRDLETAEISIK
AAQTGHLVLSTLHTNSAPETLTRLLNMGVPAYNVASSVSLIIAQRLARRLCPKCKTPETLPEEALLGQGFTAEQIKEITL
FAPKGCEHCTDGYKGRVGIYEVVKITPELSHLIMEGGNSLEIAEKAEQLGFDNLRKSGLKKAAAGVTSLTEINRVTSY

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=844790 QSH61_RS04875 WP_206471303.1 1191282..1192958(-) (pilB) [Pseudoalteromonas sp. JC3]
ATGGAACATCATTCCCCGCTACTGCGTAAATTTATTACCTTAGGGCGAGTTACTGCTGAGCAAATAAAGTCTCGCCAGTC
AGAAGCAAACACCACCGCCGAGTTGATTTGCCTAAGTTCAGGAATGAGCTCCAAAGAGCTAGCTGAAGAATGCCTAGACT
TATTCAAGGTGCCCTTTTTTAATCTTGATCACTTTAACGTTAGTGAAATTCCACCGGCGCTAGTCAAAGAAAAGCTGATC
CGTAAGCACCATATTTTACCGCTGGTACAAAAAGGTAGAAAACTCTATGTAGCGGCTTCCGATCCTACTGATTTTGGCGC
ATTTGAGAACTTTGAGTTTAGTACTGGATTACAGTGTGAAGTCTTAGTCGCCGACTACAAAAAGCTCGAAGCAAAGATTG
ATCAGCTGTTTGATGCAACGGGCGGTCTCTCGATCTCTGAAGAAGAATTTAAAGAATTTGCCGACTTAGAAGTTGATGAC
GAGCCTAAACAGCAAAACACCAACGAAGATAAAGACGATGCGCCAATCATCGTTTATATCAATAAAATCTTAATGGACGC
GATAAAAAAAGGCGCGTCGGATCTGCACTTTGAGCCCTACGAAAAAAAATACCGCGTGCGTTTTCGTATCGATGGCCTGC
TGCATGAGATGGCAAGTCCACCAAACACACTGGCTACTCGCCTCGCCGCCCGTATCAAAGTGATGGCACACTTAGACATA
GCGGAAAAACGCAAACCGCAAGATGGCCGTATTAAATTAAAGATTTCCGAGCGCAAAAGTATCGACTTTCGTGTGAGTAC
GCTGCCTACCATGTGGGGCGAGAAAATCGTAATGCGTATTCTAGATTCGTCCAGCGCCATGCTCGGCATTGACGTGCTAG
GTTATGAACCTGAGCAAAAGCAGCTTTACCTCGACGCACTAGAGCAGCCTCAAGGCATGATTTTGGTAACCGGCCCAACG
GGTTCCGGTAAAACCGTGTCACTTTACACTGGCTTGAATATTCTTAATCAGCCAGAGCGCAATATCAGCACCGCGGAAGA
CCCTGTAGAAATAAACCTTGAGGGTATTAACCAAGTACAAATCAATACCAAAGCGGATATGACTTTCGCCAATGCCTTGA
GAGCATTCTTACGTCAGGATCCGGATGTGGTGATGGTTGGTGAGATCCGTGACCTTGAAACCGCTGAAATATCAATAAAA
GCCGCCCAAACCGGTCACTTAGTATTGAGTACACTGCACACCAACTCTGCGCCTGAAACGCTCACCCGTCTATTAAACAT
GGGCGTGCCGGCGTACAACGTAGCAAGCTCGGTGAGTTTGATTATTGCCCAGCGTCTCGCAAGACGTCTTTGCCCTAAGT
GTAAAACACCTGAAACGCTGCCAGAAGAAGCCTTGCTAGGGCAAGGCTTTACTGCCGAGCAAATTAAAGAGATTACCCTT
TTTGCGCCTAAAGGCTGTGAACACTGCACCGATGGTTACAAAGGCCGTGTGGGCATTTATGAAGTGGTAAAAATCACGCC
AGAGCTTTCGCACCTGATCATGGAAGGGGGAAACTCTCTTGAGATTGCCGAAAAAGCAGAGCAGTTAGGTTTTGATAACT
TACGTAAATCTGGCCTGAAAAAAGCCGCCGCTGGAGTGACATCGCTTACAGAAATCAACCGCGTAACAAGCTATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-140)

Type II/IV secretion system protein (T2SSE)

(182-449)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.895

100

0.591
  pilB Acinetobacter baylyi ADP1

57.27

100

0.579
  pilB Vibrio cholerae strain A1552

53.455

98.566

0.527
  pilB Legionella pneumophila strain ERS1305867

54.894

93.369

0.513
  pilB Vibrio parahaemolyticus RIMD 2210633

53.977

94.624

0.511
  pilB Vibrio campbellii strain DS40M4

53.497

94.803

0.507
  pilF Neisseria gonorrhoeae MS11

47.133

100

0.471
  pilB/pilB1 Synechocystis sp. PCC 6803

36.013

100

0.401
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.429

94.086

0.371
  pilF Thermus thermophilus HB27

36.396

99.462

0.362