Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QP552_RS16360 Genome accession   NZ_CP127408
Coordinates   3542353..3544086 (-) Length   577 a.a.
NCBI ID   WP_286074072.1    Uniprot ID   -
Organism   Stenotrophomonas sp. 59     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3537353..3549086
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QP552_RS16345 sucC 3537634..3538803 (-) 1170 WP_004154430.1 ADP-forming succinate--CoA ligase subunit beta -
  QP552_RS16350 - 3539161..3540774 (+) 1614 WP_049427898.1 ATP-binding protein -
  QP552_RS16355 pilR 3540835..3542208 (+) 1374 WP_286074071.1 sigma-54 dependent transcriptional regulator Regulator
  QP552_RS16360 pilB 3542353..3544086 (-) 1734 WP_286074072.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QP552_RS16365 pilA/pilAI 3544309..3544716 (-) 408 WP_286074073.1 pilin Machinery gene
  QP552_RS16370 - 3544871..3545311 (-) 441 WP_106549568.1 pilin -
  QP552_RS16375 pilC 3545665..3546924 (+) 1260 WP_106549567.1 type II secretion system F family protein Machinery gene
  QP552_RS16380 - 3546932..3547795 (+) 864 WP_049429911.1 A24 family peptidase -
  QP552_RS16385 coaE 3547807..3548418 (+) 612 WP_286074074.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62404.34 Da        Isoelectric Point: 6.0431

>NTDB_id=844150 QP552_RS16360 WP_286074072.1 3542353..3544086(-) (pilB) [Stenotrophomonas sp. 59]
MNAVTTANLVGITGLARRLVQDGALDEATARDAMAKAAAARQPLPTWFAQNKLVNASQLAAANAVEFGMPLFDVSTFDAS
QNAMSLVSEELLRKHNVLPLFKRGGRLFVGTSNPTHSLDEIKFHTNLVVEPILVDEDQIRRTLEQWHASHDTLGDALGGD
DDGMGNLDVAAGDEDLGATGGDSGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPV
KLNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKL
FLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
IIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCGNCKRRSSLPE
HALLAEGFTQAQLDAGIELYEAVGCDECTEGYKGRTGVYQVMPMTDEIATIVLAGGNALQIAEAAQQIGVNDLRQSALKK
AAAGITSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=844150 QP552_RS16360 WP_286074072.1 3542353..3544086(-) (pilB) [Stenotrophomonas sp. 59]
ATGAACGCTGTCACCACCGCCAATCTTGTCGGCATCACCGGCCTGGCCCGTCGCCTGGTGCAGGACGGGGCCCTGGACGA
GGCCACCGCCCGCGACGCCATGGCCAAGGCCGCGGCCGCCCGCCAGCCACTGCCAACGTGGTTCGCGCAGAACAAGCTGG
TCAATGCCTCGCAGCTGGCCGCCGCCAACGCGGTCGAGTTCGGCATGCCGCTGTTCGACGTGTCCACCTTCGACGCCAGC
CAGAACGCGATGAGCCTGGTCAGCGAGGAGCTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAAGCGCGGTGGCAGGCT
GTTCGTCGGCACCAGCAACCCGACCCACTCGCTGGACGAGATCAAGTTCCACACCAACCTGGTGGTCGAGCCGATCCTGG
TCGACGAGGACCAGATCCGCCGCACGCTGGAACAGTGGCACGCCAGCCATGACACCCTCGGCGACGCCCTGGGTGGCGAT
GACGATGGCATGGGCAACCTGGACGTGGCGGCCGGCGACGAGGATCTCGGCGCTACCGGCGGCGATTCCGGCATCGACGC
CAAGGGCGACGACACCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTCGATGCGATCCGCAAGGGCGCCTCGGACATCC
ACTTCGAGCCCTACGAAGATGATTACCGCGTGCGCCTGCGCATCGATGGCCTGCTGAAGATGGTCGCGCGCGCACCGGTG
AAGTTGAACCAGCGCATCGCCGCCCGCCTGAAGGTGATGGCACAGCTGGACATCGCCGAGAAGCGCGTCCCGCAGGACGG
CCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACGCTGCCGACCCTGTTCGGCGAGAAGG
TGGTGCTGCGTATCCTTGATGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGTTACGAGCCGGACCAGCAGAAGCTG
TTCCTGGATGCCATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACCGTGTCGCTGTA
TACCGCGCTGGGCATCCTCAACGACGAGACGCGCAACATCTCCACCGCCGAAGACCCGGTGGAAATCCGCCTGCCCGGCG
TCAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCAGCGCTGCGCTCGTTCCTGCGACAGGACCCGGAC
ATCATCATGGTCGGCGAAATCCGCGACCTGGAAACGGCGGAGATCGCGATCAAGGCCGCGCAGACCGGCCACATGGTGCT
GTCCACGCTGCACACCAACGATGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCGCCGTACAACATCACCA
GCTCGGTGACGCTGGTGATCGCCCAGCGCCTGGCGCGCCGCCTGTGCGGCAACTGCAAGCGCCGGTCCAGCCTGCCCGAA
CATGCACTGCTGGCCGAAGGCTTCACCCAGGCGCAGCTGGATGCGGGCATCGAGCTGTACGAGGCCGTGGGCTGCGATGA
ATGCACCGAAGGCTACAAGGGCCGGACCGGCGTCTACCAGGTCATGCCGATGACCGATGAGATCGCCACCATCGTGCTGG
CCGGTGGCAATGCGCTGCAGATTGCCGAGGCGGCGCAGCAGATCGGCGTGAATGACCTGCGCCAGTCGGCGTTGAAGAAG
GCCGCGGCGGGCATCACCAGCCTGGCCGAGATCAACCGCGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.701

100

0.555
  pilB Acinetobacter baumannii D1279779

54.828

100

0.551
  pilB Legionella pneumophila strain ERS1305867

52.105

98.787

0.515
  pilF Neisseria gonorrhoeae MS11

48.596

98.787

0.48
  pilB Vibrio cholerae strain A1552

48.759

97.747

0.477
  pilB Vibrio parahaemolyticus RIMD 2210633

45.76

98.094

0.449
  pilB Vibrio campbellii strain DS40M4

45.343

98.614

0.447