Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   V1232_RS06280 Genome accession   NZ_CP143357
Coordinates   1369002..1369541 (+) Length   179 a.a.
NCBI ID   WP_031285592.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 2023CK-01621     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1364002..1374541
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V1232_RS06255 (V1232_06255) yacG 1364169..1364369 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  V1232_RS06260 (V1232_06260) coaE 1364366..1364977 (-) 612 WP_003094654.1 dephospho-CoA kinase -
  V1232_RS06265 (V1232_06265) pilD 1364974..1365846 (-) 873 WP_003116251.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  V1232_RS06270 (V1232_06270) pilC 1365849..1367066 (-) 1218 WP_023435364.1 type II secretion system F family protein Machinery gene
  V1232_RS06275 (V1232_06275) pilB 1367070..1368773 (-) 1704 WP_003116249.1 type IV-A pilus assembly ATPase PilB Machinery gene
  V1232_RS06280 (V1232_06280) pilE 1369002..1369541 (+) 540 WP_031285592.1 pilin Machinery gene
  V1232_RS06285 (V1232_06285) - 1369605..1370303 (+) 699 WP_016254081.1 hypothetical protein -
  V1232_RS06295 (V1232_06295) - 1370546..1370788 (-) 243 WP_023082027.1 hypothetical protein -
  V1232_RS06300 (V1232_06300) - 1371817..1372076 (-) 260 Protein_1235 EscU/YscU/HrcU family type III secretion system export apparatus switch protein -
  V1232_RS06305 (V1232_06305) fliR 1372079..1372861 (-) 783 Protein_1236 flagellar biosynthetic protein FliR -
  V1232_RS06310 (V1232_06310) fliQ 1372861..1373130 (-) 270 WP_023435365.1 flagellar biosynthesis protein FliQ -
  V1232_RS06315 (V1232_06315) fliP 1373175..1373930 (-) 756 WP_031285507.1 flagellar type III secretion system pore protein FliP -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 18084.43 Da        Isoelectric Point: 4.7718

>NTDB_id=843589 V1232_RS06280 WP_031285592.1 1369002..1369541(+) (pilE) [Pseudomonas aeruginosa strain 2023CK-01621]
MKAQKGFTLIELMIVVAIIGILAAVALPAYQDYTIRARVTEGVGLAASAKTLIGDSSATAGELAASARVWNAQAGDAGAT
SKYVTSVRIAEATGEITVTFNATNVGNIPADSTLVFTPYVQNAAGAPTQLGASYAAGVTGSIDWGCASESNAVSSGPDRN
MPALTAGTLPARFAPSECR

Nucleotide


Download         Length: 540 bp        

>NTDB_id=843589 V1232_RS06280 WP_031285592.1 1369002..1369541(+) (pilE) [Pseudomonas aeruginosa strain 2023CK-01621]
ATGAAAGCTCAGAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATTCTTGCTGCCGTCGCTTT
GCCGGCATATCAGGATTACACCATTCGTGCTCGCGTGACAGAGGGGGTTGGTCTGGCTGCCAGCGCCAAGACGCTTATTG
GCGATAGCTCTGCCACTGCCGGTGAGCTAGCCGCTTCGGCAAGGGTCTGGAATGCTCAAGCCGGTGACGCCGGTGCTACC
AGTAAGTATGTGACCTCTGTACGAATTGCAGAGGCGACTGGTGAAATCACTGTTACTTTCAATGCCACAAACGTGGGTAA
TATTCCGGCTGACTCTACCCTGGTATTTACTCCCTATGTGCAGAATGCTGCCGGTGCCCCGACTCAATTGGGTGCCAGTT
ATGCTGCCGGTGTGACTGGCTCTATTGACTGGGGTTGTGCATCGGAATCCAATGCGGTTTCCAGTGGTCCCGACCGCAAT
ATGCCTGCCCTGACTGCAGGTACCCTGCCGGCTCGTTTCGCTCCGAGCGAATGCCGCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(36-179)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

42.64

100

0.469
  pilA Ralstonia pseudosolanacearum GMI1000

43.243

100

0.447
  pilA2 Legionella pneumophila str. Paris

41.477

98.324

0.408
  pilA2 Legionella pneumophila strain ERS1305867

40.909

98.324

0.402
  comP Acinetobacter baylyi ADP1

38.333

100

0.385
  pilE Neisseria gonorrhoeae strain FA1090

35.714

100

0.363