Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   V1232_RS06265 Genome accession   NZ_CP143357
Coordinates   1364974..1365846 (-) Length   290 a.a.
NCBI ID   WP_003116251.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 2023CK-01621     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1359974..1370846
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V1232_RS06220 (V1232_06220) - 1360835..1361005 (-) 171 WP_003094672.1 DUF3094 family protein -
  V1232_RS06225 (V1232_06225) - 1361031..1361480 (-) 450 WP_003094670.1 MOSC domain-containing protein -
  V1232_RS06230 (V1232_06230) - 1361477..1362106 (-) 630 WP_003094668.1 DUF1780 domain-containing protein -
  V1232_RS06235 (V1232_06235) - 1362239..1362664 (+) 426 WP_023435363.1 GNAT family N-acetyltransferase -
  V1232_RS06240 (V1232_06240) - 1362661..1363131 (+) 471 WP_003094664.1 hypothetical protein -
  V1232_RS06245 (V1232_06245) - 1363128..1363817 (+) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  V1232_RS06250 (V1232_06250) - 1363923..1364132 (+) 210 WP_003094660.1 hypothetical protein -
  V1232_RS06255 (V1232_06255) yacG 1364169..1364369 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  V1232_RS06260 (V1232_06260) coaE 1364366..1364977 (-) 612 WP_003094654.1 dephospho-CoA kinase -
  V1232_RS06265 (V1232_06265) pilD 1364974..1365846 (-) 873 WP_003116251.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  V1232_RS06270 (V1232_06270) pilC 1365849..1367066 (-) 1218 WP_023435364.1 type II secretion system F family protein Machinery gene
  V1232_RS06275 (V1232_06275) pilB 1367070..1368773 (-) 1704 WP_003116249.1 type IV-A pilus assembly ATPase PilB Machinery gene
  V1232_RS06280 (V1232_06280) pilE 1369002..1369541 (+) 540 WP_031285592.1 pilin Machinery gene
  V1232_RS06285 (V1232_06285) - 1369605..1370303 (+) 699 WP_016254081.1 hypothetical protein -
  V1232_RS06295 (V1232_06295) - 1370546..1370788 (-) 243 WP_023082027.1 hypothetical protein -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 31871.89 Da        Isoelectric Point: 7.3454

>NTDB_id=843586 V1232_RS06265 WP_003116251.1 1364974..1365846(-) (pilD) [Pseudomonas aeruginosa strain 2023CK-01621]
MELLDYLASHPLAFVLCAILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide


Download         Length: 873 bp        

>NTDB_id=843586 V1232_RS06265 WP_003116251.1 1364974..1365846(-) (pilD) [Pseudomonas aeruginosa strain 2023CK-01621]
ATGGAACTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCCATCCTCCTTGGCCTGCTGGTTGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAGGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTATGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGCCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGTCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(137-245)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

55.017

99.655

0.548
  pilD Vibrio campbellii strain DS40M4

53.497

98.621

0.528
  pilD Acinetobacter nosocomialis M2

50.352

97.931

0.493
  pilD Acinetobacter baumannii D1279779

50.352

97.931

0.493
  pilD Neisseria gonorrhoeae MS11

49.281

95.862

0.472