Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QO259_RS06345 Genome accession   NZ_CP127244
Coordinates   1391410..1393137 (-) Length   575 a.a.
NCBI ID   WP_285952214.1    Uniprot ID   A0A9Y2DCU2
Organism   Salinicola sp. JS01     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1386410..1398137
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QO259_RS06320 (QO259_06320) - 1386958..1387911 (+) 954 WP_285952210.1 Nudix family hydrolase -
  QO259_RS06325 (QO259_06325) yacG 1388078..1388329 (-) 252 WP_051895981.1 DNA gyrase inhibitor YacG -
  QO259_RS06330 (QO259_06330) coaE 1388326..1388991 (-) 666 WP_285952211.1 dephospho-CoA kinase -
  QO259_RS06335 (QO259_06335) - 1388988..1389848 (-) 861 WP_285952212.1 A24 family peptidase -
  QO259_RS06340 (QO259_06340) pilC 1390068..1391294 (-) 1227 WP_285952213.1 type II secretion system F family protein Machinery gene
  QO259_RS06345 (QO259_06345) pilB 1391410..1393137 (-) 1728 WP_285952214.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QO259_RS06355 (QO259_06355) - 1393379..1393900 (+) 522 WP_353057319.1 pilin -
  QO259_RS06360 (QO259_06360) - 1393980..1394264 (-) 285 WP_035473735.1 hypothetical protein -
  QO259_RS06365 (QO259_06365) - 1394466..1395965 (+) 1500 WP_285952743.1 TrkH family potassium uptake protein -
  QO259_RS06370 (QO259_06370) - 1396154..1397356 (-) 1203 WP_285952215.1 1-acyl-sn-glycerol-3-phosphate acyltransferase -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63367.54 Da        Isoelectric Point: 5.4843

>NTDB_id=843033 QO259_RS06345 WP_285952214.1 1391410..1393137(-) (pilB) [Salinicola sp. JS01]
MPTTTSPAIRSGIARRLIDRGLIEAGACERAQQAARDAEQTLLEFLVRQGRVDPRQAGEAAAREYGLPFLDLQHVVWERL
PIIEPIAEKLIGRFQVLPLARDERRLTVAIAFPATLAKLDELQFTTGLAIDGVLVPFDQLERALARRLQHQDTSGMLEAL
DEASDAVTLDTSEIDLEEEAAALDAGGDNDAPIVRFVNRVLRDAIQRGASDVHFEPYESSFRVRFRIDGMLLEVTRPPLN
LRTRIAARLKVMARLDISERRLPQDGAMKLRLEDQRAIDFRVNSLPTVFGEKIVLRLLDARATGWGIDALGFSPPQRELF
ESALARPQGMILVTGPTGSGKTVTLYTGLNRLNTEERNISTAEDPVELKIPGINQVNVQSRIGLDFASALRAFLRQDPDV
VMVGEIRDLETAEIAVKAAQTGHLVLSTLHTNSAAETLTRLRNMGLASFNIASSVSLIIAQRLARRLCPHCRVPSQVPDE
VLLGEGVSRERLANATLYRPGGCDRCTLGYKGRQGIYEVVPIDDAMSQLIMRDGHALELAQLARERGYPGLRQSAIELAL
SGVTSLEEVNRVTLD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=843033 QO259_RS06345 WP_285952214.1 1391410..1393137(-) (pilB) [Salinicola sp. JS01]
GTGCCGACCACCACCTCCCCTGCCATCCGTAGCGGTATCGCCCGCCGCCTTATCGATCGCGGCCTGATCGAGGCTGGCGC
GTGCGAGCGTGCGCAGCAGGCGGCGAGAGACGCCGAGCAGACGCTGCTCGAGTTCCTGGTGCGCCAGGGCCGGGTCGACC
CGCGTCAGGCCGGCGAGGCGGCGGCGCGCGAGTACGGGCTGCCGTTTCTCGACCTGCAGCACGTGGTGTGGGAACGCCTG
CCGATCATCGAACCGATTGCGGAAAAGCTGATCGGCCGCTTCCAGGTACTGCCGCTGGCGCGTGACGAACGCCGGCTGAC
GGTGGCGATCGCTTTTCCCGCCACCCTGGCCAAGCTCGATGAACTTCAGTTCACCACTGGCCTTGCGATCGACGGCGTGC
TGGTGCCGTTCGACCAGCTCGAGCGCGCCCTGGCCCGCCGCCTGCAGCACCAGGACACCTCGGGCATGCTCGAAGCGCTG
GATGAAGCCAGCGATGCGGTGACGCTGGATACCAGCGAGATCGACCTGGAAGAGGAAGCCGCGGCCCTGGATGCCGGTGG
CGACAACGACGCGCCGATCGTGCGCTTCGTCAACCGCGTGCTGCGCGATGCGATCCAGCGCGGGGCCTCGGACGTGCACT
TCGAGCCCTACGAGTCGAGCTTTCGGGTGCGCTTTCGCATCGACGGCATGCTGCTGGAGGTGACCCGCCCGCCGCTCAAC
CTGCGCACCCGCATCGCCGCCCGCCTCAAGGTCATGGCACGCCTGGACATCTCCGAGCGCCGCCTGCCCCAGGACGGCGC
AATGAAACTGCGTCTGGAGGATCAGCGCGCCATCGACTTTCGCGTCAACAGCCTGCCCACGGTATTCGGCGAGAAGATCG
TGCTACGCCTGCTCGATGCCCGCGCCACTGGATGGGGCATCGATGCGCTCGGTTTTTCACCGCCCCAGCGCGAGCTGTTC
GAAAGCGCCCTGGCGCGCCCCCAGGGGATGATTCTGGTGACCGGCCCCACCGGCAGCGGCAAGACGGTGACCCTCTACAC
CGGGCTCAATCGGCTCAATACCGAGGAGCGCAACATCTCCACCGCCGAGGACCCGGTGGAACTCAAGATACCGGGCATCA
ACCAGGTCAACGTGCAGTCACGTATCGGACTCGATTTCGCCAGCGCCCTGCGCGCCTTTCTGCGCCAGGACCCGGACGTG
GTGATGGTCGGTGAGATTCGCGATCTGGAAACCGCAGAGATCGCGGTCAAGGCGGCCCAGACCGGTCACCTGGTGCTCTC
CACCCTGCACACCAACTCTGCCGCCGAGACGCTGACGCGACTGCGCAACATGGGGCTGGCAAGCTTCAATATCGCCAGTT
CGGTGTCGCTGATCATCGCGCAGCGCCTGGCGCGCCGGCTCTGCCCCCACTGCCGGGTGCCGTCTCAAGTGCCCGACGAG
GTGCTGCTCGGCGAGGGCGTATCACGCGAGCGCCTGGCCAACGCCACGCTCTATCGCCCGGGCGGCTGCGACCGCTGCAC
GCTGGGCTACAAGGGTCGCCAGGGGATCTATGAAGTGGTGCCTATCGATGACGCCATGAGCCAATTGATCATGCGTGACG
GCCACGCCCTGGAACTGGCGCAGCTGGCGCGTGAGCGCGGCTATCCCGGCCTGCGCCAGAGCGCCATCGAGCTGGCGCTC
AGCGGCGTGACCAGCCTGGAGGAAGTCAATCGGGTGACCCTCGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-466)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

51.031

100

0.517
  pilB Acinetobacter baumannii D1279779

51.049

99.478

0.508
  pilB Legionella pneumophila strain ERS1305867

50.617

98.609

0.499
  pilB Vibrio parahaemolyticus RIMD 2210633

46.632

100

0.47
  pilB Vibrio campbellii strain DS40M4

50.467

93.043

0.47
  pilB Vibrio cholerae strain A1552

50.584

89.391

0.452
  pilF Neisseria gonorrhoeae MS11

45.614

99.13

0.452
  pilF Thermus thermophilus HB27

38.652

98.087

0.379
  pilB/pilB1 Synechocystis sp. PCC 6803

39.411

94.435

0.372
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40

93.043

0.372