Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   VYI58_RS01485 Genome accession   NZ_CP142953
Coordinates   306547..307311 (-) Length   254 a.a.
NCBI ID   WP_001136234.1    Uniprot ID   -
Organism   Escherichia coli strain EC190759     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 301547..312311
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VYI58_RS01455 (VYI58_01455) - 302181..303539 (-) 1359 WP_001545659.1 PTS galactitol transporter subunit IIC -
  VYI58_RS01460 (VYI58_01460) - 303616..303897 (-) 282 WP_000084021.1 PTS sugar transporter subunit IIB -
  VYI58_RS01465 (VYI58_01465) - 303894..304367 (-) 474 WP_001161648.1 PTS sugar transporter subunit IIA -
  VYI58_RS01470 (VYI58_01470) - 304392..305138 (-) 747 WP_001296489.1 GntR family transcriptional regulator -
  VYI58_RS01475 (VYI58_01475) nikR 305337..305738 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  VYI58_RS01480 (VYI58_01480) nikE 305744..306550 (-) 807 WP_000173682.1 nickel import ATP-binding protein NikE -
  VYI58_RS01485 (VYI58_01485) amiE 306547..307311 (-) 765 WP_001136234.1 nickel import ATP-binding protein NikD Regulator
  VYI58_RS01490 (VYI58_01490) nikC 307311..308144 (-) 834 WP_001008957.1 nickel ABC transporter permease subunit NikC -
  VYI58_RS01495 (VYI58_01495) nikB 308141..309085 (-) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  VYI58_RS01500 (VYI58_01500) nikA 309085..310659 (-) 1575 WP_000493122.1 nickel ABC transporter substrate-binding protein -
  VYI58_RS01505 (VYI58_01505) acpT 310770..311357 (-) 588 WP_000285789.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26811.42 Da        Isoelectric Point: 6.9014

>NTDB_id=843033 VYI58_RS01485 WP_001136234.1 306547..307311(-) (amiE) [Escherichia coli strain EC190759]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGNVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=843033 VYI58_RS01485 WP_001136234.1 306547..307311(-) (amiE) [Escherichia coli strain EC190759]
ATGCCACAACAGATTGAACTCCGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTGCA
ACGTGGGCGTGTGCTGGCGTTAGTCGGCGGTAGTGGCAGCGGGAAGTCGCTGACCTGCGCCGCGACGCTGGGCATTCTGC
CCGCAGGCGTTCGCCAGACGGCGGGGGAAATTTTGGCCGATGGAAAACCGGTTTCTCCCTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGTGCCTTTAATCCGCTGCACACCATGCACACCCACGCGCGTGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTCTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGCGAATCACCG
TTTATCATCGCCGATGAACCAACCACCGATCTCGACGTGGTAGCACAAGCACGCATCCTCGATCTACTGGAAAGCATTAT
GCAAAAACAAGCACCGGGAATGCTGCTGGTTACCCATGATATGGGCGTAGTAGCGCGTCTGGCAGACGATGTGGCGGTAA
TGTCTCACGGTAAAATTGTCGAACAGGGCAATGTAGAAACGCTGTTTAACGCCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398