Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   VYI56_RS20165 Genome accession   NZ_CP142915
Coordinates   4058203..4059588 (+) Length   461 a.a.
NCBI ID   WP_032202336.1    Uniprot ID   -
Organism   Escherichia coli strain B19374     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4053203..4064588
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VYI56_RS20140 (VYI56_20140) aroP 4053751..4055124 (+) 1374 WP_000969912.1 aromatic amino acid transporter AroP -
  VYI56_RS20145 (VYI56_20145) ampE 4055167..4056021 (-) 855 WP_000172014.1 beta-lactamase regulator AmpE -
  VYI56_RS20150 (VYI56_20150) ampD 4056018..4056569 (-) 552 WP_000923722.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VYI56_RS20155 (VYI56_20155) nadC 4056657..4057550 (+) 894 WP_001135176.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VYI56_RS20160 (VYI56_20160) pilA 4057753..4058193 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  VYI56_RS20165 (VYI56_20165) pilB 4058203..4059588 (+) 1386 WP_032202336.1 type II secretion system protein GspE Machinery gene
  VYI56_RS20170 (VYI56_20170) hofC 4059578..4060780 (+) 1203 WP_000157266.1 protein transport protein HofC -
  VYI56_RS20175 (VYI56_20175) guaC 4060815..4061858 (-) 1044 WP_001217337.1 GMP reductase -
  VYI56_RS20180 (VYI56_20180) - 4062014..4062058 (-) 45 WP_120795372.1 protein YacM -
  VYI56_RS20185 (VYI56_20185) coaE 4062083..4062703 (+) 621 WP_001269527.1 dephospho-CoA kinase -
  VYI56_RS20190 (VYI56_20190) zapD 4062703..4063446 (+) 744 WP_001194721.1 cell division protein ZapD -
  VYI56_RS20195 (VYI56_20195) yacG 4063456..4063653 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  VYI56_RS20200 (VYI56_20200) mutT 4063869..4064258 (-) 390 WP_000736019.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50641.37 Da        Isoelectric Point: 6.6256

>NTDB_id=842495 VYI56_RS20165 WP_032202336.1 4058203..4059588(+) (pilB) [Escherichia coli strain B19374]
MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEYRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVGQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQKLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=842495 VYI56_RS20165 WP_032202336.1 4058203..4059588(+) (pilB) [Escherichia coli strain B19374]
ATGAATATTCCACAGCTCACTGCCCTGTGTCTGCGTTATCATGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTAACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAATATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGGGTCAGGCACTGGATGTCAACACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACCGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAAAGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCAGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACAGCAGAGATCGCTATTAAAGCGGCGCAAACTGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGGGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCACATTGTCGCCGGCAGCAAGGGGAGCCCATCCACATTCCAGACAATGTATGGC
CATCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTGCCCATAACGCCGGTCATTCGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCACGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTAGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TACTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)

Type II/IV secretion system protein (T2SSE)

(88-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

42.443

100

0.445
  pilB Glaesserella parasuis strain SC1401

41.253

100

0.414
  pilB Legionella pneumophila strain ERS1305867

49.738

82.863

0.412
  pilB Acinetobacter baylyi ADP1

40.171

100

0.408
  pilB Vibrio cholerae strain A1552

46.667

84.599

0.395
  pilB Vibrio parahaemolyticus RIMD 2210633

46.154

84.599

0.39
  pilB Haemophilus influenzae 86-028NP

45.22

83.948

0.38
  pilB Acinetobacter baumannii D1279779

43.655

85.466

0.373
  pilB Haemophilus influenzae Rd KW20

44.444

83.948

0.373
  pilF Neisseria gonorrhoeae MS11

44.416

83.514

0.371