Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   VYI56_RS20160 Genome accession   NZ_CP142915
Coordinates   4057753..4058193 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain B19374     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4052753..4063193
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VYI56_RS20140 (VYI56_20140) aroP 4053751..4055124 (+) 1374 WP_000969912.1 aromatic amino acid transporter AroP -
  VYI56_RS20145 (VYI56_20145) ampE 4055167..4056021 (-) 855 WP_000172014.1 beta-lactamase regulator AmpE -
  VYI56_RS20150 (VYI56_20150) ampD 4056018..4056569 (-) 552 WP_000923722.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VYI56_RS20155 (VYI56_20155) nadC 4056657..4057550 (+) 894 WP_001135176.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VYI56_RS20160 (VYI56_20160) pilA 4057753..4058193 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  VYI56_RS20165 (VYI56_20165) pilB 4058203..4059588 (+) 1386 WP_032202336.1 type II secretion system protein GspE Machinery gene
  VYI56_RS20170 (VYI56_20170) hofC 4059578..4060780 (+) 1203 WP_000157266.1 protein transport protein HofC -
  VYI56_RS20175 (VYI56_20175) guaC 4060815..4061858 (-) 1044 WP_001217337.1 GMP reductase -
  VYI56_RS20180 (VYI56_20180) - 4062014..4062058 (-) 45 WP_120795372.1 protein YacM -
  VYI56_RS20185 (VYI56_20185) coaE 4062083..4062703 (+) 621 WP_001269527.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=842494 VYI56_RS20160 WP_000360904.1 4057753..4058193(+) (pilA) [Escherichia coli strain B19374]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=842494 VYI56_RS20160 WP_000360904.1 4057753..4058193(+) (pilA) [Escherichia coli strain B19374]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363