Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   VJY30_RS19775 Genome accession   NZ_CP142035
Coordinates   4053766..4055151 (+) Length   461 a.a.
NCBI ID   WP_001025211.1    Uniprot ID   -
Organism   Escherichia coli strain HS4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4048766..4060151
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VJY30_RS19750 (VJY30_19750) aroP 4049314..4050687 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  VJY30_RS19755 (VJY30_19755) ampE 4050730..4051584 (-) 855 WP_000172020.1 beta-lactamase regulator AmpE -
  VJY30_RS19760 (VJY30_19760) ampD 4051581..4052132 (-) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VJY30_RS19765 (VJY30_19765) nadC 4052220..4053113 (+) 894 WP_001135167.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VJY30_RS19770 (VJY30_19770) pilA 4053316..4053756 (+) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  VJY30_RS19775 (VJY30_19775) pilB 4053766..4055151 (+) 1386 WP_001025211.1 type II secretion system protein GspE Machinery gene
  VJY30_RS19780 (VJY30_19780) hofC 4055141..4056343 (+) 1203 WP_000157219.1 protein transport protein HofC -
  VJY30_RS19785 (VJY30_19785) guaC 4056378..4057421 (-) 1044 WP_001217333.1 GMP reductase -
  VJY30_RS19790 (VJY30_19790) - 4057577..4057621 (-) 45 WP_120795372.1 protein YacM -
  VJY30_RS19795 (VJY30_19795) coaE 4057646..4058266 (+) 621 WP_001269520.1 dephospho-CoA kinase -
  VJY30_RS19800 (VJY30_19800) zapD 4058266..4059009 (+) 744 WP_001194731.1 cell division protein ZapD -
  VJY30_RS19805 (VJY30_19805) yacG 4059019..4059216 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  VJY30_RS19810 (VJY30_19810) mutT 4059305..4059703 (-) 399 WP_000736040.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50597.31 Da        Isoelectric Point: 6.6254

>NTDB_id=839486 VJY30_RS19775 WP_001025211.1 4053766..4055151(+) (pilB) [Escherichia coli strain HS4]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPVVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHSRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPIIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=839486 VJY30_RS19775 WP_001025211.1 4053766..4055151(+) (pilB) [Escherichia coli strain HS4]
ATGAATATTCCACAGCTCACCGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTAGAACGTTACAGTCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGTCGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAATCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGCCAGGCACTGGATGTTAACACGCTGGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCATTGCAACAAC
CACAGGGATTGGTGCTGGTAACTGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATTC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACGGCAGAAATTGCCATTAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGAGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCGCATTGTCGCCGGCAGCAAGGGGAGCCCATCCATATTCCAGACAATGTATGGC
CGTCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTACCCATAACACCGATCATACGCCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACACACGCCCGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TATTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)

Type II/IV secretion system protein (T2SSE)

(88-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

50

83.297

0.416
  pilB Glaesserella parasuis strain SC1401

41.253

100

0.414
  pilB Acinetobacter baylyi ADP1

40.171

100

0.408
  pilB Vibrio campbellii strain DS40M4

48.196

84.165

0.406
  pilB Vibrio cholerae strain A1552

46.41

84.599

0.393
  pilB Vibrio parahaemolyticus RIMD 2210633

46.154

84.599

0.39
  pilB Haemophilus influenzae 86-028NP

44.961

83.948

0.377
  pilB Acinetobacter baumannii D1279779

43.939

85.9

0.377
  pilF Neisseria gonorrhoeae MS11

44.675

83.514

0.373
  pilB Haemophilus influenzae Rd KW20

44.186

83.948

0.371