Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   ALDI51_RS19485 Genome accession   NZ_AP024172
Coordinates   4121271..4121795 (-) Length   174 a.a.
NCBI ID   WP_201396210.1    Uniprot ID   -
Organism   Alicycliphilus denitrificans strain I51     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4116271..4126795
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ALDI51_RS19465 (ALDI51_38500) - 4117257..4118204 (-) 948 WP_236598406.1 Bug family tripartite tricarboxylate transporter substrate binding protein -
  ALDI51_RS19470 (ALDI51_38510) - 4118395..4118814 (+) 420 WP_201396207.1 hypothetical protein -
  ALDI51_RS19475 (ALDI51_38520) miaB 4118881..4120227 (+) 1347 WP_201396208.1 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB -
  ALDI51_RS19480 (ALDI51_38530) - 4120419..4121180 (-) 762 WP_201396209.1 hypothetical protein -
  ALDI51_RS19485 (ALDI51_38540) pilE 4121271..4121795 (-) 525 WP_201396210.1 pilin Machinery gene
  ALDI51_RS19490 (ALDI51_38550) - 4122019..4122465 (+) 447 WP_201396211.1 hypothetical protein -
  ALDI51_RS19495 (ALDI51_38570) vapB 4122757..4122990 (+) 234 WP_201396212.1 type II toxin-antitoxin system VapB family antitoxin -
  ALDI51_RS19500 (ALDI51_38580) vapC 4122990..4123391 (+) 402 WP_236598242.1 type II toxin-antitoxin system tRNA(fMet)-specific endonuclease VapC -
  ALDI51_RS19505 (ALDI51_38590) ffh 4123465..4124844 (-) 1380 WP_013722921.1 signal recognition particle protein -
  ALDI51_RS19510 (ALDI51_38600) - 4124934..4125731 (+) 798 WP_094438286.1 inner membrane protein YpjD -
  ALDI51_RS19515 (ALDI51_38610) - 4125728..4125955 (+) 228 WP_201396214.1 PP0621 family protein -

Sequence

Protein


Download         Length: 174 a.a.        Molecular weight: 18012.64 Da        Isoelectric Point: 8.9716

>NTDB_id=83833 ALDI51_RS19485 WP_201396210.1 4121271..4121795(-) (pilE) [Alicycliphilus denitrificans strain I51]
MKANLQKGFTLIELMIVVAIIGILAAVALPAYQDYTVRSKVSEVIIAGTSPKSLISEAFQSDGLVGAQNAAKEYNLRAAT
EKSSKYVEGIAIDEATAVITVTTKNNASAGLPTDATNKTIVFSPNVNKLALATGVTGAIDWACTSATDTTATTRGLGSKA
KGTLPAKYAPSECR

Nucleotide


Download         Length: 525 bp        

>NTDB_id=83833 ALDI51_RS19485 WP_201396210.1 4121271..4121795(-) (pilE) [Alicycliphilus denitrificans strain I51]
ATGAAGGCGAATCTACAAAAGGGTTTCACCCTGATCGAACTGATGATCGTGGTGGCGATCATTGGTATTTTGGCGGCCGT
GGCACTTCCAGCCTACCAAGACTATACGGTTCGTTCTAAGGTGTCTGAAGTAATCATCGCTGGCACTTCACCAAAGAGCC
TGATCAGCGAGGCATTCCAGAGTGATGGTCTGGTAGGGGCTCAGAATGCCGCAAAAGAATACAACTTGCGCGCTGCGACT
GAAAAGTCATCTAAATATGTTGAGGGCATTGCAATCGATGAAGCCACTGCAGTTATTACTGTCACAACTAAAAACAACGC
TTCTGCAGGACTTCCAACGGATGCGACAAATAAAACAATCGTATTTTCGCCGAATGTTAATAAGCTGGCGCTTGCTACCG
GCGTGACAGGTGCAATTGATTGGGCATGTACCTCGGCGACGGATACGACCGCAACGACTCGTGGTTTGGGTAGCAAGGCG
AAGGGTACTTTGCCCGCGAAGTATGCACCATCCGAATGCCGTTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-174)

Prokaryotic N-terminal methylation motif (N_methyl)

(5-29)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

56.614

100

0.615
  pilA2 Legionella pneumophila str. Paris

42.012

97.126

0.408
  pilA2 Legionella pneumophila strain ERS1305867

41.42

97.126

0.402
  comP Acinetobacter baylyi ADP1

37.989

100

0.391
  pilA Ralstonia pseudosolanacearum GMI1000

36.872

100

0.379
  pilE Neisseria gonorrhoeae MS11

37.143

100

0.374
  pilE Neisseria gonorrhoeae strain FA1090

37.427

98.276

0.368

Multiple sequence alignment