Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ALDI51_RS05225 Genome accession   NZ_AP024172
Coordinates   1112836..1114575 (-) Length   579 a.a.
NCBI ID   WP_094434747.1    Uniprot ID   A0A3R7HQH8
Organism   Alicycliphilus denitrificans strain I51     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1107836..1119575
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ALDI51_RS05195 (ALDI51_10340) - 1108555..1108980 (+) 426 WP_201397465.1 NUDIX domain-containing protein -
  ALDI51_RS05200 (ALDI51_10350) - 1109043..1109249 (-) 207 WP_201397466.1 DNA gyrase inhibitor YacG -
  ALDI51_RS05205 (ALDI51_10360) zapD 1109251..1110006 (-) 756 WP_094436935.1 cell division protein ZapD -
  ALDI51_RS05210 (ALDI51_10370) coaE 1110084..1110719 (-) 636 WP_201397467.1 dephospho-CoA kinase -
  ALDI51_RS05215 (ALDI51_10380) pilD 1110722..1111597 (-) 876 WP_201397468.1 prepilin peptidase Machinery gene
  ALDI51_RS05220 (ALDI51_10390) pilC 1111597..1112814 (-) 1218 WP_201397469.1 type II secretion system F family protein Machinery gene
  ALDI51_RS05225 (ALDI51_10400) pilB 1112836..1114575 (-) 1740 WP_094434747.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ALDI51_RS05235 (ALDI51_10410) - 1114931..1115914 (-) 984 WP_011804284.1 polyprenyl synthetase family protein -
  ALDI51_RS05240 (ALDI51_10420) rplU 1116087..1116398 (+) 312 WP_011804285.1 50S ribosomal protein L21 -
  ALDI51_RS05245 (ALDI51_10430) rpmA 1116414..1116671 (+) 258 WP_011804286.1 50S ribosomal protein L27 -
  ALDI51_RS05250 (ALDI51_10440) cgtA 1116796..1117869 (+) 1074 WP_201397470.1 Obg family GTPase CgtA -
  ALDI51_RS05255 (ALDI51_10450) proB 1117934..1119073 (+) 1140 WP_201397471.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 579 a.a.        Molecular weight: 63541.77 Da        Isoelectric Point: 5.4688

>NTDB_id=83824 ALDI51_RS05225 WP_094434747.1 1112836..1114575(-) (pilB) [Alicycliphilus denitrificans strain I51]
MASADIAQKVAQPVALPGLARALMSVGKLDQKTAEEIYKKSQTTRTSFIAELTESNAITSSELAHTVSSIFGAPLVDVEA
LDRQRLPKDLLDFKLCQAYRVVPLGKRSNRLIVATADPTNQEAAEKIKFTTQMGVDWIVAEYHKLIQLVDATTKSGSETL
DSIVNSGDFEFGDISVEEASEENSEAAPNEVEDAPVVRFLHKMLLDAFNMRASDLHFEPYEHNYRVRFRIDGELREIASP
PIAIKEKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKMGIDALGYEPEEK
ERLLQAISRPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFATALKAFLRQD
PDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPQCKAPADI
PHETLLEAGYKEEEIDGSWVTYRPVGCSACNNGYKGRVGIYQVMPVSEEIQRIILRDGSALDIAEQARLEGVRSLRESGL
HKAKAGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1740 bp        

>NTDB_id=83824 ALDI51_RS05225 WP_094434747.1 1112836..1114575(-) (pilB) [Alicycliphilus denitrificans strain I51]
ATGGCTTCCGCAGACATCGCACAGAAAGTGGCTCAACCCGTCGCGCTACCCGGCCTGGCGCGCGCGCTGATGTCCGTGGG
CAAGCTGGACCAGAAAACCGCCGAGGAAATCTACAAGAAGTCACAAACGACGCGCACCAGCTTCATTGCCGAACTGACCG
AGTCCAACGCGATCACCTCCAGCGAGCTCGCCCACACGGTGTCCTCCATCTTCGGGGCGCCGCTGGTGGATGTGGAGGCG
CTCGACCGCCAGCGCCTGCCGAAGGACCTGCTGGATTTCAAGCTGTGCCAGGCCTACCGGGTGGTTCCCCTGGGCAAGCG
CAGCAACCGCCTGATCGTGGCGACGGCCGACCCGACCAACCAGGAGGCTGCGGAAAAGATCAAGTTCACCACGCAGATGG
GCGTGGACTGGATCGTGGCCGAATACCACAAGCTGATCCAGCTGGTGGACGCGACCACGAAGAGCGGCAGCGAGACCCTT
GACAGCATCGTCAACAGCGGCGACTTCGAGTTTGGCGACATCTCCGTCGAGGAAGCCTCGGAGGAGAACTCGGAAGCCGC
ACCGAACGAGGTCGAGGACGCGCCCGTCGTCAGGTTCCTGCACAAGATGCTGCTGGATGCGTTCAACATGCGCGCATCCG
ACCTGCATTTCGAGCCGTACGAGCACAACTACCGCGTGCGCTTTCGCATCGACGGCGAGCTCAGGGAAATCGCGTCCCCT
CCGATCGCCATCAAGGAGAAGCTGGCATCGCGCATCAAGGTGATCTCCCGCCTGGACATCTCGGAGAAGCGCGTGCCGCA
GGACGGCCGCATGAAGCTCAAGGTGGGGCCGGACCGCGTGATCGATTTCCGCGTGAGCACCCTGCCCACGCTCTTCGGCG
AGAAGATCGTGATCCGTATCCTCGATCCGAGCAGCGCCAAGATGGGCATCGACGCGCTCGGCTACGAACCCGAGGAGAAG
GAGCGCCTGCTCCAGGCCATCAGCCGGCCCTACGGCATGATCCTGGTGACGGGCCCCACGGGGTCGGGCAAGACGGTCTC
GCTCTACACCTGCCTGAACCTGCTGAACAAGCCCGGAGTCAACATCGCCACGGCGGAAGACCCCTCGGAAATCAACCTGC
CGGGCGTGAACCAGGTGAACGTGAACGAAAAGGCGGGGCTGACCTTCGCCACGGCCCTGAAGGCGTTCCTGCGCCAGGAT
CCGGACATCATCATGGTGGGCGAAATCCGCGACCTGGAGACCGCCGACATCTCCATCAAGGCCGCCCAGACGGGCCACCT
GGTGCTGTCCACGCTGCACACCAACGACGCGCCGACCACGCTCACGCGCATGCGCAACATGGGCATCGCGCCCTTCAACA
TCGCATCCAGCGTGATCCTGATCACCGCGCAGCGGCTGGCGCGGCGCCTGTGCCCCCAGTGCAAGGCGCCGGCCGACATA
CCGCACGAGACCCTGCTGGAGGCGGGCTACAAGGAAGAAGAGATCGACGGCTCCTGGGTCACCTACCGGCCCGTCGGCTG
CTCGGCCTGCAACAACGGGTACAAGGGCCGCGTGGGCATCTACCAGGTGATGCCGGTGTCCGAGGAAATCCAGCGCATCA
TCCTGCGCGACGGCAGTGCCCTGGACATCGCCGAGCAGGCCCGCCTGGAGGGCGTGCGCAGCCTGCGCGAGTCGGGGCTG
CACAAGGCGAAGGCGGGATTGACCTCCCTGGAAGAGGTCCTGGCCGTCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(202-469)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3R7HQH8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

53.723

97.409

0.523
  pilB Acinetobacter baylyi ADP1

52.265

99.136

0.518
  pilF Neisseria gonorrhoeae MS11

51.943

97.755

0.508
  pilB Legionella pneumophila strain ERS1305867

49.381

97.582

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

46.032

97.927

0.451
  pilB Vibrio cholerae strain A1552

45.234

99.655

0.451
  pilB Vibrio campbellii strain DS40M4

44.779

97.582

0.437
  pilF Thermus thermophilus HB27

40.63

98.618

0.401
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.075

92.228

0.37

Multiple sequence alignment