Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QN096_RS05660 Genome accession   NZ_CP126342
Coordinates   1198839..1200542 (-) Length   567 a.a.
NCBI ID   WP_282886667.1    Uniprot ID   -
Organism   Pseudomonas otitidis strain TL17     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1193839..1205542
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QN096_RS05620 (QN096_05620) - 1194005..1194430 (+) 426 WP_284547960.1 GNAT family N-acetyltransferase -
  QN096_RS05625 (QN096_05625) - 1194427..1194915 (+) 489 WP_284547961.1 FAD/FMN-containing dehydrogenase -
  QN096_RS05630 (QN096_05630) - 1194912..1195598 (+) 687 WP_044406853.1 energy-coupling factor ABC transporter permease -
  QN096_RS05635 (QN096_05635) - 1195710..1195925 (+) 216 WP_160481384.1 hypothetical protein -
  QN096_RS05640 (QN096_05640) yacG 1195938..1196135 (-) 198 WP_142011979.1 DNA gyrase inhibitor YacG -
  QN096_RS05645 (QN096_05645) coaE 1196132..1196743 (-) 612 WP_284547962.1 dephospho-CoA kinase -
  QN096_RS05650 (QN096_05650) pilD 1196740..1197612 (-) 873 WP_074973060.1 A24 family peptidase Machinery gene
  QN096_RS05655 (QN096_05655) pilC 1197615..1198832 (-) 1218 WP_284547963.1 type II secretion system F family protein Machinery gene
  QN096_RS05660 (QN096_05660) pilB 1198839..1200542 (-) 1704 WP_282886667.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QN096_RS05665 (QN096_05665) - 1200721..1201197 (-) 477 WP_074973068.1 pilin -
  QN096_RS05670 (QN096_05670) tfpZ 1201473..1202222 (+) 750 WP_284547964.1 TfpX/TfpZ family type IV pilin accessory protein -
  QN096_RS05680 (QN096_05680) nadC 1202434..1203282 (-) 849 WP_284547965.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62563.64 Da        Isoelectric Point: 5.3365

>NTDB_id=837751 QN096_RS05660 WP_282886667.1 1198839..1200542(-) (pilB) [Pseudomonas otitidis strain TL17]
MNELSTLTGLAKQLVLNNLLDETTAQQAQLQAQRNKLPLVTYLTQNKLVKGRVLVELISDQFGIAMLDLSCIDKDAIPRD
TVSEKLARQHRVLPLFKRGNKLFIATSDPSNHQAVTDVQFSTGLSTEALLVEDDKLGDAIEKFFDNTDNGLSDLGDVDLD
NVDIVSADEDKQDSGASNDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYERTYRVRFRTDGILHEVARPPIQLAPRISAR
LKVMASLDISERRKPQDGRIKMKISKTKAIDFRVNTLPTLWGEKIVMRILDPTSAQMGIDALGYEDDQKELYMNALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTADVNISTAEDPVEINLEGINQVNVNPKQGMDFSQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCSACKKPVDVPRDVLLKEGFP
EDKIGTFKIYGPVGCENCKGGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARRDGFNDLRMSGLLKAMQGVTSLEE
INRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=837751 QN096_RS05660 WP_282886667.1 1198839..1200542(-) (pilB) [Pseudomonas otitidis strain TL17]
ATGAACGAGCTCTCCACCCTGACCGGTCTGGCAAAGCAACTGGTCCTGAATAATCTCCTGGACGAGACCACGGCTCAGCA
GGCTCAGCTCCAGGCCCAACGCAACAAACTCCCCCTTGTCACCTACCTGACTCAGAACAAGCTGGTGAAAGGCCGGGTGT
TGGTGGAACTGATCAGCGACCAGTTCGGTATTGCCATGCTCGACCTGAGCTGCATCGACAAGGACGCGATACCTCGCGAT
ACGGTGAGCGAAAAACTGGCACGCCAGCACCGCGTGCTGCCCCTGTTCAAGCGCGGCAACAAACTGTTCATTGCAACGTC
CGACCCTTCCAATCACCAGGCCGTGACCGATGTGCAGTTCAGCACTGGCCTCAGCACCGAGGCTCTGCTGGTCGAGGACG
ACAAGCTCGGTGATGCGATCGAGAAGTTCTTCGACAATACCGACAACGGGCTGTCGGATCTGGGCGACGTCGACCTCGAT
AATGTCGATATCGTAAGCGCGGATGAAGACAAACAGGACTCTGGGGCCAGCAACGACGCCGACGATGCACCGGTGGTGCG
TTTCGTCAATAAGATGCTCCTGGACGCCATCAAGGGCGGCTCGTCTGACCTGCACTTTGAACCGTACGAACGCACCTACC
GTGTGCGCTTTCGTACCGATGGCATCCTGCACGAAGTGGCCAGGCCACCGATACAACTGGCCCCGCGCATCTCTGCACGC
CTGAAGGTCATGGCCAGTCTGGATATCTCCGAACGGCGCAAACCGCAGGACGGACGGATCAAGATGAAAATCTCCAAGAC
CAAGGCTATCGATTTTCGCGTCAATACCCTTCCAACTCTCTGGGGCGAGAAGATTGTAATGCGGATTCTCGACCCTACCA
GCGCACAGATGGGCATCGATGCCCTTGGTTACGAAGACGATCAGAAAGAACTGTACATGAATGCCCTGAAACAGCCCCAG
GGCATGATTCTGGTTACGGGCCCCACCGGTTCAGGCAAAACGGTTTCCCTTTATACAGGCCTGAACATCCTGAACACGGC
GGACGTCAACATCTCCACCGCTGAAGACCCGGTTGAAATCAACCTGGAAGGTATCAACCAGGTCAATGTCAATCCCAAAC
AAGGCATGGACTTCTCCCAAGCCCTGCGCGCCTTCCTGCGCCAAGACCCTGACATCATCATGGTGGGTGAGATCCGCGAC
CTGGAAACCGCCGAAATTGCTATTAAGGCCGCGCAAACCGGCCACATGGTCATGTCCACGCTACACACCAACAGCGCGGC
CGAAACGCTGACCCGCCTGCGCAACATGGGCGTGCCGGCCTTCAACATCGCCACTTCGGTCAACCTGATTATTGCCCAGC
GCCTGGCTCGCAAGCTCTGCAGCGCCTGCAAGAAGCCAGTGGACGTCCCACGGGATGTGCTGCTGAAAGAAGGTTTCCCG
GAAGACAAGATTGGTACCTTCAAGATTTATGGCCCGGTGGGCTGCGAGAACTGCAAAGGTGGTTACAAGGGCCGGGTTGG
TATTTATGAAGTGGTTAAAAACACGCCTGCCCTGCAACGCATTATCATGGAGGAAGGTAACTCCATTGAAATCGCCGAGC
AGGCTCGCCGGGATGGTTTTAATGATTTGCGCATGTCAGGCCTGCTGAAGGCCATGCAGGGCGTAACCAGCCTCGAGGAA
ATCAACCGCGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.929

99.471

0.596
  pilB Acinetobacter baylyi ADP1

59.325

99.295

0.589
  pilB Legionella pneumophila strain ERS1305867

56.283

99.647

0.561
  pilF Neisseria gonorrhoeae MS11

48.862

100

0.492
  pilB Vibrio cholerae strain A1552

49.643

98.765

0.49
  pilB Vibrio campbellii strain DS40M4

48.307

98.942

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

51.65

90.829

0.469
  pilB/pilB1 Synechocystis sp. PCC 6803

39.245

93.474

0.367
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.163

92.769

0.363